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DEVELOPMENT OF PRIMARY CELL CULTURES FROM THE PENAEID SHRIMPS PENAEUS VANNAMEI AND P. INDICUS

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image of Journal of Crustacean Biology

ABSTRACT Tissues and dissociated cells from various organs of the shrimp Penaeus vannamei, as well as embryos of P. indicus, were cultured in different media. Cell outgrowths were observed with ovaries, epidermal regeneration buds, and embryos. Optimal results, i.e., a survival for several months, were obtained in M 199-based medium for ovaries and embryos, and in Grace's medium for regeneration buds. In every case, a 10% fetal bovine serum supplement was needed.

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/content/journals/10.1163/193724096x00711
1996-01-01
2016-12-11

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