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Phylogeography of Hepialus humuli (L.) (Lepidoptera: Hepialidae) in Europe: short distance vs. large scale postglacial expansions from multiple Alpine refugia and taxonomic implications

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We explore the phylogeography of the common ghost moth, Hepialus humuli (Linnaeus) in Europe based on 1451 bp Cytochromeoxydase Subunit 1 (COI) mtDNA and 617 bp Ribosomal protein Subunit 5 (RpS5) ntDNA with special focus on populations in the Alps and surrounding regions, as well as northern Europe. While RpS5 fails to recover any phylogeographic signal, COI reveals a remarkable pattern with central European populations separated in four well-defined groups. The most divergent group is restricted to northern Italy and southern Austria and geographically isolated from the others; one group is found only in the central-northern region south of Lake Constance (Liechtenstein, western Austria) and co-occurs with the two other groups, from north-eastern Alps and north-western Alps respectively. We conclude that the southern and central groups are relicts from a previous Pleistocene glacial maximum, whereas the two latter groups were isolated during the last glacial maximum in a western and an eastern refugium respectively, the exact extends of these refugia are uncertain. The central group has subsequently interbred with the two other northern groups and probably only exists today as ancient mtDNA haplotypes. The north-western and north-eastern groups have spread considerably and overlap over a large part of their range in the Alps and surrounding areas. Following the last glacial maximum, the north-western group spread into western Europe as far as Normandy, but the English Channel has apparently acted as a dispersal barrier. The north-eastern group spread into eastern and northern Europe, including Scandinavia, and possibly into the Balkans as well. The British Isles as well as the North Atlantic islands groups, the Faroese and Shetlands were colonised from southern Scandinavia or northern Germany, likely via Doggerland. Despite the deep divergence in mtDNA between the populations in Italy and southern Austria, and the remaining populations, there are no consistent morphological differences, and we conclude that there is no evidence that the southern populations should be considered a separate species. Although the populations in the Shetland and Faroese islands are phenotypically distinct from most other populations, we find no genetic or genitalia morphological differences between these populations and the rest. We therefore conclude that they display what can be termed cryptic genetic homogeneity. As the phenotypic variation is not unique to these populations either, we synonymise the North Atlantic subspecies H. humuli thulensis Newman syn.n. with H. humuli humuli.

Affiliations: 1: aDepartment of Life Sciences, Natural History Museum, Cromwell Road, London SW7 5BD, UK; 2: bTiroler Landesmuseen Betriebsges.m.b.H., Naturwissenschaftliche Sammlungen, Feldstr. 11A, A-6020 Innsbruck, Austria

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    • Publication Date : 31 July 2014
    • DOI : 10.1163/1876312x-44032104_001
    • File Size: 2181696
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1. Andersson S. ,, Rydell J. ,& Svensson M.G.E. ( 1998) "Light, predation and the lekking behaviour of the ghost swift Hepialus humuli(L.) (Lepidoptera: Hepialidae)". Proceedings of the Royal Society of London Series B: Biololgical Sciences Vol 265: 13451351.
2. Askew R. R. ( 2004) The Dragonflies of Europe (revised edition). Harley Books, Colchester, 308pp.
3. Bandelt H.J. ,, Forster P. ,& Rohl A. ( 1999) "Median-joining networks for inferring intraspecific phylogenies". Molecular Biology and Evolution Vol 16: 3748.
4. Bertaccini E. ,, Fiumi G. ,& Provera P. ( 1997) Bombici e Sfingi d’Italia (Lepidoptera Heterocera) . Volume II. Natura, Giuliano Russo Editore, Monterenzio, 256pp.
5. Brochmann C. ,, Gabrielsen T.M. ,, Nordal I. ,, Landvik J.Y. ,& Elven R. ( 2003) "Glacial survival or tabula rasa? The history of North Atlantic biota revisited". Taxon Vol 52: 417450.
6. Brower A.V.Z. ( 1994) "Rapid morphological radiation and convergence among races of the butterfly Heliconius erato– inferred from patterns of mitochondrial-DNA evolution". Proceedings of the National Academy of Sciences of the United States of America Vol 91: 64916499.
7. Cognato A.I. ( 2006) "Standard percent DNA sequence difference for insects does not predict species boundaries". Journal of Economic Entomology Vol 99: 10371045.
8. Dahl L. ( 1954) "Lepidoptera from the Faroes". List II. Frodskaparrit Vol 3: 128154.
9. de Juana E. ,( 1994) "Family Tetraonidae (Grouse)". In: del Hoyo J. ,, Elliott A. ,& Sargata J. , (Eds.) Handbook of the Birds of the World . Vol. Vol 2. New World Vulturesto Guineafowl. Lynx Edicions, Barcelona, pp. 376410.
10. de Lattin G. ( 1964) "Die Verbreitung der sibirischen Faunenelemente der Lepidopteren in der Westpaläarktis". Natur und Museum Frankfurt am Main Vol 94: 505514.
11. de Lattin G. ( 1967) Grundriss der Zoogeographie . Verlag Gustav Fischer, Stuttgart, 602pp.
12. Delforge P. ( 2006) Orchids of Europe, North Africa and the Middle East . A & C Black, London, 640pp.
13. Dupuis J. R. ,, Roe A. D. ,& Sperling F. A. H. ( 2012) "Multi-locus species delimitation in closely related animals and fungi: one marker is not enough". Molecular Ecology Vol 21: 44224436.
14. Folmer O. ,, Black M. ,, Hoeh W. ,, Lutz R. ,& Vrijenhoek R. ( 1994) "DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates". Molecular Marine Biology and Biotechnology Vol 3: 294299.
15. Freina J.J. ,de & Witt T.J. ( 1990) Die Bombyces und Sphinges der Westpaläarktis (Insecta, Lepidoptera) . Vol. II. Forschung & Wissenschaft, München, 140pp., 14 pls, 57 maps.
16. Gaffney V. L. ,, Thomson K. ,& Fitch S. ( 2007) Mapping Doggerland: the Mesolithic landscapes of the southern North Sea . Archaeopress, Oxford, England. 131pp.
17. Garcia A.G. ,, Llorca J.J. ,, Planas A.M. ,, De-Gregoria J.J.P. ,& Ferrer-Vidal I.R. ( 1982) "Revisió de la família Hepialidae Stephens, 1829 Lepidoptera: Homoneura) a Catalunya". Butlletí de la Institució Catalana d’Història Natural Vol 49: 111117.
18. Goloboff P.A. ,, Farris J.S. ,& Nixon K.C. ( 2008) "TNT, a free program for phylogenetic analysis". Cladistics Vol 24: 774786.
19. Hammouti N. ,, Schmitt T. ,, Seitz A. ,, Kosuch J. ,& Veith M. ( 2010) "Combining mitochondrial and nuclear evidences: a refined evolutionary history of Erebia medusa(Lepidoptera: Nymphalidae: Satyrinae) in Central Europe based on the COI gene". Journal of Zoological Systematics and Evolutionary Research Vol 48: 115125.
20. Hansen J. ,& Jensen J.-K. ( 2005) "Colour morphs of the ghost moth Hepialus humuliL. (Lepidoptera, Hepialidae) in the Faroe Islands". Entomologiske Meddelelser Vol 73: 123130.
21. Heath J. ,( 1976) "Hepialidae". In: Heath J. (Ed.) The moths and butterflies of Great Britain and Ireland . Volume Vol 1. Micropterigidae – Heliozelidae. Harley Books, Colchester, pp 167170.
22. Hebert P.D.N. ,, Cywinska A. ,, Ball S.L. ,& DeWaard J.R. ( 2003) "Biological identifications through DNA barcodes". Proceedings of the Royal Society of London Series B: Biololgical Sciences Vol 270: 313321.
23. Heikkila M. ,, Kaila L. ,, Mutanen M. ,, Pena C. ,& Wahlberg N. ( 2012) "Cretaceous origin and repeated tertiary diversification of the redefined butterflies". Proceedings of the Royal Society of London Series B: Biololgical Sciences Vol 279: 10931099.
24. Hewitt G.M. ( 1999) "Post-glacial re-colonization of European biota". Biological Journal of the Linnean Society Vol 68: 87112.
25. Hewitt G.M. ( 2004) "Genetic consequences of climatic oscillations in the Quaternary". Philosophical Transactions of the Royal Society of London Series B: Biological Sciences Vol 359: 183195.
26. Hoffmeyer S. ( 1948) De danske Spindere , 2nd edn. Universitetsforlaget, Århus, 194pp.
27. Huemer P. ( 2011) "Pseudo-endemism and cryptic diversity in Lepidoptera – case studies from the Alps and the Abruzzi". Eco Mont-Journal on Protected Mountain Areas Research and Management Vol 3: 1118.
28. Huemer P. ,& Hebert P.D.N. ( 2011) "Cryptic diversity and phylogeography of high alpine Sattleria – a case study combining DNA barcodes and morphology (Lepidoptera: Gelechiidae)". Zootaxa Vol 2981: 122.
29. Huemer P. ,& Karsholt O. ( 2011) " Eulamprotes libertinellaauctt. – ein Komplex kryptischer alpiner ˝Kleinschmetterlinge˝ (Lepidoptera, Gelechiidae)?" Entomologische Nachrichten und Berichte Vol 55: 217229.
30. Huemer P. ,& Mutanen M. ( 2012) "Taxonomy of spatially disjunct alpine Teleiopsis (Lepidoptera: Gelechiidae) revealed by molecular data and morphology–how many species are there?" Zootaxa Vol 3580: 123.
31. Jakšić P. ,& Ristić G. ( 1999) "New and rare Lepidoptera in Yugoslavia". Acta Entomologica Serbica Vol 4: 6374.
32. Kaaber S. ,, Kristensen N. ,& Simonsen T.J. ( 2009) "Sexual dimorphism and geographical male polymorphism in the ghost moth Hepialus humuli(Lepidoptera: Hepialidae): Scale ultrastructure and evolutionary aspects". European Journal of Entomology Vol 106: 303313.
33. Katoh K. ,, Misawa K. ,, Kuma K. ,& Miyata T. ( 2002) "MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform". Nucleic Acids Research Vol 30: 30593066.
34. Kettlewell B. ( 1973) The Evolution of Melanism. The Study of a Recurring Necessity. With Special Reference to Industrial Melanism in the Lepidoptera . Clarendon Press, Oxford, 423pp.
35. Kodandaramaiah U. ,, Weingartner E. ,, Janz N. ,, Dalen L. ,& Nylin S. ( 2011) "Population structure in relation to host–plant ecology and Wolbachia infestation in the comma butterfly". Journal of Evolutionary Biology Vol 24: 21732185.
36. Lambeck K. ( 1995) "Late Devensian and Holocene shorelines of the British Isles and North-Sea from models of glacio-hydro-isostatic rebound". Journal of the Geological Society Vol 152: 437448.
37. Lempke B.J. ( 1938) "Catalogus der Nederlandsche Macrolepidoptera. III". Tijdschrift voor Entomologie Amsterdam Vol 82: 242304.
38. Leraut P. ( 2006). Moths of Europe . Vol. I. Saturnids, Lasiocampids, Hawkmoths, Tiger Moths. N.A.P. Editions, Verrières le Buisson, 387pp.
39. Maddison W.P. ,& Maddison D.R. ( 2011) "Mesquite: a modular system for evolutionary analysis". Version 2.75, available online at .
40. Mallet J. ( 1984). "Sex roles in the ghost moth Hepialus humuli(L.) and a review of mating in the Hepialidae (Lepidoptera)", Zoological Journal of the Linnean Society Vol 79: 6782.
41. Meier R. ( 2008) "DNA sequences in taxonomy – Opportunities and challenges". New Taxonomy Vol 76: 95127.
42. Mutanen M. ,, Hausmann A. ,, Hebert P.D.N. ,, Landry J.-F. ,, de Waard J.R. ,& Huemer P. ( 2012) "Allopatry as a Gordian Knot for Taxonomists: Patterns of DNA Barcode Divergence in Arctic-Alpine Lepidoptera". Plos One Vol 7( 10): e47214, doi:10.1371/journal.pone.0047214.
43. Nielsen E.S. ( 1988) "The peculiar Asian ghost moth genus BipectilusChu and Wang – Taxonomy and systematic position (Lepidoptera: Hepialidae s.str.)". Systematic Entomology Vol 13: 171195.
44. Nielsen E.S. ,& Kristensen N.P. ( 1989) "Primitive Ghost Moths". Monographs on Australian Lepidoptera Vol 1: 206pp.
45. Nielsen E.S. ,& Scoble M.J. ( 1986) " Afrotheora, a new genus of primitive Hepialidae from Africa (Lepidoptera: Hepialoidea)". Entomologica Scandinavica Vol 17: 2954.
46. Nielsen E.S. ,, Robinson G.S. ,& Wagner D.L. ( 2000) "Ghostmoths of the World: a Global Inventory and Bibliography of the Exoporia (Mnesarchaeoidea and Hepialoidea) (Lepidoptera)". Journal of Natural History Vol 34: 823878.
47. Rabitsch W. ,& Essl F. ( 2009) Endemiten – Kostbarkeiten in Österreichs Pflanzen- und Tierwelt . Naturwissenschaftlicher Verein für Kärnten und Umweltbundesamt GmbH, Klagenfurt und Wien, 924 pp.
48. Ratnasingham S. ,& Hebert P. D.N. ( 2007) "BOLD: The Barcode of Life Data System (". Molecular Ecology Notes Vol 7: 355364.
49. Rebel H. ( 1904) "Studien über die Lepidopterenfauna der Balkanländer.II. Teil. Bosnien und Herzegowina". Annalen des k.k. Naturhistorischen Museums in Wien Vol 19: 97377+ pls. IVV.
50. Ronquist F. ,, Huelsenbeck J. ,& Teslenko M. ( 2011) "Draft Mr Bayes version 3.2 Manual: Tutorials and Model Summaries". Online publication available from the authors.
51. Schaffer M. ( 2008) Channel Islands Lepidoptera: history and compilation of the recorded butterflies and moths 1830–2003 . Published by the author, London. 710pp.
52. Schmidt B.C. ,& Sperling F.A.H. ( 2008) "Widespread decoupling of mtDNA variation and species integrity in Grammia tiger moths (Lepidoptera: Noctuidae)". Systematic Entomology Vol 33: 613634.
53. Schmitt T. ( 2007) "Molecular biogeography of Europe: Pleistocene cycles and postglacial trends". Frontiers in Zoology Vol 4: 11.
54. Schmitt T. ( 2009) "Biogeographical and evolutionary importance of the European high mountain systems". Frontiers in Zoology Vol 6: 9.
55. Schmitt T. ,, Rakosy L. ,, Abadjiev S. ,& Mueller P. ( 2007) "Multiple differentiation centres of a non-Mediterranean butterfly species in south-eastern Europe". Journal of Biogeography Vol 34: 939950.
56. Schmitt T. ,, Muster C. ,& Schönswetter P. ,( 2010) "Are Disjunct Alpine and Arctic-Alpine Animal and Plant Species in the Western Palaearctic Really “Relics of a Cold Past”?"In: Habel J.C. ,& Assmann T. (Eds.) Relict Species: Phylogeography and Conservation Biology . Springer, Berlin, pp. 239252.
57. Schmitt T. ,& Seitz A. ( 2001) "Intraspecific allozymatic differentiation reveals the glacial refugia and the postglacial expansions of European Erebia medusa(Lepidoptera: Nympahlidae)". Biological Journal of the Linnean Society Vol 74: 429458.
58. Schneeweiss G.M. ,& Schönswetter P. ( 2010) "The wide but disjunct range of the European mountain plant Androsace lacteaL. (Primulaceae) reflects Late Pleistocene range fragmentation and post-glacial distributional stasis". Journal of Biogeography Vol 37: 20162025.
59. Schönswetter P. ,, Stehlik I. ,, Holderegger R. ,& Tribsch A. ( 2005) "Molecular evidence for glacial refugia of mountain plants in the European Alps". Molecular Ecology Vol 14: 35473555.
60. Schoville S.D. ,, Stuckey M. ,& Roderick G.K. ( 2011) "Pleistocene origin and population history of a neoendemic alpine butterfly". Molecular Ecology Vol 20: 12331247.
61. Simon C. ,, Frati F. ,, Beckenbach A. ,, Crespi B. ,, Liu H. ,& Flook P. ( 1994) "Evolution, weighting, and phylogenetic utility of mitochondrial genesequences and a compilation of conserved polymerase chain-reaction primers". Annals of the Entomological Society of America Vol 87: 651701.
62. Simonsen T.J. ,, Zakharov E.V. ,, Djernaes M. ,, Cotton A.M. ,, Vane-Wright R.I. ,& Sperling F.A.H. ( 2011) "Phylogenetics and divergence times of Papilioninae (Lepidoptera) with specialreference to the enigmatic genera Teinopalpus and Meandrusa". Cladistics Vol 27: 113137.
63. Stehlik I. ( 2003) "Resistance or emigration? Response of alpine plants to the ice ages". Taxon Vol 52: 499510.
64. Todisco V. ,, Gratton P. ,, Zakharov E.V. ,, Wheat C.W. ,, Sbordoni V. ,& Sperling F.A.H. ( 2012) "Mitochondrial phylogeography of the Holarctic Parnassius phoebus complex supports a recent refugial model for alpine butterflies". Journal of Biogeography Vol 39: 10581072.
65. Tribsch A. ,& Schönswetter P. ( 2003) "Patterns of endemism and comparative phylogeography confirm palaeoenvironmental evidence for Pleistocene refugia in the Eastern Alps". Taxon Vol 52: 477497.
66. Ursenbacher S. ,, Carlsson M. ,, Helfer V. ,, Tegelstrom H. ,& Fumagalli L. ( 2006) "Phylogeography and Pleistocene refugia of the adder ( Vipera berus) as inferred from mitochondrial DNA sequence data". Molecular Ecology Vol 15: 34253439.
67. Varga Z. S. ( 1997) "Das Prinzip der areal-analytischen Methode in der Zoogeographie und die Faunenelemente – Einteilung der europäischen Tagschmetterlinge (Lep. Diurna)". Acta Biologica Debrecina Vol 14: 223285.
68. van Husen D. ( 1997) "LGM and late-glacial fluctuations in the Eastern Alps". Quaternary International Vol 38–39: 109118.
69. Varga Z.S. ,& Schmitt T. ( 2008) "Types of oreal and oreotundral disjunctions in the western Palearctic". Biological Journal of the Linnean Society Vol 93: 415430.
70. Wahlberg N. ,, Leneveu J. ,, Kodandaramaiah U. ,, Pena C. ,, Nylin S. ,, Freitas A.V.L. ,& Brower A.V.Z. ( 2009) "Nymphalid butterflies diversify following near demise at the Cretaceous/Tertiary boundary". Proceedings of the Royal Society of London Series B:Biological Sciences Vol 276: 42954302.
71. Wahlberg N. ,& Wheat C.W. ( 2008) "Genomic outposts serve the phylogenomic pioneers: Designing novel nuclear markers for genomic DNA extractions of lepidoptera". Systematic Biology Vol 57: 231242.
72. Will K.W. ,& Rubinoff D. ( 2004) "Myth of the molecule: DNA barcodes for species cannot replace morphology for identification and classification". Cladistics Vol 20: 4755.
73. Zhou W.G. ,, Rousset F. ,& O’Neill S. ( 1998) "Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences". Proceedings of the Royal Society of London Series B: Biological Sciences Vol 265: 509515.

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