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Observations On the Curation of Plant and Soil Nematodes

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For more content, see Nematology.

Observations are made on the curation of plant and soil nematodes by reference to the extensive collection of microscope slides and specimens in the Nematology Department, Rothamsted Experimental Station. Better packaging of specimens in transit is required. The addition of 2 % glycerol to fixatives/preservatives to prevent drying out and aid in the processing of specimens to glycerol is recommended. The cuticles of some nematodes degenerate in TAF fixative which should not be used for long term storage. The metal Cobb-type double cover glass slides are more convenient for transport and storage; a longer base cover glass improves clearance for microscope objectives. Cover glass supports need to be much more extensive than in the past to avoid the eventual flattening of specimens. The wax ring seal/support system is quite effective but a secondary seal and cover glass support are recommended. Immersion oil damages some seals and should be removed immediately after use. The drying out of slide mounts is a problem; specimens keep better in glycerol in vials than on slides. Slide mounts should be adequately and clearly labelled in water/oil-proof ink; type slides should be clearly indicated.

Affiliations: 1: Nematology Department, Rothamsted Experimental Station, Harpenden, Herts., AL5 2JQ, England


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