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A Quantitative Method for Extracting Resting Spores of Two Nematode Parasitic Fungi, Nema Tophthora g yNophila and Verticillium Chlamydosporium, From Soil

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A method is described for quantitative extraction of Nematophthora gynophila and Verticillium chlamydosporium spores from soil. These two species of fungi can prevent populations of the cereal cyst-nematode, Heterodera avenae, increasing when susceptible cereal hosts are grown. The soil sample is wet sieved and the spores separated by centrifugation on a high specific gravity solution of magnesium sulphate. The spore suspension is counted directly under a compound microscope (125 x ). About 80% of spores added to three soils of different texture were recovered. In a survey of 23 soils, spore numbers of both fungi were large where H. avenae failed to multiply on susceptible hosts, and small where the nematode was increasing or causing damage.

Affiliations: 1: Rothamsted Experimental Station, Harpenden, Herts., England

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/content/journals/10.1163/187529281x00566
1981-01-01
2016-12-08

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