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Factors Influencing the Number of Larvae of Heterodera Avenae Invading Barley Seedlings in Vitro

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The influence of inoculum density, duration of exposure, temperature, distance from root tips, and number of root tips on invasion by larvae of Heterodera avenae was studied to try to reduce variability in the numbers invading barley seedlings. The numbers of larvae of Heterodera avenae invading root tips of barley plants (cv. Clipper) increased with inoculum density, time, and increasing numbers of root tips. The optimum temperature for invasion was 20°, and larvae moved randomly on agar. The behaviour of larvae at root tips was observed, and interference between larvae occurred. Many larvae emerged from roots after invasion. The number of larvae establishing within a root was influenced by the number invading - the number leaving roots increased with the number invading. Larvae had the ability to invade roots twice without loss of infectivity.

Affiliations: 1: Department of Plant Pathology, Waite Agricultural Research Institute, University of Adelaide, South Australia

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/content/journals/10.1163/187529276x00238
1976-01-01
2016-12-06

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