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Effects of Inoculum Size, Temperature and Time On in Vitro Production of Steinernema Carpocapsae Agriotos

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For more content, see Nematology.

Effects or interactions of bacterial and nematode inoculum sizes, temperature and culture time on the growth and yield of Steinernema carpocapsae Agriotos were established in a sponge culture system with an artificial medium containing yeast extract, soy flour, egg and lard in 500 ml flasks. Nematode inocula were added to 50 g of the autoclaved medium, 3 days after incubating the medium with primary form (phase) of the symbiotic Xenorhabdus nematophilus bacteria at 15, 19, 23, 27, 31 °C. Yields of infective juveniles were significantly affected by nematode inoculum size ranging from 3x 102 to 3x 106 per flask and by culture time ranging from 8 to 24 days, but not by bacterial inoculum size. The normal temperature range for S. carpocapsae was 19-27°C, with 19-23°C supporting optimal growth. Negative interactions were observed between temperature and culture time, between temperature and nematode inoculum size, and between nematode inoculum size and culture time. The highest yield of 38x 106 per flask was obtained with 3× 105 inoculum in 20 days at 19°C. The results suggest that for economic production of nematodes within the optimal temperature range, nematode inoculum and culture time must be kept in balance for maximum utilization of the medium and for maximum formation of infective juveniles.

Affiliations: 1: Guangdong Entomological Institute, Guangzhou 510260, China


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