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Characterization of twelve new microsatellite markers for the endangered Chinese horseshoe crab ( Tachypleus tridentatus) through next-generation sequencing

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The Chinese horseshoe crab, Tachypleus tridentatus(Leach, 1819), is an endangered marine benthic arthropod. In this study, we describe and characterize twelve new microsatellite markers for T. tridentatusthrough mining next-generation sequencing data. Genotypes from two populations, Beihai ( n = 15 ) and Dongshan ( n = 15 ), demonstrated the usefulness of these loci for population-level studies. The number of alleles ranged from 3 to 8, with an average of 4.67 alleles per locus. The observed and expected heterozygosity in our populations ranged from 0.0667 to 0.9444, and 0.2339 to 0.8253, respectively. These new informative microsatellite markers will be useful for further genetic variation analyses, parentage determination and conservation studies.

Affiliations: 1: 3Key Laboratory for Ecological Environment in Coastal Areas (SOA), National Marine Environmental Monitoring Center, Dalian 116023, P.R. China


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