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Abnormalities and possible mosaicism during embryonic cell division after cold shock in zygotes of the Pacific white shrimp, Litopenaeus vannamei, related to failure of induction of tetraploidy and triploidy

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Abnormalities and possible mosaicism during embryonic cell division after cold shock of zygotes of Litopenaeus vannamei (Boone, 1931) were related to the failure of induction of polyploidy. Eggs were treated to 10 minutes cold shock at 8°C to arrest the second polar body (triploid-induced eggs) or first cleavage (tetraploid-induced eggs). In both cases, asynchrony and abnormalities among embryos were observed. In some triploid-induced eggs, three haploid pronuclei, instead of two, were seen at the center of the egg, and none in the periphery. These three nuclei may serve to evaluate methods of triploid induction in penaeids. In tetraploid-induced eggs, a tetrapolar or a bipolar spindle was inferred. The common abnormality in the anaphase with a tetrapolar spindle came with an off-center position of the nucleus, ending in a 2-cell stage with either a blastomere with one complete nucleus (4n) or two separate nuclei (2n and 2n), both with one anucleated (0n) blastomere. Other abnormalities produced 3- and 4-cell stages with one to three anucleated (0n) blastomeres, including one putative mosaic. In triploid-induced eggs, the main abnormalities came with syngami between two pronuclei only, ending in a putative 4-cell stage 1:1 triploid (3n): diploid (2n) mosaic egg or without syngamy, ending in a putative 4-cell stage 2:1:0 diploid 2(2n): haploid (n): anucleated (0n) mosaic egg, and perhaps a putative 1:1 diploid (2n): haploid (n) mosaic egg. Less frequent 4-cell stage arrangements had 1:1 sextaploid (6n): anucleated (0n) and 1:3 one nucleated (3n) and three anucleated (0n) blastomeres. We suggest that inherent problems in L. vannamei embryos are related to lethal occurrences of putative nauplii mosaics. Alternative methods to induce polyploidy are needed for this species.

Affiliations: 1: 1Instituto Tecnológico de Sonora (ITSON), Calle 5 de Febrero 818 Sur, Cd Obregón, Sonora 85000, Mexico; 2: 2Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Calle Instituto Politécnico Nacional # 195, La Paz, Baja California Sur 23096, Mexico; 3: 3Departamento de Investigaciones Científicas y Tecnológicas, Universidad de Sonora, Avenida Colosio s/n, Hermosillo, Sonora 83000, Mexico

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1. Alok D. , Abeydeera L. , Van Der Steen H. . 2006. "Reproductive technologies for genetic improvements and marine shrimp production in controlled environments". World Aquaculture Magazine Vol 37: 50- 59.
2. Bate C. S. 1888. Report on the Crustacea Macrura collected by the H.M.S. Challenger during the years 1873-76, 24: i-xc + 1-942, plates 1-150 in separate volume.
3. Biffis C. , Alwes F. , Scholtz G. . 2009. "Cleavage and gastrulation of the dendrobranchiate shrimp Penaeus monodon(Crustacea, Malacostraca, Decapoda)". Arthropod Structure and Development Vol 38: 527- 540. http://dx.doi.org/10.1016/j.asd.2009.06.003
4. Boone L. 1931. "Anomuran, Macruran Crustecea from Panama and Canal Zone". Bulletin of the American Museum of Natural History Vol 63: 137- 189.
5. de Almeida Aloise D. , Maia-Lima F. D. , de Oliveira R. M. , Cabral T. D. , Molina W. F. . 2011. "Ploidy manipulation and polyploidy detection in the white shrimp Litopenaeus vannamei(Boone 1931) (Decapoda, Penaeidae)". Marine Biotechnology Vol 13: 41- 47. http://dx.doi.org/10.1007/s10126-010-9266-2
6. Dumas S. , Campos-Ramos R. . 1999. "Triploidy induction in the Pacific white shrimp Litopenaeus vannamei(Boone)". Aquaculture Research Vol 30: 621- 624. http://dx.doi.org/10.1046/j.1365-2109.1999.00353.x
7. Fabricius J. C. 1798. Supplementum Entomologiae Systematicae, pp. 1-572.
8. Foote A. R. , Mair G. C. , Wood A. T. , Sellars M. J. . 2012. "Tetraploid inductions of Penaeus monodonusing cold shock". Aquaculture International Vol 20: 1003- 1007. http://dx.doi.org/10.1007/s10499-012-9524-6
9. Foote A. R. , Sellars M. J. , Coman G. J. , Merrit D. J. . 2010. "Tetraploar mitotic-spindle and off-centre pronucleus lead to non-viable development in tetraploid Penaeus japonicus(Bate) (Kuruma shrimp)". Arthropod Structure and Development Vol 39: 268- 275. http://dx.doi.org/10.1016/j.asd.2009.12.001
10. Garcina-Rivera C. , Arredondo-Figueroa J. L. , Barriga-Sosa I. A. . 2004. "Optimisation of triploidy induction in the Pacific white shrimp, Litopenaeus vannamei ". Journal of Applied Aquaculture Vol 16: 85- 94. http://dx.doi.org/10.1300/J028v16n01_07
11. Hertzler P. L. 2005a. "Cleavage and gastrulation in the shrimp Penaeus( Litopenaeus) vannamei(Malacostraca, Decapoda, Dendrobranchiata)". Arthropod Structure and Development Vol 34: 455- 469. http://dx.doi.org/10.1016/j.asd.2005.01.009
12. Hertzler P. L. . 2005b. "Timing of meiotic maturation and first mitosis in the Pacific white shrimp Litopenaeus vannamei ". Journal of the World Aquaculture Society Vol 36: 570- 576. http://dx.doi.org/10.1111/j.1749-7345.2005.tb00408.x
13. Li F. , Xiang J. , Zhang X. , Wu C. , Zhang C. , Zhou L. , Yu K. . 2003. "Tetraploid induction by heatshocks in Chinese shrimp Fenneropenaeus chinensis ". Journal of Shellfish Research Vol 22: 541- 545.
14. Milne Edwards H. 1837. Historie naturelle des Crustacés, comprenant l’anatomie, la physiologie et la classification de ces animaux. Vol. 2. Paris: 1-532, Pls. 1-14, 14bis, 15-25, 25bis, 26-42.
15. Morelli M. , and Aquacop. 2003. "Effects of heat-shock on cell division and microtubule organization in zygotes of the shrimp Penaeus indicus(Crustacea, Decapoda) observed with confocal microscopy". Aquaculture Vol 216: 39- 53. http://dx.doi.org/10.1016/S0044-8486(01)00894-8
16. Osbeck P. 1765. Reise nach Ostindien und China. Nebst O. Toreens Reise nach Suratte und C. G. Ekebergs Nachricht von der Landwirthschaft der Chineser. Aus dem Schwedischen übersetzt von J. G. Georgi. Rostock. xxiv + 552 pages, 13 plates.
17. Pongtippatee P. , Laburee K. , Thaweethamsewee P. , Hiranphan R. , Asuvapongpatana S. , Weerachatyanukul W. , Srisawat T. , Withyachumnarnkul B. . 2012. "Triploid Penaeus monodon: sex ratio and growth rate". Aquaculture Vol 356-357: 7- 13. http://dx.doi.org/10.1016/j.aquaculture.2012.06.004
18. Sellars M. J. , Arce S. M. , Hertzler P. L. . 2012. "Triploidy induction in the Pacific white shrimp Litopenaeus vannamei: an assessment of induction agents and parameters, embryo viability, and early larval survival". Marine Biotechnology Vol 14: 740- 751. http://dx.doi.org/10.1007/s10126-012-9439-2
19. Sellars M. J. , Coman F. E. , Degnan B. M. , Preston N. P. . 2006. "The effectiveness of 6-dimethylaminopurine, heat and cold shocks for inducing tetraploidy in the Kuruma prawn, Penaeus( Marsupenaeus) japonicus(Bate)". Journal of Shellfish Research Vol 25: 631- 638. http://dx.doi.org/10.2983/0730-8000(2006)25[631:TEOHCA]2.0.CO;2
20. Sellars M. J. , Li F. , Preston N. P. , Xiang J. . 2010. "Penaeid shrimp polyploidy: global status and future direction". Aquaculture Vol 310: 1- 7. http://dx.doi.org/10.1016/j.aquaculture.2010.10.002
21. Wood A. T. , Coman G. J. , Foote A. R. , Sellars M. J. . 2011. "Triploid induction of black tiger shrimp, Penaeus monodon(Fabricius) using cold shock". Aquaculture Research Vol 42: 1741- 1744. http://dx.doi.org/10.1111/j.1365-2109.2010.02763.x
22. Zhang X. , Asami T. , Onozato H. . 2007. "Polypolar spindle formation during first cell cycle in rainbow trout Oncorhynchus mykissembryos after heat-shock treatment". Fisheries Science Vol 73: 1325- 1331. http://dx.doi.org/10.1111/j.1444-2906.2007.01425.x
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2014-05-07
2016-12-07

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