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Fungal Colonisation of Douglas Fir and Ponderosa Pine by Poria Carbonica, Coriolus Versicolor, and Chaetomium Globosum; Visualisation With Fluorescent-Coupled Wheat-Germ Agglutinin

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Fluorescent-coupled wheat-germ agglutinin (WGA) was used to examine fungal colonisation of ponderosa pine sapwood and Douglas fir heartwood by two Basidiomycetes and a soft rot fungus. Tracheid lumens were occupied by hyphae within one week of incubation with the three fungi. Three to seven weeks of coloni sation were required before bore holes or other penetrations were evident in the tracheid walls. The WGA technique proved particularly useful at the earliest stages of colonisation when few hyphae were present.


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