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ABSTRACT The diploid chromosome number 2n = 24 has been established in the harpacticoid copepod Tigriopus sp. coming from Kerguelen Island, while the population of Tigriopus inhabiting Crozet Island possesses the diploid number 2n = 22. The two populations differ also in homogeneity of the karyotype, thereby further supporting the hypothesis that these two populations belong to different species.