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An anti-lipopolysaccharide factor in Litopenaeus vannameiparticipates in the immune defense against WSSV and Vibrio anguillarum

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The anti-lipopolysaccharide factors (ALFs) are a group of effector molecules of innate immunity in arthropods, exhibiting binding and neutralizing activities to lipopolysaccharides. In the present paper, an ALF gene from shrimp Litopenaeus vannamei(Boone, 1931) ( LvALF) was cloned by rapid amplification of cDNA ends (RACE) technique, which encodes a polypeptide of 122 amino acids with a predicted molecular mass of 13.7 kDa and a theoretical isoelectric point of 9.95. In healthy shrimp, the LvALF mRNA was highly expressed in hemocytes, and moderately in gill and heart, whereas low in muscle, intestine and hepatopancres. While the healthy shrimps were challenged with white spot syndrome virus (WSSV) and Vibrio anguillarum, the LvALF mRNA level in hemocytes was significantly up-regulated and reached peak levels at 6-12 h and then gradually decreased to the control level. Moreover, the LPS-binding activity of recombinant LvALF (r LvALF) was confirmed by ELISA and modified pull-down assay using the polyclonal antibodies against the r LvALF, meanwhile the up-regulated expressions of LvALF in hemocytes after challenges with WSSV or V. anguillarumwere also detected at protein and cellular levels by Western blotting and immunocytochemistry. These results indicated that LvALF was involved in the immune defense against WSSV and V. anguillarum.

Affiliations: 1: Laboratory of Pathology and Immunology of Aquatic Animals, Ocean University of China, 5 Yushan Road, Qingdao 266003, P. R. China

10.1163/1937240X-00002364
/content/journals/10.1163/1937240x-00002364
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1. Aketagawa J. , Miyata T. , Ohtsubo S. , Nakamura T. , Morita T. , Hayashida H. , Miyata T. , Iwanaga S. , Takao T. , Shimonishi Y. . 1986. "Primary structure of limulusanticoagulant anti-lipopolysaccharide factor". Journal of Biological Chemistry Vol 261: 7357- 7365.
2. Amparyup P. , Kondo H. , Hirono I. , Aoki T. , Tassanakajon A. . 2008. "Molecular cloning, genomic organization and recombinant expression of a crustin-like antimicrobial peptide from black tiger shrimp Penaeus monodon ". Molecular Immunology Vol 45: 1085- 1093. http://dx.doi.org/10.1016/j.molimm.2007.07.031
3. Bachere E. , Mialhe E. , Noel D. , Boulo V. , Morvan A. , Rodriguez J. . 1995. "Knowledge and research prospects in marine mollusc and crustacean immunology". Aquaculture Vol 132: 17- 32. http://dx.doi.org/10.1016/0044-8486(94)00389-6
4. Bangrak P. , Graidist P. , Chotigeat W. , Supamattaya K. , Phongdara A. . 2002. "A syntenin-like protein with postsynaptic density protein (PDZ) domains produced by black tiger shrimp Penaeus monodonin response to white spot syndrome virus infection". Diseases of Aquatic Organisms Vol 49: 19- 25. http://dx.doi.org/10.3354/dao049019
5. Bate C. S. 1888. Report on the Crustacea Macrura collected by H.M.S. Challenger during the Years 1873-76. In, J. Murray (ed.), Zoology. Report on the Scientific Results of the Voyage of H.M.S. Challenger During the Years 1873-76 Under the Command of Captain George S. Nares, R.N., F.R.S. and the Late Captain Frank Tourle Thomson, R.N. Wyville Thomson, C. and J. Murray (series eds.) Vol. 24. Neill and Company, Edinburgh, I-xc, 1-942 pp.
6. Boone L. 1931. "A collection of anomuran and macruran Crustacea from the Bay of Panama and the fresh waters of the Canal Zone". Bulletin of the American Museum of Natural History Vol 63: 137- 189.
7. Dana J. D. 1852. "Conspectus Crustaceorum &c. Conspectus of the Crustacea of the exploring expedition under Capt. Wilkes, U.S.N., including the Crustacea Cancroidea Corystoidea". Proceedings of the Academy of Natural Sciences of Philadelphia Vol 6: 73- 86.
8. de-la-Re-Vega E. , García-Galaz A. , Díaz-Cinco M. E. , Sotelo-Mundo R. R. . 2006. "White shrimp ( Litopenaeus vannamei) recombinant lysozyme has antibacterial activity against Gram negative bacteria: Vibrio alginolyticus, Vibrio parahemolyticus, and Vibrio cholerae ". Fish and Shellfish Immunology Vol 20: 405- 408. http://dx.doi.org/10.1016/j.fsi.2005.06.005
9. de la Vega E. , O’Leary N. A. , Shockey J. E. , Robalino J. , Payne C. , Browdy C. L. , Warr G. W. , Gross P. S. . 2008. "Anti-lipopolysaccharide factor in Litopenaeus vannamei(LvALF): a broad spectrum antimicrobial peptide essential for shrimp immunity against bacterial and fungal infection". Molecular Immunology Vol 45: 1916- 1925. http://dx.doi.org/10.1016/j.molimm.2007.10.039
10. Dhar A. K. , Dettori A. , Roux M. M. , Klimpel K. R. , Read B. . 2003. "Identification of differentially expressed genes in shrimp ( Penaeus stylirostris) infected with white spot syndrome virus by cDNA microarrays". Archives of Virology Vol 148: 2381- 2396. http://dx.doi.org/10.1007/s00705-003-0172-z
11. Fabricius J. C. 1798. Supplementum Entomologiae Systematicae. Hafniae.
12. Gross P. S. , Bartlett T. C. , Browdy C. L. , Chapman R. W. , Warr G. W. . 2001. "Immune gene discovery by expressed sequence tag analysis of hemocytes and hepatopancreas in the Pacific white shrimp, Litopenaeus vannamei, and the Atlantic white shrimp, L. setiferus ". Developmental and Comparative Immunology Vol 25: 565- 577. http://dx.doi.org/10.1016/S0145-305X(01)00018-0
13. He N. , Qin Q. W. , Xu X. . 2005. "Differential profile of genes expressed in hemocytes of White Spot Syndrome Virus-resistant shrimp ( Penaeus japonicus) by combining suppression subtractive hybridization and differential hybridization". Antiviral Research Vol 66: 39- 45. http://dx.doi.org/10.1016/j.antiviral.2004.12.010
14. Imjongjirak C. , Amparyup P. , Tassanakajon A. , Sittipraneed S. . 2007. "Antilipopolysaccharide factor (ALF) of mud crab Scylla paramamosain: molecular cloning, genomic organization and the antimicrobial activity of its synthetic LPS binding domain". Molecular Immunology Vol 44: 3195- 3203. http://dx.doi.org/10.1016/j.molimm.2007.01.028
15. Jiravanichpaisal P. , Sricharoen S. , Söderhäll I. , Söderhäll K. . 2006. "White spot syndrome virus interaction with freshwater crayfish". Fish and Shellfish Immunology Vol 20: 718- 727. http://dx.doi.org/10.1016/j.fsi.2005.09.002
16. Leach W. E. 1819. Dictionaire des Sciences Naturelles. 14: 58.
17. Li C. H. , Zhao J. M. , Song L. S. , Mu C. K. , Zhang H. , Gai Y. C. , Qiu L. M. , Yu Y. D. , Ni D. J. , Xing K. Z. . 2008. "Molecular cloning, genomic organization and functional analysis of an anti-lipopolysaccharide factor from Chinese mitten crab Eriocheir sinensis ". Developmental and Comparative Immunology Vol 32: 784- 794. http://dx.doi.org/10.1016/j.dci.2007.11.008
18. Li Q. , Zhan W. B. , Xing J. , Sheng X. Z. . 2007. "Production, characterization and applicability of monoclonal antibodies to immunoglobulin of Japanese flounder ( Paralichthys olivaceus)". Fish and Shellfish Immunology Vol 23: 982- 990. http://dx.doi.org/10.1016/j.fsi.2007.03.008
19. Lightner D. V. , Redman R. M. . 1998. "Shrimp diseases and current diagnostic methods". Aquaculture Vol 164: 201- 220. http://dx.doi.org/10.1016/S0044-8486(98)00187-2
20. Linnaeus C. 1758. Systema Naturae per Regna Tria Naturae, Secundum Classes, Ordines, Genera, Species, cum Characteribus, Differentiis, Synonymis, Locis (edit. 10). Vol Vol. 1. Laurentii Salvii, Holmiae [Stockholm].
21. Linnaeus C. . 1758. Systema Naturae per Regna Tria Naturae, Secundum Classes, Ordines, Genera, Species, cum Characteribus, Differentiis, Synonymis, Locis (edit. 12). Vol. 1. Holmiae [Stockholm].
22. Liu F. S. , Liu Y. C. , Li F. H. , Dong B. , Xiang J. H. . 2005. "Molecular cloning and expression profile of putative antilipopolysaccharide factor in Chinese shrimp ( Fenneropenaeus chinensis)". Marine Biotechnology Vol 7: 600- 608. http://dx.doi.org/10.1007/s10126-005-5006-4
23. Liu H. , Söderhäll K. , Jiravanichpaisal P. . 2009. "Antiviral immunity in crustaceans". Fish and Shellfish Immunology Vol 27: 79- 88. http://dx.doi.org/10.1016/j.fsi.2009.02.009
24. Liu H. P. , Jiravanichpaisal P. , Söderhäll I. , Cerenius L. , Söderhäll K. . 2006. "Antilipopolysaccharide factor interferes with white spot syndrome virus replication in vitro and in vivo in the crayfish Pacifastacus leniusculus ". Journal of Virology Vol 80: 10365- 10371. http://dx.doi.org/10.1128/JVI.01101-06
25. Liu J. W. , Yu Y. , Li F. H. , Zhang X. J. , Xiang J. H. . 2014. "A new anti-lipopolysaccharide factor (ALF) gene with its SNP polymorphisms related to WSSV-resistance of Litopenaeus vannamei ". Fish and Shellfish Immunology Vol 39: 24- 33. http://dx.doi.org/10.1016/j.fsi.2014.04.009
26. Livak K. J. , Schmittgen T. D. . 2001. "Analysis of relative gene expression data using realtime quantitative PCR and the 2 − Δ Δ CT method". Methods Vol 25: 402- 408. http://dx.doi.org/10.1006/meth.2001.1262
27. Loker E. S. , Adema C. M. , Zhang S. M. , Kepler T. B. . 2004. "Invertebrate immune systems – not homogeneous, not simple, not well understood". Immunological Reviews Vol 198: 10- 24. http://dx.doi.org/10.1111/j.0105-2896.2004.0117.x
28. Luo T. , Li F. , Lei K. , Xu X. . 2007. "Genomic organization, promoter characterization and expression profiles of an antiviral gene PmAV from the shrimp Penaeus monodon ". Molecular Immunology Vol 44: 1516- 1523. http://dx.doi.org/10.1016/j.molimm.2006.09.015
29. Luo T. , Zhang X. B. , Shao Z. Z. , Xu X. . 2003. "PmAV, a novel gene involved in virus resistance of shrimp Penaeus monodon ". FEBS Letters Vol 551: 53- 57. http://dx.doi.org/10.1016/S0014-5793(03)00891-3
30. Muta T. , Miyata T. , Tokunaga F. , Nakamura T. , Iwanaga S. . 1987. "Primary structure of anti-lipopolysaccharide factor from American horseshoe crab, Limulus polyphemus ". Journal of Biochemistry Vol 101: 1321- 1330.
31. Nagoshi H. , Inagawa H. , Morii K. , Harada H. , Kohchi C. , Nishizawa T. , Taniquchi Y. , Uenobe M. , Honda T. , Kondoh M. , Takahashi Y. , Soma G. . 2006. "Cloning and characterization of a LPS-regulatory gene having an LPS binding domain in kuruma prawn Marsupenaeus japonicus ". Molecular Immunology Vol 43: 2061- 2069. http://dx.doi.org/10.1016/j.molimm.2005.12.009
32. Osbeck P. 1765. Reise nach Ostindien und China. Nebst O. Toreens nach Suratte und C. G. Ekebergs Nachricht von der Landwirthschaft der Chineser. Aus dem Schwedischen übersetzt von J. G. Georgi. Rostock.
33. Rosa R. D. , Bandeira P. T. , Barracco M. A. . 2007. "Molecular cloning of crustins from the hemocytes of Brazilian penaeid shrimps". FEMS Microbiology Letters Vol 274: 287- 290. http://dx.doi.org/10.1111/j.1574-6968.2007.00866.x
34. Rosa R. D. , Vergnes A. , de Lorgeril J. , Goncalves P. , Perazzolo L. M. , Saune L. , Romestand B. , Fievet J. , Gueguen Y. , Bachere E. , Destoumieux-Garzon D. . 2013. "Functional divergence in shrimp anti-lipopolysaccharide factors (ALFs): from recognition of cell wall components to antimicrobial activity". PLOS One Vol 8: e67937.
35. Rout N. , Citarasu T. , Ravindran R. , Murugan V. . 2005. "Transcriptional and translational expression profile of a white spot syndrome viral (WSSV) gene in different organs of infected shrimp". Aquaculture Vol 245: 31- 38. http://dx.doi.org/10.1016/j.aquaculture.2004.11.040
36. Somboonwiwat K. , Bachere E. , Rimphanitchayakit V. , Tassanakajon A. . 2008. "Localization of anti-lipopolysaccharide factor (ALFPm3) in tissues of the black tiger shrimp, Penaeus monodon, and characterization of its binding properties". Developmental and Comparative Immunology Vol 32: 1170- 1176. http://dx.doi.org/10.1016/j.dci.2008.03.008
37. Somboonwiwat K. , Marcos M. , Tassanakajon A. , Klinbunga S. , Aumelas A. , Romestand B. , Guequen Y. , Boze H. , Moulin G. , Bachere E. . 2005. "Recombinant expression and anti-microbial activity of anti-lipopolysaccharide factor (ALF) from the black tiger shrimp Penaeus monodon ". Developmental and Comparative Immunology Vol 29: 841- 851. http://dx.doi.org/10.1016/j.dci.2005.02.004
38. Stimpson W. 1874. "Notes on North American Crustacea in the museum of the Smithsonian Institution. No. III". Annals of the Lyceum of Natural History of New York Vol 10( 6): 119- 163.
39. Supungul P. , Klinbunga S. , Pichyangkura R. , Hirono I. , Aoki T. , Tassanakajon A. . 2004. "Antimicrobial peptides discovered in the black tiger shrimp Penaeus monodonusing the EST approach". Diseases of Aquatic Organisms Vol 61: 123- 135. http://dx.doi.org/10.3354/dao061123
40. Supungul P. , Tang S. , Maneeruttanarungroj C. , Rimphanitchayakit V. , Hirono I. , Aoki T. , Tassanakajon A. . 2008. "Cloning, expression and antimicrobial activity of crustinPm1, a major isoform of crustin, from the black tiger shrimp Penaeus monodon ". Developmental and Comparative Immunology Vol 32: 61- 70. http://dx.doi.org/10.1016/j.dci.2007.04.004
41. Tanaka S. , Nakamura T. , Morita T. , Iwanaga S. . 1982. "Limulus anti-LPS factor: an anticoagulant which inhibits the endotoxin mediated activation of Limulus coagulation system". Biochemical and Biophysical Research Communications Vol 105: 717- 723. http://dx.doi.org/10.1016/0006-291X(82)91493-0
42. Tang X. Q. , Wang X. L. , Zhan W. B. . 2012. "An integrin b subunit of Chinese shrimp involved in WSSV infection". Aquaculture Vol 368-369: 1- 9. http://dx.doi.org/10.1016/j.aquaculture.2012.08.004
43. Tassanakajon A. , Klinbunga S. , Paunglarp N. , Rimphanitchayakit V. , Udomkit A. , Jitrapakdee S. , Sritunyalucksana K. , Phongdara A. , Pongsomboon S. , Supungul P. , Tang S. , Kuphanumart K. , Pichyangkura R. , Lursinsap C. . 2006. " Penaeus monodongene discovery project: the generation of an EST collection and establishment of a database". Gene Vol 384: 104- 112. http://dx.doi.org/10.1016/j.gene.2006.07.012
44. Tharntada S. , Ponprateep S. , Somboonwiwat K. , Liu H. P. , Soderhall I. , Soderhall K. , Tassanakajon A. . 2009. "Role of anti-lipopolysaccharide factor from the black tiger shrimp, Penaeus monodon, in protection from white spot syndrome virus infection". Journal of General Virology Vol 90: 1491- 1498. http://dx.doi.org/10.1099/vir.0.009621-0
45. Tharntada S. , Somboonwiwat K. , Rimphanitchayakit V. , Tassanakajon A. . 2008. "Anti-lipopolysaccharide factors from the black tiger shrimp, Penaeus monodon, are encoded by two genomic loci". Fish and Shellfish Immunology Vol 24: 46- 54. http://dx.doi.org/10.1016/j.fsi.2007.07.010
46. Tyagi A. , Khushiramani R. , Karunasagar I. , Karunasagar I. . 2007. "Antivibrio activity of recombinant lysozyme expressed from black tiger shrimp, Penaeus monodon ". Aquaculture Vol 272: 246- 253. http://dx.doi.org/10.1016/j.aquaculture.2007.08.055
47. Wang D. N. , Chen L. , Liu J. W. , He Z. Y. , Zhang W. J. , Wu X. F. . 2001. "The native gene of anti-LPS factor from Tachypleus tridentatus: cloning, expression and its bacteriostatic activity in vitro ". Protein and Peptide Letters Vol 8: 273- 280. http://dx.doi.org/10.2174/0929866013409337
48. Yang Y. , Boze H. , Chemardin P. , Padilla A. , Moulin G. , Tassanakajon A. , Puqniere M. , Roquet F. , Destoumieux-Garzon D. , Guequen Y. , Bachere E. , Aumelas A. . 2009. "NMR structure of rALF-Pm3, an anti-lipopolysaccharide factor from shrimp: model of the possible lipid A-binding site". Biopolymers Vol 91: 207- 220. http://dx.doi.org/10.1002/bip.21119
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