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Growth of Nematodes in Defined Medium Containing Hemin and Supplemented With Commercially Available Proteins

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For more content, see Nematology.

It has been shown that several free-living nematodes, the insect parasite Neoaplectana carpocapsae, and the plant parasite Aphelenchus avenae, will grow and reproduce in axenic culture in defined medium containing hemin and supplemented with γ-globulin. In addition, other commercially available proteins were tested with Caenorhabditis briggsae and A. avenae. Activity was less than that of liver growth factor. Proper precipitation of the protein was important in determining the effectiveness of the supplements for C. briggsae. Hemin added to growth factor had no stimulatory effect. These results extend the range of proteins suitable as supplements for growing nematodes.

Affiliations: 1: Clinical Pharmacology Research Institute, 2030 Haste Street, Berkeley, California 94704, U.S.A.


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