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CLONING AND SCREENING OF DNA PROBES FOR GENETIC STUDIES IN STONE CRABS (DECAPODA: XANTHIDAE: MENIPPE)

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ABSTRACT A recombinant library was constructed from genomic DNA of Menippe adina and characterized by Southern blot analysis. Cesium chloride/ethidium bromide equilibrium centrifugation of testes lysates yielded high molecular weight DNA of sufficient purity for restriction endonuclease digestion. The library was constructed in the plasmid pUC 19 from genomic DNA of Menippe that had been partially digested with the restriction endonuclease Sau3A I. A Southern blot of nine randomly chosen clones from this library was hybridized with total genomic DNA to assess relative abundances of these sequences in the genomes of M. adina and M. mercenaria. These assessments were consistent with the results of genomic Southern blot analysis in which specific clones were used as hybridization probes on blots of total genomic DNA. Potential applications of these cloned sequences as hybridization probes for evolutionary genetic studies are discussed.

10.1163/1937240X91X00347
/content/journals/10.1163/1937240x91x00347
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/content/journals/10.1163/1937240x91x00347
2017-08-20

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