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Scanning Electron Microscope Studies On the Free-Living Nematode Panagrellus Silusiae (Rhabditidae)

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For more content, see Nematology.

The nematode, Panagrellus silusiae, was prepared for scanning electron microscope observation at various times during postembryonic development by fixation in cold acrolein or glutaraldehyde, followed with post-fixation in cold OsO4, immersion in liquid nitrogen, sublimation and vacuum coating with a thin layer of gold. There are no striking changes in the basic surface topology during postembryonic maturation except for the addition of cuticular elaborations related to sexual functions and the development of minor surface features such as warts, plaques and punctations in the adult organisms. The annule periodicity in certain regions changes during maturation. These changes may denote a structural reinforcement of the cuticle of the mature nematodes. The buccal vestibule has a localized ribbed-wall in the anterior portion and irregular dentition in the posterior regions. The occurrence of ecdysis could not be completely authenticated although some aspects of the process may have been observed.

Affiliations: 1: Department of Biology, University of Waterloo, Waterloo, Ontario, Canada


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