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image of Journal of Crustacean Biology

ABSTRACT DNA content and BrdUrd incorporation were compared to the area of the nucleus of epidermal cells of larval Artemia to test the suitability of nuclear area as a criterion for distinguishing the cell cycle phases in developing tissues in situ. Nuclei in cells in G1 increased in area without increase in DNA content. During the transition from G1 through S to G2, the nuclear area increased as the DNA content doubled. The nuclear area of S phase cells which had incorporated BrdUrd was also determined by measuring the quenched fluorescence of bisbenzimide or immunochemically with a monoclonal antibody to BrdUrd. With both methods, the transition from G1 to S phase could be predicted from the area of the nucleus. Initiation of G2 phase was not clearly defined by nuclear area and there was overlap of the nuclear area of cells in late S, G2, and early prophase. The cell cycle phase profiles of cells in the anteroventral, posteroventral, and dorsal regions of the developing thorax during the first 2 instars were determined using nuclear area. Each region demonstrated different cell cycle kinetics with the greatest amount of proliferation occurring in the region of the developing limb buds in the first segment. The posterior region of the ventral and dorsal surfaces contained cells that demonstrated slower cell cycle transit times as well as cells in G2 that did not proliferate.


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