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An effective validated method for HPTLC-fingerprinting of alkaloids and glycosides from multiple plant parts of three Terminalia spp.

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Our study developed a HPTLC fingerprint profile of alkaloids and glycosides obtained from the methanol extracts of four different plant parts of Terminalia arjuna, T. bellerica and T. chebula, trees with cardio-protective values. The multiple qualitative phytochemical analyses of water, acetone, petroleum ether and methanol extracts from all the plant parts of Terminalia spp. detected the presence of alkaloids and glycosides, wherein the methanol extracts exhibited the presence of maximum alkaloids and glycosides. The chromatographic analysis of methanol extracts was carried out on silica gel 60F254HPTLC aluminium sheets with CAMAG Linomat 5 applicator. The plates were developed using ethyl acetate:toluene:formic acid (10:10:1; v/v/v) mobile phase. Alkaloids and glycosides were detected at 254 nm, 366 nm and 540 nm (after derivatization). These developed fingerprints would eventually be of great benefit in identifying or differentiating the alkaloids and glycosides in the form of marker compounds in the three Terminalia spp. mentioned.

Affiliations: 1: Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, Gujarat 384265, India ; 2: Department of Biotechnology, Shri A.N. Patel Post Graduate Institute of Science and Research, Anand, Gujarat 388001, India ; 3: All India Coordinated Research Project on Groundnut, Directorate of Research, Bidhan Chandra Krishi Viswavidyalaya, Kalyani, Nadia, West Bengal 741235, India ; 4: Department of Genetics and Plant Breeding, Faculty of Agriculture, Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal 741252, India


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