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Full Access Seasonal Variability in Trypsin and α-amylase Activities Caused by the Molting Cycle and Feeding Habits of Juvenile Pink Shrimp Farfantepenaeus duorarum (Burkenroad, 1939)

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Seasonal Variability in Trypsin and α-amylase Activities Caused by the Molting Cycle and Feeding Habits of Juvenile Pink Shrimp Farfantepenaeus duorarum (Burkenroad, 1939)

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In view of the relationship between shifts in diet composition and the activity of digestive enzymes in penaeid shrimp, the present study focused on the analysis of digestive trypsin and α-amylase activities of wild Farfantepenaeus duorarum (Burkenroad, 1939) juveniles and their changes in phenotypic expression, during the molt cycle as endogenous factor and their changes due to different feeding regimes (exogenous factor) in relation with δ 13 C and δ 15 N isotopic signature as an index of food assimilation induced by the seasonal availability of food items in the nursery area. Wild juveniles of F. duorarum were captured from April 2007 to February 2008, in the Celestun coastal lagoon, Yucatan, Mexico. Samplings were carried out considering all quarters of the lunar cycle and in each of the recognized seasons for this region: dry, rainy, and the Nortes (North Wind). Copepods and amphipods were the main source of food for juveniles of F. duorarum. Values of δ 13 C in the muscular tissue were near -20‰hence the feeding regime of F. duorarum in the lagoon was composed by material of marine origin. Isotopic signature differences were found between the three annual seasons. It is an opportunist generalist organism that is located in the 4th trophic level. The digestive enzymatic activities of both trypsin and α-amylase in fresh hepatopancreas tissue showed an interaction between season and molt stages ( p < 0 . 05 ). Activity of the trypsin was highest during the Nortes at molt stage C (140 mU mg-1 HP) and activity of α-amylase was higher in the Nortes at stage B 2 (674 mU mg-1 HP). The amylase/trypsin ratio also showed significant interaction between season and molt stages ( p < 0 . 05 ), with higher values in premolt stages during the rainy and Nortes seasons. Isoforms of these digestive enzymes differed in expression according to the molt stage and also to the season with expression generally being greater at stage C.

Affiliations: 1: 1Posgrado en Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Puerto de Abrigo S/N, Sisal, Hunucmá, Yucatán 97355 México; 2: 2Unidad Multidisciplinaria de Docencia e Investigación de Sisal, Facultad de Ciencias, Universidad Nacional Autónoma de México, Puerto de Abrigo S/N, Sisal, Hunucmá, Yucatán 97355 México; 3: 3Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Puerto de Abrigo S/N, Sisal, Hunucmá, Yucatán 97355 México; 4: 4Ifremer, Centre du Pacifique, BP 7004, Taravao, 98719 Tahiti; 5: 5Facultad de Ciencias, Universidad Nacional Autónoma de México, Puerto de Abrigo S/N, Sisal, Hunucmá, Yucatán 97355 México

In view of the relationship between shifts in diet composition and the activity of digestive enzymes in penaeid shrimp, the present study focused on the analysis of digestive trypsin and α-amylase activities of wild Farfantepenaeus duorarum (Burkenroad, 1939) juveniles and their changes in phenotypic expression, during the molt cycle as endogenous factor and their changes due to different feeding regimes (exogenous factor) in relation with δ 13 C and δ 15 N isotopic signature as an index of food assimilation induced by the seasonal availability of food items in the nursery area. Wild juveniles of F. duorarum were captured from April 2007 to February 2008, in the Celestun coastal lagoon, Yucatan, Mexico. Samplings were carried out considering all quarters of the lunar cycle and in each of the recognized seasons for this region: dry, rainy, and the Nortes (North Wind). Copepods and amphipods were the main source of food for juveniles of F. duorarum. Values of δ 13 C in the muscular tissue were near -20‰hence the feeding regime of F. duorarum in the lagoon was composed by material of marine origin. Isotopic signature differences were found between the three annual seasons. It is an opportunist generalist organism that is located in the 4th trophic level. The digestive enzymatic activities of both trypsin and α-amylase in fresh hepatopancreas tissue showed an interaction between season and molt stages ( p < 0 . 05 ). Activity of the trypsin was highest during the Nortes at molt stage C (140 mU mg-1 HP) and activity of α-amylase was higher in the Nortes at stage B 2 (674 mU mg-1 HP). The amylase/trypsin ratio also showed significant interaction between season and molt stages ( p < 0 . 05 ), with higher values in premolt stages during the rainy and Nortes seasons. Isoforms of these digestive enzymes differed in expression according to the molt stage and also to the season with expression generally being greater at stage C.

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1. Alpuche J. , Pereyra A. , Agundis C. . 2005. "Respuestas bioquímicas de camarones marinos a factores ambientales. Veterinaria Organización S. L., España. VI". Revista Electrónica de Veterinaria REDVET, ISSN 5: 1695-7504. http://www.veterinaria.org/revistas/redvet
2. Arena L. , Cuzon G. , Pascual C. , Gaxiola G. , Soyez C. , van Wormhoudt A. , Rosas C. . 2003. "Physiological and genetic variations in domesticated and wild populations of Litopenaeus vannameifed with different carbohydrates levels". Journal of Shellfish Research Vol 22: 269- 279.
3. Barclay M. C. , Dall W. , Smith D. M. . 1983. "Changes in lipid and protein during starvation and the moulting cycle in the tiger prawn, Penaeus esculentusHaswell". Journal Experimental Marine Biology and Ecology Vol 68: 229- 244. http://dx.doi.org/10.1016/0022-0981(83)90055-2
4. Bernfeld B. 1955. "Sur une méthode de dosage des amylases", pp.  149- 154. In, Colowick S. P. , Kaplan N. O. (eds.), Methods in Enzymology. Academic Press, New York.
5. Boone L. 1931. "A collection of anomuran and macruran Crustacea from the Bay of Panama and the fresh waters of the Canal Zone". Bulletin of the American Museum of Natural History Vol 63( 2): 137- 189.
6. Brêthes J. C. , Parent B. , Pellerin J. . 1994. "Enzymatic activity as an index of trophic resource utilization by the snow crab Chionoecetes opilio(O. Fabricius)". Journal of Crustacean Biology Vol 14: 220- 225. http://dx.doi.org/10.2307/1548902
7. Bouillon S. , Raman A. V. , Dauby P. , Dehairs F. . 2002a. "Carbon and nitrogen stable isotope ratios of subtidal benthic invertebrates in an estuarine mangrove ecosystem (Andhra Pradesh, India)". Estuarine, Coastal and Shelf Science Vol 54: 901- 913. http://dx.doi.org/10.1006/ecss.2001.0864
8. Bouillon S. , Koedam N. , Raman A. V. , Dehairs F. . 2002b. "Primary producers sustaining macro-invertebrate communities in intertidal mangrove forests". Oecologia Vol 130: 441- 448. http://dx.doi.org/10.1007/s004420100814
9. Burkenroad M. D. 1936. "A new species of Penaeusfrom the American Atlantic". Anais de Academia Brasileira de Ciencias, Rio de Janiero Vol 8: 315- 318.
10. Burkenroad M. D. . 1939. "Further observations on Penaeidae on the northern Gulf of Mexico". Bulletin of the Bingham Oceanographic Collection Vol 6( 6): 1- 62.
11. Cara B. , Moyano F. J. , Zambonino J. L. , Fauvel Ch. . 2004. "Actividad tripsina y quimotripsina como indicadores de condición larvaria: estudio de restricción alimentaria y calidad de puesta", pp.  547- 556. In, Conferencias Científica, III Congreso Iberoamericano Virtual de Acuicultura.
12. Carrillo-Farnes O. , Forrellat B. A. , Gonzalez V. R. . 1995. Procedimiento de obtención de hepatopancreatina de camarón. Oficina Cubana de la Propiedad Industrial, 6 pp.
13. Casillas-Hernandez R. , Magallon F. , Portillo G. , Carrillo O. , Nolasco H. , Vega-Villasante F. . 2002. "La actividad de proteasa, amilasa y lipasa durante los estadios de muda del camarón azul Litopenaeus stylirostris ". Revista de Investigaciones Marinas Vol 23: 35- 40.
14. Chong V. C. , Low C. V. , Ichikawa T. . 2001. "Contribution of mangrove detritus to juvenile prawn nutrition: a dual stable isotope study in a Malaysian mangrove forest". Marine Biology Vol 138: 77- 86. http://dx.doi.org/10.1007/s002270000434
15. Christensen V. , Pauly D. . 1992. "ECOPATH II – a software for balancing steady-state ecosystem models and calculating network characteristics". Elsevier Science Publishers B.V., Amsterdam Ecological Modelling Vol 61: 169- 185.
16. Coplen T. B. , Brand W. A. , Gehre M. , Gröning M. , Meijer H. A. J. , Toman B. , Verkouteren R. M. . 2006. "New guidelines for δ 13 C measurements". Annals Chemistry Vol 78: 2439- 2441. http://dx.doi.org/10.1021/ac052027c
17. Cuzon G. , Cahu C. , Aldrin J. F. , Messager J. L. , Stephan G. , Mevel M. . 1980. "Starvation effect on metabolism of Penaeus japonicus ". Procediment World Mariculture Society Vol 11: 410- 423. http://dx.doi.org/10.1111/j.1749-7345.1980.tb00135.x
18. Dall W. , Hill B. J. , Rothlisberg P. C. , Staples D. J. . 1990. "The biology of the Penaeidae", pp.  1- 489. In, Blaxter J. H. S. , Southward A. J. (eds.), Advances in Marine Biology. Vol Vol. 27.
19. de Coursey P. J. 1983. "Biological timing", pp.  107- 162. In, Vernberg F. J. , Vernberg W. B. (eds.), The Biology of Crustacea. Vol Vol. 7. Academic Press, New York.
20. Forrellat B. A. , del Monte M. A. , Estévez L. T. , Boburg C. B. , Nolasco S. H. , Carrillo F. O. . 2004. "Caracterización de lipasas en tres especies de camarones peneidos. Su importancia en la digestión", pp.  767- 776. In, III Congreso Iberoamericano Virtual de Acuicultura.
21. Fry B. 1988. "Food web structure on Georges Bank from stable C, N, and S isotopic compositions". Limnology Oceanography Vol 33: 1182- 1190. http://dx.doi.org/10.4319/lo.1988.33.5.1182
22. Gamboa-Delgado J. , Molina-Poveda C. , Cahu Ch. . 2003. "Digestive enzyme activity and food ingesta in juvenile shrimp Litopenaeus vannamei(Boone, 1931) as a function of body weight". Aquaculture Research Vol 34: 1403- 1411. http://dx.doi.org/10.1111/j.1365-2109.2003.00959.x
23. García-Carreño F. L. , Dimes L. E. , Haard N. F. . 1993. "Substrate-gel electrophoresis for composition and molecular weight of proteinases or proteinaceous inhibitors". Anals Biochemistry Vol 214: 65- 69. http://dx.doi.org/10.1006/abio.1993.1457
24. Garcia-Rodriguez F. J. , De La Cruz-Agüero J. . 2011. "A comparison of indexes for prey importance inferred from otoliths and cephañopod beaks recovered from pinniped scats". Journal of Fisheries and Aquatic Science Vol 6: 186- 193. http://dx.doi.org/10.3923/jfas.2011.186.193
25. Gaxiola G. , Cuzon G. , Ceccaldi H. . 2009. "Digestive physiology in crustacea". In, Broudy G. , Jory D. E. (eds.), The Rising Tide. Proceedigns of Special Session on Sustainable Shrimp Farming2009. World Aquaculture Society, Baton Rouge Lousiana, USA, ISBN 978-I-888807-08-0.
26. Gaxiola G. , Cuzon G. , Garcia T. , Taboada G. , Brito R. , Chimal M. E. , Paredes A. , Soto L. , Rosas C. , van Wormhoudt A. . 2005. "Factorial effects of salinity, dietary carbohydrate and moult cycle on digestive carbohydrases and hexokinases in Litopenaeus vannamei(Boone, 1931)". Comparative Biochemistry and Physiology Vol 140: 29- 39. http://dx.doi.org/10.1016/j.cbpb.2004.10.018
27. Geiger R. , Fritz H. . 1988. "Trypsin", pp.  119- 129. In, Bergmeyer H. U. , Grab M. (eds.), Methods of Enzymatic Analysis. Vol Vol. 5. Chemie Verlag, Weinhein. Academic Press, New York.
28. Hernandez-Guevara N. A. , Ardisson P. L. , Pech D. . 2008. "Temporal trends in benthic macrofauna composition in response to seasonal variation in a tropical coastal lagoon, Celestun, Gulf of Mexico". Marine and Freshwater Research Vol 59: 772- 779. http://dx.doi.org/10.1071/MF07189
29. Herrera-Silveira J. A. 1993. Ecología de los Productores Primarios en la Laguna de Celestún, México. Patrones de Variación espacial y temporal. Ph.D. Diss., Univ. Barcelona.
30. Herrera-Silveira J. A. , Ramirez-Ramirez J. . 1998. "Salinity and nutrients in the coastal lagoons of Yucatan, Mexico". Verh. International. Verein. Limnology Vol 26: 1473- 1478.
31. Herrera-Silveira J. A. , Martin M. , Diaz-Arce V. . 1999. "Phytoplankton variations in four coastal lagoons, Yucatan, Mexico". Revista de Biología Tropical Vol 47: 47- 56.
32. Jones D. A. , Kumlu M. , Le Vay L. , Fletcher D. J. . 1997. "The digestive physiology of herbivorous, omnivorous and carnivorous crustacean larvae: a review". Aquaculture Vol 155: 285- 295. http://dx.doi.org/10.1016/S0044-8486(97)00129-4
33. Klein B. , Moullac L. G. , Sellos D. , Van Wormhout A. . 1996. "Molecular cloning and sequencing of trypsin cDNAs from Penaeus vannamei(Crustacea, Decapoda): use in assessing gene expression during the moult cycle". Journal of Biochemistry and Cell Biology Vol 28: 551- 563. http://dx.doi.org/10.1016/1357-2725(95)00169-7
34. Le Moullac G. , van Wormhoudt A. . 1994. "Adaptation of digestive enzymes to dietary protein, carbohydrate and fibre levels and influence of protein and carbohydrate quality in Penaeus vannameilarvae (Crustacea, Decapoda)". Aquatic Living Ressources Vol 7( 3): 203- 210. http://dx.doi.org/10.1051/alr:1994022
35. Le Moullac G. , Klein B. , Sellos D. , van Wormhout A. . 1996. "Adaptation of trypsin, chymotrypsin and α-amylase to casein level and protein source in Penaeus vannamei(Crustacea Decapoda)". Journal of Experimental Marine Biology and Ecology Vol 208: 107- 125. http://dx.doi.org/10.1016/S0022-0981(96)02671-8
36. Lovett D. L. , Felder D. L. . 1990. "Ontogenetic changes in enzyme distribution and midgut function in developmental stages of Penaeus setiferus(Crustacea, Decapoda, Penaeidae)". Biology Bulletin Vol 178: 160- 174. http://dx.doi.org/10.2307/1541974
37. Lucas H. 1846. "Crustacés, Arachnides, Myriapodes et Hexapodes. Exploration scientifique de l’Algérie pendant les années 1840, 1841, 1842". Sciences physiques, Zoologie 2. Histoire Naturelle des Animaux Articulés Vol 1: 1- 403, plates 1-8.
38. Macia A. 2004. "Primary carbon sources for juvenile penaeid shrimps in a Mangrove-Fringed Bay of Inhaca Island, Mozambique: a dual carbon and nitrogen isotope analysis". Journal Marine Science Vol 3: 151- 161.
39. Milne Edwards H. , 1837. Histoire naturelle des Crustacés, comprenant l’anatomie, la physiologie et la classification de ces animaux. Vol Vol. 2. Librairie Encyclopédique de Roret, Paris.
40. Molina-Poveda C. , Escobar V. , Gamboa-Delgado J. , Cadena E. , Orellana F. , Piña P. . 2002. "Estrategia de alimentación de acuerdo a la demanda fisiológica del juvenil de Litopenaeus vannamei (Boone)", pp.  98- 113. In, Cruz-Suarez L. E. , Rique M. D. , Nieto López M. G. , Simoes N. , Gaxiola G. , Avances en Nutrición Acuícola VI. Memorias del VI Simposio Internacional de Nutrición Acuícola, Cancún, Quintana Roo, del 3 al 6 de septiembre. Universidad Autónoma de Nuevo León, México.
41. Pech D. , Ardisson P. L. , Hernandez-Guevara N. . 2007. "Benthic community response to habitat variation: a case of study from a natural protected area, the Celestun coastal lagoon". Continental Shellfish Research Vol 27: 2523- 2533. http://dx.doi.org/10.1016/j.csr.2007.06.017
42. Perez-Castañeda R. , Defeo O. . 2001. "Population variability of four sympatric penaeid shrimps ( Farfantepenaeusspp.) in a tropical coastal lagoon of Mexico". Estuary Coastal Shellfish Science Vol 52: 631- 641. http://dx.doi.org/10.1006/ecss.2001.0771
43. Perez-Farfante I. 1970. Diagnostic characters of juveniles of the shrimps Penaeus aztecus aztecus, P. duorarum duorarumand P. brasiliensis(Crustacea, Decapoda, Penaeidae). Special Scientific Report of Fisheries, U. S. Fish Wildlife Service, 26 pp.
44. Primavera J. H. 1996. "Stable carbon and nitrogen isotope ratios of penaeid juveniles and primary producers in a riverine mangrove in Guimaras, Philippines". Bulletin of MAJUNE Science Vol 58: 675- 683.
45. Rodriguez A. , Le Vay L. , Mourente G. , Jones D. A. . 1994. "Biochemical composition nd digestive enzyme activity in larvae and postlarvae of Penaeus japonicusduring herbivorous and carnivorous feeding". Marine Biology Vol 118: 45- 51. http://dx.doi.org/10.1007/BF00699218
46. Sainz J. C. , García C. F. , Sierra B. A. , Hernandez C. P. . 2004. "Trypsin syntesis and storage as zymogen in the midgut gland of the shrimp Litopenaeus vannamei ". Journal of Crustacean Biology Vol 24: 266- 273. http://dx.doi.org/10.1651/C-2423
47. Sanchez A. J. 1997. "Habitat preference of Penaeus duorarumBurkenroad (Crustacea: Decapoda) in a tropical coastal lagoon, southwest Gulf of Mexico". Journal of Experimental Marine Biology and Ecology Vol 217: 107- 117. http://dx.doi.org/10.1016/S0022-0981(97)00049-X
48. Sanchez A. J. , Barbaro J. , Delgado G. , Perez L. . 2002. "Evaluación del alimento natural epibentónico en estanques de tierra teniendo en cuenta la densidad de camarones en cultivo". In, I Congreso Iberoamericano Virtual de Acuicultura: 567- 572.
49. Sanchez-Paz A. , Garcia-Carreño F. , Hernandez-Lopez J. , Muhlia-Almazan A. , Yepiz-Plascencia G. . 2007. "Effect of short-term starvation on hepatopancreas and plasma energy reserves of the Pacific white shrimp ( Litopenaeus vannamei)". Journal of Experimental Marine Biology and Ecology Vol 340: 184- 193. http://dx.doi.org/10.1016/j.jembe.2006.09.006
50. Sanchez-Paz A. , Garcia-Carreño F. , Muhlia-Almazan A. , Hernandez-Saavedra N. Y. , Yepiz-Plascencia G. . 2003. "Differential expression of trypsin mRNA in the white shrimp ( Penaeus vannamei) midgut gland under starvation conditions". Journal of Experimental Marine Biology and Ecology Vol 292: 1- 17. http://dx.doi.org/10.1016/S0022-0981(03)00142-4
51. Schwamborn R. , Criales M. . 2000. "Feeding strategy and daily ration of juvenile pink shrimp ( Farfantepenaeus duorarum) in a South Florida seagrass bed". Marine Biology Vol 137: 139- 147. http://dx.doi.org/10.1007/s002270000317
52. Simões S. M. , Costa R. C. , Fransozo A. , Castilho A. L. . 2010. "Diel variation in abundance and size of the seabob shrimp Xiphopenaeus kroyeri(Crustacea, Penaeoidea) in the Ubatuba region, Southeastern Brazil". Anais da Academia Brasileira de Ciências Vol 82: 369- 378. http://dx.doi.org/10.1590/S0001-37652010000200013
53. Stimpson W. 1871. "Notes of Norther American Crustacea in the museum of the Smithsonian Institution. No. III". Annals of the New York Lyceum Vol 10: 92- 136. http://dx.doi.org/10.1111/j.1749-6632.1874.tb00028.x
54. Tapia-Gonzalez F. , Herrera-Silveira J. A. , Aguirre-Macedo M. . 2008. "Water quality variability and eutrophic trends in karstic tropical coastal lagoons of the Yucatan Peninsula". Estuarine, Coastal and Shelf Science Vol 76: 418- 430. http://dx.doi.org/10.1016/j.ecss.2007.07.025
55. van Wormhoudt A. 1980. "Regulation d’activité de l’α amylase à différentes températures d’adaptation et en fonction de l’ablation des pédoncules oculaires et du stade de mue chez Palaemon serratus ". Biochemistry Systemathic Ecology Vol 8: 193- 203. http://dx.doi.org/10.1016/0305-1978(80)90012-5
56. van Wormhoudt A. , Sellos D. . 2003. "Highly variable polymorphism of the alpha- amylase gene family in Litopenaeus vannamei(Crustacea Decapoda)". Journal of Molecular Evolution Vol 57( 6): 659- 671. http://dx.doi.org/10.1007/s00239-003-2516-4
57. van Wormhoudt A. , Le Moullac G. , Klein B. , Sellos D. . 1998. "Caracterización de las tripsinas y amilasas de Penaeus vannamei (Crustacea Decapoda): adaptación a la composición del régimen alimenticio."In, Cruz-Suarez L. E. , Rique M. D. , Nieto López M. G. , Civera R. (eds.), Avances en Nutrición Acuícola. III Memorias del III Simposio Internacional de Nutrición Acuícola, La Paz, Baja California Sur, del 3 al 6 de noviembre. Universidad Autónoma de Nuevo León, México.
58. Vega-Villasante F. , Nolasco-Soria H. , Civera-Cerecedo R. , Gonzalez-Valdes R. , Oliva-Suarez M. . 2000. "Alternativa para la alimentación del Camarón en cultivo: el manejo de la muda", pp.  313- 320. In, Cruz-Suarez L. E. , Rique M. D. , Nieto Lopez M. G. , Olvera M. (eds.), Avances en Nutrición Acuícola. V Memorias del V Simposio Internacional de Nutrición Acuícola, Telchac, Yucatán, del 3 al 6 de noviembre. Universidad Autónoma de Nuevo León, México.
59. Wabete N. 2006. Etude écophysiologique du métabolisme respiratoire et nutritionnel chez la crevette Litopenaeus stylirostris. Application à la crevetticulture en Nouvelle Calédonie. Thèse, Univ. Bordeaux I, Ecole Doctoral Science Vivants, Géoscience Science Environmental, 173 pp.
60. Zar J. 1996. Biostatistical Analysis. Prentice Hall, New York, 663pp.
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