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A Study of Mineral Requirements in Caenorhabditis Elegans

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For more content, see Nematology.

The quantities of Mg+2, Na+ , K+ , Mn+2, Ca+ and Cu+2 required by the free-living nematode C. elegans were determined. An individual mineral deficiency was developed by deleting the mineral from the basal medium. Quantitative requirements of individual minerals were determined respectively by adding each mineral at various concentrations to the depleted medium. Serial subcultures and biological pre-treated media were used for the development of Mn+2, Ca+2 and Cu+2 deficiencies. It was found that most C. elegans were supported at 73 μg/ml Mg+2, 300μg/ml Na+, 530 μg/ml K+ , 6.3 μg/ml Mn+2,1500 μg/ml Ca + 2 and 7.2 μg/ml Cu+2.

Affiliations: 1: Department of Nutrition and Food Science, San Jose State University, San Jose, California 95192, U.S.A.; 2: Department of Nutritional Sciences, University of California, Berkeley, California 94720, U.S.A.


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