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A fast method to prepare microslides of wood in advanced stages of decay

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ABSTRACTBiologically degraded wood in advanced stages of decay has a very soft and brittle structure that causes many problems during sectioning. Embedding wood specimens in different kinds of media ensures preparation of good quality microsections, but the preparation time is very long. The proposed method does not only have a reduced preparation time but also minimizes costs and consumption of chemicals while improving stabilization of the specimen and enhancing the quality of sections. The crux of the method is application of a reinforcing layer of transparent nail polish gel on a dry specimen that has been only stabilized (not embedded) with PEG 1500 medium. The gel is applied on a specimen in two layers just before sectioning. The first layer infiltrates the specimen sufficiently deep to fill the lumens and cell walls and allows preparation of thin sections from decayed wood. The second layer reinforces the section and allows better handling. Subsequently, the reinforcing and embedding layers are removed using pure acetone. This innovative method has so far been successfully tested on specimens that were degraded by the fungus Pleurotus ostreatus (mass loss 55% and 83%) and the fungus Phaeolus schweinitzii (mass loss 45%), taken from Fagus sylvatica and Pinus sylvestris species, a hardwood and softwood respectively with contrasting wide vessels and narrow tracheids.

Affiliations: 1: Department of Wood Science ; 2: Technical University in Zvolen, T.G. Masaryka 24, 960 53 Zvolen, Slovakia ; 3: Institute of Foreign Languages


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1. Barbosa ACF,, Pace MR,, Witovisk L,, Angyalossy V. 2010. "A new method to obtain good anatomical slides of heterogeneous plant parts". IAWA J. Vol 31: 373383.
2. Carlquist S. 1982. "The use of ethylenediamine in softening hard plant structures for paraffin sectioning". Stain Technology Vol 57: 311317. [Crossref]
3. Cousins WJ. 1975. "Optical microscopy of charcoal". J. Microsc. Vol 105: 1518. [Crossref]
4. Čufar K,, Cherubini M,, Gričar J,, Prislan P,, Spina S,, Romagnoli M. 2011. "Xylem and phloem formation in chestnut (Castanea sativa Mill.) during the 2008 growing season". Dendrochronologia Vol 29: 127134. [Crossref]
5. Cutler DF,, Botha T,, Stevenson DW. 2007. Plant anatomy: an applied approach. Wiley-Blackwell, Hoboken.
6. Ferreira BG,, Falcioni R,, Guedes LM,, Avritzer SC,, Antunes WC,, Souza LA,, Isaias RMS. 2017. "Preventing false negatives for histochemical detection of phenolics and lignins in PEG-embedded plant tissues". J. Histochem. Cytochem. Vol 65: 105116. [Crossref]
7. Ferreira BG,, Teixeira CT,, Isaias RMS. 2014. "Efficiency of the polyethylene-glycol (PEG) embedding medium for plant histochemistry". J. Histochem. Cytochem. Vol 62: 577583. [Crossref]
8. Gärtner H,, Schweingruber FH. 2013. Microscopic preparation techniques for plant stem analysis. Verlag Dr. Kessel, Remagen-Oberwinter, Germany.
9. Gjovik LR. 1961. The application of fluorescence microscopy to the study of the permeability of aspen (Populus tremuloides Michx.). MS Thesis, University of Minnesota, Minneapolis.
10. Hamann T,, Smets E,, Lens F. 2011. "A comparison of paraffin and resin-based techniques used in bark anatomy". Taxon Vol 60: 841851.
11. Kukachka BF. 1977. "Sectioning refractory woods for anatomical studies. U.S.D.A. Forest Service", Research Note Vol FPL-0236: 19.
12. Mayer P. 1883. "Einfache Methode zum Aufkleben mikroskopischer Schnitte". Mitt. Zool. Station zu Neapel Vol 2: 521522.
13. Rapp AO,, Behrmann K. 1998. "Preparation of wood for microscopic analysis after decay testing". Holz Roh. Werkst. Vol 56: 277278. [Crossref]
14. Rossi S,, Anfodillo T,, Menardi R. 2006. "Trephor: a new tool for sampling microcores from tree stems". IAWA J. Vol 27: 8997. [Crossref]
15. Rupp P. 1964. "Polyglykol als Einbettungsmedium zum Schneiden botanischer Präparate". Mikrokosmos Vol 53: 123128.
16. Schmitz N,, Verheyden A,, Kairo JG,, Beeckman H,, Koedam N. 2007. "Successive cambia development in Avicennia marina (Forssk.) Vierh. is not climatically driven in the seasonal climate at Gazi Bay, Kenya". Dendrochronologia Vol 25: 8796. [Crossref]
17. Schneider L,, Gärtner H. 2013. "The advantage of using a starch based non-Newtonian fluid to prepare micro sections". Dendrochronologia Vol 31: 175178. [Crossref]
18. Tardif JC,, Conciatori F,. 2015. "Microscopic examination of wood: sample preparation and techniques for light microscopy". In: Yeung EC , et al . (eds.), Plant Microtechniques and Protocols: 373–415. Springer International Publishing, Switzerland.
19. Theiler R. 1973. "Polyäthylenglykol als Hilfsmittel beim Gefrierschneiden". Mikrokosmos Vol 62: 5962.
20. Wilcox WW. 1964. Preparation of decayed wood for microscopical examination. U.S.D.A. Forest Service, Research Note FPL–056: 122.
21. Xing M,, Du Y,, Wang X,, Niu L,, Chen X. 2010. "A simplified paraffin embedding method for small botanical samples". Biotech. Histochem. Vol 85: 241246. [Crossref]

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