Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Digestive proteases from wild and farmed male morphotypes of the Amazon river prawn (Macrobrachium amazonicum)

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

This Article is currently unavailable for purchase.
Add to Favorites
You must be logged in to use this functionality

The aim of this study was to characterize digestive proteases from wild and farmed morphotypes in males of Macrobrachium amazonicum (Heller, 1862). The hepatopancreas of wild and earthen pond reared specimens were collected and homogenized. Unspecific activities of trypsin, chymotrypsin and leucine aminopeptidase were determined using specific substrates. Serine-protease, trypsin, and chymotrypsin specific inhibitors were employed to assess enzyme inhibition. Optimum pH, temperature and thermal stability of the enzymes were also evaluated. Complementary study was carried out using electrophoresis and zymograms. Proteolytic activities were higher in farmed than in the wild prawns and differences were observed among the farmed male morphotypes, while no differences were observed in the wild ones. Both farmed and wild specimens showed similar values of amylase:protease ratio ranging from 0.06 to 0.12. Inhibition assays revealed the presence of serine proteases, trypsin, and chymotrypsin in the hepatopancreas. However, stronger trypsin inhibitions occurred in farmed morphotypes. The optimum temperature and pH were 65°C and 8.0 to 8.5, respectively. Electrophoresis revealed bands between 6 kDa and 205 kDa. Zymograms revealed bands with proteolytic activity. Using the wild animals as a reference, the results suggest that digestive proteases profile changes in farmed prawns, which may be an adaptation to the culture conditions.

Affiliations: 1: 1Laboratório de Enzimologia (LABENZ), Departamento de Bioquímica and Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco-UFPE, Recife, PE, Brazil; 2: 2Universidade Federal do Rio Grande do Norte, Unidade Acadêmica Especializada em Ciências Agrárias, Escola Agrícola de Jundiaí. Av. Jundiai Km 3, CEP 59280-000, Natal, RN, Brazil; 3: 3São Paulo State University (UNESP), Experimental do Litoral Paulista, Praça Infante, 19 Dom Henrique, s/n 11330-900, São Vicente, SP, Brazil


Full text loading...


Data & Media loading...

1. Alcock A. 1905. "A revision of the “genus” Peneus, with diagnoses of some new species and varieties". Annals and Magazine of Natural History Vol 16: 508- 532.
2. Bate C. S. 1881. "On the Penaeidea". Annals and Magazine of Natural History Vol 8: 169- 196.
3. Bate C. S. . 1888. Report on the Crustacea Macrura Collected by H.M.S. Challenger During the Years 1873-76. Report on the Scientific Results of the Voyage of H.M.S. Challenger During the Years 1873-76 Under the Command of Captain George S. Nares, R.N., F.R.S. and the Late Captain Frank Tourle Thomson, R.N. Vol Vol. Zoology – XXIV. Neill and Company, Edinburgh.
4. Bernfeld P. 1955. "Amylases, α and β", pp.  149- 158. In, Colowick S. P. , Kaplan N. O. (eds.), Methods in Enzymology. Academic Press, New York, NY.
5. Bezerra R. S. , Lins E. J. F. , Alencar R. B. , Paiva P. M. G. , Chaves M. E. C. , Coelho L. C. B. B. , Carvalho L. B. Júnior . 2005. "Alkaline proteases from intestine of Nile tilapia ( Oreochromis niloticus)". Process Biochememistry Vol 40: 1829- 1834.
6. Bialetzki A. , Nakatani K. , Baumgartner G. , Bond-Buckup G. . 1997. "Occurrence of Macrobrachium amazonicum(Heller) (Decapoda, Palaemonidae) in Leopoldo’s inlet (Ressaco do Leopoldo), upper Paraná River, Porto Rico, Paraná, Brazil". Revista Brasileira de Zoologia Vol 14: 379- 390.
7. Biesiot P. M. , MacDowell-Capuzzo J. . 1990. "Digestive protease, lipase and amylase activities in stage I larvae of the American lobster Homarus americanus ". Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology Vol 95: 47- 54.
8. Boone L. 1931. "A collection of anomuran and macruran Crustacea from the Bay of Panama and the fresh waters of the Canal Zone". Bulletin of the American Museum of Natural History Vol 63( 2): 137- 189.
9. Bradford M. M. 1976. "A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein binding". Analytical Biochemistry Vol 72: 248- 254.
10. Brito R. , Rosas C. , Chimal M. E. , Gaxiola G. . 2001. "Effect of different diets on growth and digestive enzyme activity in Litopenaeus vannamei(Boone, 1931) early postlarvae". Aquaculture Research Vol 32: 257- 266.
11. Buarque D. S. , Castro P. F. , Santos F. M. S. , Amaral I. P. G. , Oliveira S. M. , Alves K. B. , Carvalho L. B. Jr. , Bezerra R. S. . 2010. "Digestive proteinases and peptidases in the hepatopancreas of the southern brown shrimp ( Farfantepenaeus subtilis) in two sub-adult stages". Aquaculture Nutrition Vol 16: 359- 369.
12. Buarque D. S. , Castro P. F. , Santos F. M. S. , Lemos D. , Carvalho L. B. Jr. , Bezerra R. S. . 2009. "Digestive peptidases and proteinases in the midgut gland of the pink shrimp Farfantepenaeus paulensis(Crustacea, Decapoda, Penaeidae)". Aquaculture Research Vol 40: 861- 870.
13. Burkenroad M. D. 1936. "A new species of Penaeusfrom the American Atlantic". Anais de Academia Brasileira de Ciencias, Rio de Janiero Vol 8: 315- 318.
14. Castro P. F. , Freitas A. C. V. Jr. , Santana W. M. , Carvalho L. B. Jr. , Bezerra R. S. . 2012. "Comparative study of amylases from the midgut gland of three species of penaeid shrimp". Journal of Crustacean Biology Vol 32: 607- 613.
15. Ceccaldi H. J. 1997. "Anatomy and physiology of the digestive system", pp.  261- 291. In, D’Abramo L. R. , Conklin D. E. , Akiyama D. M. (eds.), Crustacean Nutrition Advances in World Aquaculture 6. The World Aquaculture Society, Baton Rouge, LA.
16. Davant P. 1963. Clave para la identificación de los camarones marinos y de río com importância económica en el oriente de Venezuela. Instituto Oceanografico/Universidade de Oriente, Cumana.
17. De Man J. G. 1879. "On some species of the genus PalaemonFabr. with descriptions of two new forms". Notes from the Leyden Museum Vol 41: 165- 184.
18. Fabricius J. C. 1798. Supplementum Entomologiae Systematicae. Hafniae.
19. Galgani F. , Nagayama F. . 1987. "Digestive proteinases in the japanese spiny lobster Panulirus japonieus ". Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Vol 87: 889- 893.
20. Galgani M. L. , Benyamin Y. , Van Wormhoudt A. . 1985. "Purification, properties and immunoassays of trypsin from the shrimp Penaeus japonicus ". Comparative Biochemistry and Physiology Part B Vol 81: 447- 452.
21. García-Carreño F. L. 1992. "The digestive proteases of langostilla ( Pleuroncodes planipes, Decapoda): their partial characterization and the effect of feed on their composition". Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Vol 103: 575- 578.
22. García-Carreño F. L. , Dimes L. E. , Haard N. F. . 1993. "Substrate-gel electrophoresis for composition and molecular weight of proteinases or proteinaceous proteinase inhibitors". Analytical Biochemistry Vol 214: 65- 69.
23. García-Carreño F. L. , Hernández-Cortés M. P. , Haard N. F. . 1994. "Enzymes with peptidase and proteinase activity from the digestive systems of a freshwater and a marine decapod". Journal of Agricultural and Food Chemistry Vol 42: 1456- 1461.
24. Gaxiola G. , Cuzon G. , García T. , Taboada G. , Brito R. , Chimal M. E. , Paredes A. , Soto L. , Rosas C. , Wormhoudt A. V. . 2005. "Factorial effects of salinity, dietary carbohydrate and moult cycle on digestive carbohydrases and hexokinases in Litopenaeus vannamei(Boone, 1931)". Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology Vol 140: 29- 39.
25. Heller C. 1862. "Beiträge zur näheren Kenntnis der Macruren". Sitzungsberichte der mathematisch-natuurwissenschaftlichen Classe der kairserliche Akademie der Wissenschaften, Wien. Vol 45: 389- 426.
26. Hidalgo M. C. , Urea E. , Sanz A. . 1999. "Comparative study of digestive enzymes in fish with different nutritional habits. Proteolytic and amylase activities". Aquaculture Vol 170: 267- 283.
27. Holmes S. P. 1900. "Synopsis of California stalked-eyed Crustacea". Occassional Papers of the California Academy of Sciences Vol 7: 1- 262.
28. Jiang S. T. , Moody M. W. , Chen H. C. . 1991. "Purification and characterization of proteases from digestive tract of grass shrimp ( Penaeus monodon)". Journal of Food Science Vol 56: 322- 326.
29. Johnston D. , Freeman J. . 2005. "Dietary preference and digestive enzyme activities as indicators of trophic resource utilization by six species of crabs". The Biological Bulletin Vol 208: 36- 46.
30. Kim H. R. , Meyers S. P. , Godber J. S. . 1992. "Purification and characterization of anionic trypsins from the hepatopancreas of crayfish, Procambarus clarkii ". Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Vol 103: 391- 398.
31. Kirkwood T. B. L. , Kapahi P. , Shanley D. P. . 2000. "Evolution, stress, and longevity". Journal of Anatomy Vol 197: 587- 590.
32. Kutty M. N. 2005. "Towards sustainable freshwater prawn aquaculture – lessons from shrimp farming, with special reference to India". Aquaculture Research Vol 36: 255- 263.
33. Laemmli U. K. 1970. "Cleavage of structural proteins during the assembly of the head of bacteriophage T4". Nature Vol 227: 680- 685.
34. López-López S. , Nolasco H. , Villarreal-Colmenares H. , Civera-Cerecedo R. . 2005. "Digestive enzyme response to supplemental ingredients in practical diets for juvenile freshwater crayfish Cherax quadricarinatus ". Aquaculture Nutrition Vol 11: 79- 85.
35. Maciel C. R. , Valenti W. C. . 2009. "Biology, Fisheries, and Aquaculture of the Amazon River Prawn Macrobrachium amazonicum: A Review". Nauplius Vol 17: 61- 79.
36. Marques H. L. de A. , Moraes-Valenti P. M. C. . 2012. "Current status and prospects of farming the giant river prawn ( Macrobrachium rosenbergii(De Man, 1879) and the Amazon river prawn Macrobrachium amazonicum(Heller, 1862)) in Brazil". Aquaculture Research Vol 43: 984- 992.
37. Milne Edwards H. 1837. Histoire naturelle des Crustacés, comprenant l’anatomie, la physiologie et la classification de ces animaux. Librairie Encyclopédique de Roret. Vol Vol. 2. Roret, Paris.
38. Moraes-Riodades P. M. C. , Valenti W. C. . 2004. "Morphotypes in male Amazon river prawn Macrobrachium amazonicum ". Aquaculture Vol 236: 297- 307.
39. Moraes-Valenti P. , Valenti W. C. . 2010. "Culture of the Amazon river prawn Macrobrachium amazonicum ", pp.  485- 501. In, New M. B. , Valenti W. C. , Tidwell J. H. , D’Abramo L. R. , Kutty M. N. (eds.), Freshwater Prawns: Biology and Farming. Wiley-Blackwell, Oxford.
40. Moss S. M. , Divakaran S. , Kim B. G. . 2001. "Stimulating effects of pond water on digestive enzyme activity in the Pacific white shrimp, Litopenaeus vannamei(Boone)". Aquaculture Research Vol 32: 125- 131.
41. New M. B. 2005. "Freshwater prawn farming: global status, recent research and a glance at the future". Aquaculture Research Vol 36: 210- 230.
42. New M. B. , Singholka S. , Kutty M. N. . 2000. "Prawn capture fisheries and enhancement", pp.  411- 428. In, New M. B. , Valenti W. C. (eds.), Freshwater Prawn Culture: The Farming of Macrobrachium rosenbergii . Blackwell Science, Oxford.
43. Nunes A. J. P. , Parsons G. J. . 2000. "Effects of the Southern brown shrimp, Penaeus subtilis, predation and artificial feeding on the population dynamics of benthic polychaetes in tropical pond enclosures". Aquaculture Vol 183: 125- 147.
44. Odinetz-Collart O. 1987. "La pêche crevettière de Macrobrachium amazonicum(Palaemonidae) dans le Bas-Tocantins après la fermeture du barrage de Tucurui". Revue d’Hydrobiologie Tropicale Vol 20: 131- 144.
45. Odinetz-Collart O. , Moreira L. C. . 1993. "Potencial pesqueiro do camarão Macrobrachium amazonicumna Amazônia Central (Ilha do Careiro)". Amazoniana Vol 12: 399- 413.
46. Pérez-Farfante I. 1967. "A new species and wo new subspecies of shrimp of the genus Penaeusfrom the wqestern Atlantic". Proceedings of the Biological Society of Washington Vol 80: 83- 100.
47. Pettovelo A. D. 1996. "First record of Macrobrachium amazonicum(Decapoda, Palaemonidae) in Argentina". Crustaceana Vol 69: 113- 114.
48. Sainz J. C. , Garcia-Carreño F. L. , Hernández-Cortés P. . 2004. " Penaeus vannameiisotrypsins: purification and characterization". Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol 138: 155- 162.
49. Santos J. A. , Sampaio C. M. S. , Soares Filho A. A. . 2006. "Male population structure of the amazon river prawn ( Macrobrachium amazonicum) in a natural environment". Nauplius Vol 14: 55- 63.
50. Silva G. M. F. , Ferreira M. A. P. , Von Ledebur E. I. C. F. , Rocha R. M. . 2009. "Gonadal structure analysis of Macrobrachium amazonicum(Heller, 1862) from a wild population: a new insight on the morphotype characterization". Aquaculture Research Vol 40: 798- 803.
51. Solorzano L. 1969. "Determination of ammonia in natural waters by the phenythypochlorite method". Limnology and Oceanography Vol 14: 799- 801.
52. Stimpson W. 1860. "Prodromus desccriptionis animalium evertebratorum, quae in expeditione ad Oceanum Pacificum Septentrionalem, a Republica Federata missa, Cadwaladaro Ringgold et Johanne Rodgers Ducibus, observavit et descripsit. Pars VIII. Crustacea Macrura". Proceedings of the Academy of Natural Sciences of Philadelphia Vol 12: 22- 47.
53. Tsai I. H. , Chuang K. L. , Chuang J. L. . 1986. "Chymotrypsins in digestive tracts of crustacean decapods (shrimps)". Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Vol 85: 235- 240.
54. van Wormhoudt A. , Ceccaldi H. J. , Martin B. J. . 1980. "Adaptation de la teneur en enzymes digestives de l’hepatopancreas de Palaemon serratus(Crustacea, Decapoda) a la composition d’aliments experimentaux". Aquaculture Vol 21: 63- 78.
55. Vega-Villasante F. , Nolasco H. , Civera R. . 1995. "Digestive enzymes of the pacific brown shrimp Penaeus californiensis-II. Properties of protease activity in the whole digestive tract". Comparative Biochemistry and Physiology Part B: Comparative Biochemistry and Molecular Biology Vol 112: 123- 129.
56. Vonk H. J. , Western J. R. H. . 1984. Comparative Biochemistry and Physiology of Enzymatic Digestion. 1st Edition. Academic Press, London.

Article metrics loading...



Can't access your account?
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation