Cookies Policy
X

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

CELL CYCLE KINETICS OF EPIDERMAL CELLS IN DEVELOPING ARTEMIA: IN SITU ANALYSIS

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Journal of Crustacean Biology

ABSTRACT DNA content and BrdUrd incorporation were compared to the area of the nucleus of epidermal cells of larval Artemia to test the suitability of nuclear area as a criterion for distinguishing the cell cycle phases in developing tissues in situ. Nuclei in cells in G1 increased in area without increase in DNA content. During the transition from G1 through S to G2, the nuclear area increased as the DNA content doubled. The nuclear area of S phase cells which had incorporated BrdUrd was also determined by measuring the quenched fluorescence of bisbenzimide or immunochemically with a monoclonal antibody to BrdUrd. With both methods, the transition from G1 to S phase could be predicted from the area of the nucleus. Initiation of G2 phase was not clearly defined by nuclear area and there was overlap of the nuclear area of cells in late S, G2, and early prophase. The cell cycle phase profiles of cells in the anteroventral, posteroventral, and dorsal regions of the developing thorax during the first 2 instars were determined using nuclear area. Each region demonstrated different cell cycle kinetics with the greatest amount of proliferation occurring in the region of the developing limb buds in the first segment. The posterior region of the ventral and dorsal surfaces contained cells that demonstrated slower cell cycle transit times as well as cells in G2 that did not proliferate.

Loading

Full text loading...

/content/journals/10.1163/193724089x00016
Loading

Data & Media loading...

http://brill.metastore.ingenta.com/content/journals/10.1163/193724089x00016
Loading

Article metrics loading...

/content/journals/10.1163/193724089x00016
1989-01-01
2016-12-03

Sign-in

Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
     
    Journal of Crustacean Biology — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation