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Xenic Culture of Stelnernema Kushidai (Nematoda: Steinernematidae) On Artificial Media

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For more content, see Nematology.

S. kushidai was xenically propagated on dog food/agar and pig offal/agar media supplemented with peptone (1.2%). It propagated to a lesser extent on Wouts' lipid agar medium. The nematode was also xenically propagated on a medium of soluble starch, D-glucose, lard, peptone, yeast extract, agar and distilled water. pH and media composition required for optimal production of the nematode were examined. Maximum yield of infective juveniles was on soluble starch (0.6%), D-glucose (1.0%), lard (3.0%), peptone (1.5%), yeast extract (1.5%), agar (0.3 to 1.0%) and 33 mM Bis-Tris buffer (pH 6.7).

Affiliations: 1: Forestry and Forest Products Research Institute, P.O. Box 16, Tsukuba Norin Kenkyu Danchi, Ibaraki 305, Japan; 2: Oji Institute for Forest Tree Improvement, Kameyama Breeding Station, Oji Paper Co. Ltd., Nobono-cho, Kameyama, Mie 519-02, Japan


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