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A Technique for the Cryopreservation of Viable Juveniles of Meloidogyne Graminicola

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For more content, see Nematology.

Juveniles of Meloidogyne graminicola were successfully preserved in liquid nitrogen using a simple two-step cooling technique with ethanediol (ethylene glycol) as a cryoprotectant. Nematodes were first incubated in 10% (v/v) ethanediol at 37°C for 15 min and then in cold 40% (v/v) ethanediol for 30 or 45 min before freezing in liquid nitrogen to -196°C. The treatments reduced numbers of active nematodes but those juveniles that recovered after thawing infected rice roots and produced large populations of females and eggs after 40 days.

Affiliations: 1: Commonwealth Institute of Parasitology, 395A Hatfield Road, St. Albans, Hertfordshire, U.K.; 2: London School of Hygiene and Tropical Medicine, Winches Farm, 395 Hatfield Road, St. Albans, Hertfordshire, U.K


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