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The Influence of Sample Size On Recovery of Pratylenchus Loosi From Tea Feeder Roots

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The number of Pratylenchus loosi Loof recovered from tea feeder roots by a modified Baermann funnel technique, showed an inverse relationship to sample size, and it was found that polyphenols from tea feeder root macerates appeared to reduce recovery of nematodes. The removal of polyphenols from the sieve solution improved recovery significantly and the use of increasingly higher concentrations of polyphenol-free root extracts, as sieve solution, improved recovery above that of distilled water. These findings suggest the presence in the root of compounds which in the absence of polyphenols, help to improve recovery of nematodes by this technique. The short recovery period (16 h) makes it unlikely that microbial contaminants had some effect.

Affiliations: 1: Tea Research Institute of Sri Lanka, Talawakelle, Sri Lanka


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