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The Influence of Tea Root Saponins On the Recovery of Pratylenchus Loosi From Tea Feeder Roots

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image of Nematologica
For more content, see Nematology.

A highly significant improvement in the recovery of Pratylenchus loosi Loof from tea feeder roots by a modified Baerman funnel technique was observed with increasing concentrations of small amounts of tea root saponins (1-10 ppm) in the sieve water. Higher concentrations of saponins (>10 ppm), retarded recovery. The improved recovery with weak solutions of saponins was found to be reversed when the latter were bound to sterols. It is suggested that since free saponins have a tendency to bind to cell wall sterols, small concentrations could weaken cell wall membranes and this effect is likely to have facilitated a better migration of nematodes from root tissue. Higher concentrations, on the other hand, could affect nematode tissue themselves and are therefore likely to affect their mobility and consequently their recovery.

Affiliations: 1: Tea Research Institute of Sri Lanka, Talawakelle, Sri Lanka


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