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Investigations of Internal Interactions between the Parasitic Barnacle Loxothylacus Texanus (Rhizocephala: Sacculinidae) and Its Host Callinectes Sapidus (Brachyura: Portunidae) Using PCR Techniques

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AbstractWe describe techniques that enable the preservation of tissues from the rhizocephalan barnacle, Loxothylacus texanus, inside the body cavities of blue crab hosts, Callinectes sapidus, in a manner that minimizes the degradative activities of hepatopancreatic enzymes. These procedures allow the extraction and amplification of both parasite and host 18S rDNA within the same sample and enable one to distinguish between parasitized and unparasitized crab tissue in as little as two weeks after infection, well before any external manifestations of the parasites. Two PCR-based approaches were taken to identify the presence of L. texanus. In the first approach, a set of primers specific for L. texanus was used to specifically amplify 18S sequence in a background of C. sapidus DNA or the DNA of other barnacle species. In the second approach, a set of general primers was used to amplify 18S sequence from C. sapidus and a variety of barnacle species. The products of this PCR were then digested with an enzyme that recognizes a restriction site present only in the L. texanus PCR product to yield a unique pattern of fragments. With these techniques, we could detect as few as five parasitic cypris larvae in water samples, as well as L. texanus in the tissue of a small crab collected from the field and in the four anterior periopods of a crab bearing the external stage of the parasite. In experiments with potential hosts of varying sizes and molt stages, we confirmed that the parasite was significantly more effective in infecting crabs less than 30 mm carapace width than larger individuals. These techniques will facilitate future investigations of ecological and physiological interactions between these important crustacean parasites and their hosts and will help to determine the economic impact of this parasite on blue crab fisheries.

Affiliations: 1: c(EB) Department of Biology, University of Richmond, Richmond, Virginia 23173, U.S.A ; 2: b(TDS, ABM, AW, DLM, EG, CG, JJOB) Department of Biological Sciences, LSCB Room 124, University of South Alabama, Mobile, Alabama 36688, U.S.A.


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1. Abele L. G. , Spears T. , Kim W. , Applegate M. 1992 "Phylogeny of selected maxillopodan and other crustacean taxa based on 18S ribosomal nucleotide sequences: a preliminary analysis." Acta Zoologica Vol 73 373 382
2. Arrotin P. , Demoulin V. 1993 "A parsimony analysis of eukaryotic small subunit ribosomal RNA (18S) sequences." Biochemical and Systematic Ecology Vol 21 133
3. Barker R. H. 1994 "The use of PCR in the field." Parasitology Today Vol 10 3 117 119
4. Barnes R. S K. , Calow P. , Olive P. J W. 1993 The Invertebrates: a new synthesis. Blackwell Scientific Publications, Boston, Massachusetts. 207 pp
5. Boas J. E V. 1920 "Lehrbuch der Zoologie fr Studierende." Gustav Fischer Verlag, Jena, Germany. 302 pp
6. Boone E. J. , Boettcher A. A. , Sherman T. D. , O'Brien J. J. 2003 "Characterization of settlement cues used by the rhizocephalan barnacle, Loxothylacus texanus." Marine Ecology Progress Series Vol 252 187 197
7. Boone E. J. , Boettcher A. A. , Sherman T. D. , O'Brien J. J. 2004 "What constrains the geographic and host range of the rhizocephalan, Loxothylacus texanus in the wild?." Journal of Marine Biology and Ecology Vol 309 129 139
8. Boschma H. 1933 "The Rizocephala in the collections of the British Museum." Jourbal of the Linnean Society, London (Zoology) Vol 38 473 552
9. Christmas J. Y. 1969 "Parasitic barnacles in Mississippi estuaries with special reference to Loxothylacus texanus Boschma in the blue crab (Callinectes sapidus)." Proceedings of the 22nd Annual Conference of the Southeastern Association of the Game and Fish Commission. pp. 272-275
10. Darwin C. 1854 "A monograph of the subclass Cirripedia, with figures of all the species. The Balanidae, the Verucidae, etc." Ray Society, London
11. Gannon A. T. 1990 "Distribution of Octolasmis mlleri, an ectocommensal gill barnacle, on the blue crab." Bulletin of Marine Science Vol 46 55 61
12. Giard A. 1886 "De i'influence de certains parasites Rhizocphales sur les caractres; sexuels extrieurs de leur hôte." Comptes Rendus Hebdomadaires des Sances des l'Acadmie des Sciences de Paris Vol 103 84 86
13. Glenner H. 2001 "Cypris metamorphosis, injection and earliest internal development of the kentrogonid rhizocephalan Loxothylacus panopaei." Journal of Morphology Vol 249 43 75
14. Glenner H. , Hebsgaard M. B. 2006 "Phylogeny and evolution of life history strategies of the parasitic barnacles (Crustacea, Cirripedia, Rhizocephala)." Molecular Phylogenetics and Evolution Vol 41 528 538
15. Glenner H. , Heg J. T. , O'Brien J. J. , Sherman T. D. 2000 "Invasive vermigon stage in the parasitic barnacles Loxothylacus texanus and L. panopaei (Sacculinidae): closing of the rhizocephalan life-cycle." Marine Biology Vol 136 249 257
16. Glenner H. , Ltzen J. , Takahashi T. 2003 "Molecular and morphological evidence for a monophyletic clade of asexually reproducing Rhizocephala: Polyascus, new genus (Cirripedia)." Journal of Crustacean Biology Vol 23 548 557
17. Guillory V. , Perry H. , Steele P. , Wagner T. , Keithly W. , Pellegrin B. , Petterson J. , Floyd T. , Buckson B. , Hartman L. , Holder E. , Moss C. 2001 "The Blue Crab Fishery of the Gulf of Mexico, United States: A Regional Management Plan." Gulf States Marine Fisheries Commission, Ocean Springs, Mississippi
18. Gurney R. H. , Grewe P. M. , Thresher R. E. 2006 "Mitochondrial DNA haplotype variation in the parasitic cirripede Sacculina carcini observed in the cytochrome oxidase gene (COI)." Journal of Crustacean Biology Vol 26 326 330
19. Hanelt B. , Adema C. M. , Mansour M. H. , Loker E. S. 1997 "Detection of Schistosoma mansoni in Biomphalaria using nested PCR." Journal of Parasitology Vol 83 387 394
20. Hochberg R. J. 1992 "Parasitization of Loxothylacus texanus on Callinectes sapidus: aspects of population biology and effects on host morphology." Bulletin of Marine Science Vol 50 117 132
21. Heg J. T. 1987 "Male cypris metamorphosis and a new male larval form, the trichogon, in the parasitic barnacle Sacculina carcini (Crustacea: Cirripedia: Rhizocephala)." Philosophical Transactions of the Royal Society of London. (Series B) Biological Sciences Vol 317 47 63
22. Heg J. T. 1995 "The biology and life cycle of the Rhizocephala (Cirripedia)." Journal of the Marine Biological Association of the United Kingdom Vol 75 517 550
23. Heg J. T. , Glenner H. , Shields J. D. 2005 "Cirripedia Thoracica and Rhizocephala (barnacles), pp. 154-165." In, K. Rhode (ed.), Ecology of Marine Parasites. Blackwell Scientific, Boston, Massachusetts
24. Icely J. D. , Nott J. A. 1992 "Digestion and absorption: digestive system and associated organs, pp. 147-201." In, F. W. Harrison and A. G. Humes (eds.), Microscopic Anatomy of Invertebrates. Vol. 10: Decapod Crustacea. Wiley-Liss, New York
25. Jannotti-Passos L. K. , Magalhes K. G. , Carvalho O. S. , Vidigal T. H D A. 2006 "Multiplex PCR for both identification of Brazilian Biomphalaria species (Gastropoda: PLanorbidae) and diagnosis of infection by Schistosoma mansoni (Trematoda: Schistosomatidae)." Journal of Parasitology Vol 92 401 403
26. John W. 2007 "DNA barcoding in animal species: progress, potential and pitfalls." BioEssays Vol 29 188 197
27. Kaestner A. 1970 "Invertebrate Zoology, Crustacea. Vol. III, (English edition)." Interscience Publishers, New York. 222 pp
28. Kuris A. M. 1974 "Trophic interactions: similarity of parasitic castrators to parasitoids." The Quarterly Review of Biology Vol 49 129 148
29. Kuris A. M. , Lafferty K. D. 1992 "Modelling crustacean fisheries: effects of parasites on management strategies." Canadian Journal of Fisheries and Aquatic Sciences Vol 49 327 336
30. Lawrence M. L. 2001 "An investigation of the invasive vermigon stage of Loxothylacus texanus (Cirripedia: Rhizocephala) that parasitises the blue crab, Callinectes sapidus." Master's thesis. University of South Alabama. 54 pp
31. Levinton J. S. 2001 Marine Biology: Function, Biodiversity, Ecology. Oxford University Press, New York. 50 pp
32. Moritz C. , Cicero C. 2004 "DNA Barcoding: Promise and Pitfalls." PLoS Biology 2, e354
33. Murphy N. E. , Goggin C. L. 2000 "Genetic discrimination of sacculinid parasites (Cirripedia: Rhizocephala): implication for control of introduced green crabs (Carcinus maenas)." Journal of Crustacean Biology Vol 20 153 157
34. Nakayama T. , Watanabe S. , Mitsui K. , Uchida H. , Inouye I. 1996 "The phylogenetic relationship between the Chlamydomonadales and Chlorococcales inferred from 18S rDNA sequence data." Phycological Research Vol 44 47 55
35. National Marine Fisheries Service. 2002 February 26, 2003) (May 18, 2006)
36. Nicol J. A C. 1967 The Biology of Marine Animals. John Wiley and Sons, New York. 595 pp
37. Noble E. R. , Noble G. A. , Shad G. A. , MacInnes A. J. 1989 Parasitology: The Biology of Animal Parasites. Lea and Febiger, Philadelphia, Pennsylvania. 378 pp
38. O'Brien J. J. 1999 "Limb autotomy as an investigatory tool: host molt stage affects the success rate of infective larvae of a rhizocephalan barnacle." American Zoology Vol 39 580 588
39. O'Brien J. J. , Porterfield D. M. , Williams M. D. , Overstreet R. M. 1993 "Effects of salinity and temperature upon host-parasite interactions between the blue crab and the rhizocephalan, Loxothylacus texanus in the Gulf of Mexico." American Zoologist Vol 33 80A
40. O'Brien J. J. , Skinner D. M. 1990 "Overriding of the molt-inducing stimulus of multiple limb autotomy in the mud crab Rhithropanopeus harrisii by parasitization with a rhizocephalan." Journal of Crustacean Biology Vol 10 440 445
41. O'Brien J. J. , van Wyk P. 1985 "Effects of crustacean parasitic castrators (epicaridean isopods and rhizocephalan barnacles) on growth of crustacean hosts, pp. 191-218." In, A. Wenner (ed.), Crustacean Issues: Factors in Adult Growth. Balkema, Rotterdam
42. Overstreet R. M. , Perry H. M. , Adkins G. 1983 "An usually small egg-carrying Callinectes sapidus in the northern Gulf of Mexico, with comments on the barnacle Loxothylacus texanus." Gulf Research Reports Vol 7 293 294
43. Paul D. N H. , Sujeevan R. , Jeremy R. D. 2003 "Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species." Proceedings of the Royal Society B: Biological Sciences Vol 270 S96 S99
44. Prez-Losada M. , Heg J. T. , Kolbasov G. A. , Crandall K. A. 2002 "Reanalysis of the relationships among the Cirripedia and the Ascothoracida and the phylogenetic position of the Facetotecta (Maxillopoda: Thecostraca) using 18S rDNA sequences." Journal of Crustacean Biology Vol 22 661 669
45. Prez-Losada M. , Heg J. T. , Crandall K. A. 2004 "Unraveling the evolutionary radiation of the thoracican barnacles using molecular and morphological evidence: a comparison of several divergence time estimation approaches." Systematic Biology Vol 53 244 264
46. Rasmussen E. 1959 "Behavior of sacculinized shore crabs (Carcinus maenas Pennant)." Nature Vol 183 479 480
47. Ranzani C. 1818 "Osservaxioni su I Balanidi." Opuscoli Scientifici Vol 2 2 63 93
48. Rathbun M. J. 1896 "The genus Callinectes." United States National Museum, Proceedings Vol 18 349 375
49. Rheinsmith E. L. , Hinegardner R. , Bachmann K. 1974 "Nuclear DNA amounts in Crustacea." Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol 48 343 348
50. Ritchie L. E. , Heg J. T. 1981 "The life history of Lernaeodiscus porcellanae (Cirripedia: Rhizocephala) and co-evolution with its porcellanid host." Journal of Crustacean Biology Vol 1 334 347
51. Roberts L. S. , Janovy J. Jr. 2005 Foundations of Parasitology. McGraw-Hill, New York. 550 pp
52. Rognlie M. C. , Dimke K. L. , Knapp S. E. 1994 "Detection of Fasciola hepatica in infected intermediate hosts using RT-PCR." Journal of Parasitology Vol 80 748 755
53. Saiki R. 1985 "Enzymatic amplification of beta-globin sequences and restriction site analysis for diagnosis of sickle cell anemia." Science Vol 230 1350 1354
54. Saiki R. , Getfand D. H. , Stoffel S. , Scharf S. J. , Higuchi R. , Horn G. T. , Mullis K. B. , Erlich H. A. 1988 "Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase." Science Vol 239 487 491
55. Spears T. , Abele L. G. , Applegate M. A. 1994 "Phylogenetic study of cirripedes and selected relatives (Thecostraca) based on 18S rDNA sequence analysis." Journal of Crustacean Biology Vol 14 641 656
56. Takahashi T. , Ltzen J. 1998 "Asexual reproduction as part of the life cycle in Sacculina polygenea (Cirripedia: Rhizocephala: Sacculinidae)." Journal of Crustacean Biology Vol 18 321 331
57. Tsuchida K. , Ltzen J. , Nishida M. 2006 "Sympatric three-species infection by Sacculina parasites (Cirripedia: Rhizocephala: Sacculinidae) of an intertidal grapsoid crab." Journal of Crustacean Biology Vol 26 474 479
58. Williams A. B. 1984 "Shrimps, Lobsters, and Crabs of the Atlantic Coast of the Eastern United States, Maine to Florida." Smithsonian Institution Press, Washington D.C. 550 pp

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