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Techniques for Manipulating Small Nematodes

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image of Nematologica
For more content, see Nematology.

Details of construction and operation are given for some apparatus used to observe and manipulate small soil nematodes under the microscope. An observation cell is simply made from rigid P.V.C. Its open sides allow insertion of microtools and depth can be varied in steps. Glass suction cannulae of 20 to 50 μ aperture, connected to syringes, hold nematodes securely. Finer microcannulae (5 to 10 μ aperture) can be inserted through the cuticle for pressure recording. Microdissection knives are made from plate glass slivers fused or glued to glass handles.

Affiliations: 1: Rothamsted Experimental Station, Harpenden, Herts., England


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