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Three species of free-living nematodes; Pelodera sp., Acrobeloides sp., and Panagrellus redivivus, were tested for their ability to reproduce in the presence of various concentrations of diflubenzuron. The nematodes were cultured on an oatmeal medium fortified with 0.01 to 10,000 ppm diflubenzuron. After 10 days the numbers of larvae in test treatments were compared to the control. On medium containing 10 ppm diflubenzuron the populations, expressed as a percent of the control, were 5, 47, and 94 for Pelodera, P. redivivus and Acrobeloides, respectively; on media containing 100 ppm the population of Acrobeloides was only 3% of the control. Since diflubenzuron was not found to be toxic to the larvae and because it is a specific inhibitor of chitin synthetase the results suggest that diflubenzuron inhibited chitin synthesis in the egg shells thus effectively inhibiting reproduction.