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Culturing, Embryology and Life History Studies On the Lance Nematode, Hoplolaimus Indicus

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For more content, see Nematology.

Two populations of Hoplolaimus indicus were cultured monoxenically on excised roots of Sorghum vulgare var. CSH 1 (jowar) in nutrient agar. Prior feeding was necessary for oviposition and development of successive postembryonic stages. The first molt occurred within the egg and the development outside the egg consisted of three larval stages and an adult stage with the three usual molts. The life cycle at a temperature of 28°-32°C from egg to egg stage was completed at 27-36 days and from egg stage to male at 25-27 days. Sex differentiation was indicated in the second molt by the presence of four specialized ventral chord nuclei present only in female larvae.

Affiliations: 1: Division of Nematology, Indian Agricultural Research Institute, New Delhi 12, India


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