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Comparison of the effects of meta-topolin and other cytokinins on chlorophyll and protein contents and peroxidase activity in cucumber cotyledons

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Cytokinins play an important role in controlling many of the processes that contribute to plant senescence. The aim of this study was to compare the effect of different cytokinins, especially the new aromatic cytokinin meta-topolin (mT), with kinetin (K), benzyladenine (BA), and zeatin (Z) on chlorophyll formation in etiolated cucumber cotyledons. Protein contents and peroxidase activities were also investigated. Exogenous application of cytokinins was effective in stimulating chlorophyll biosynthesis. mT was shown to be the most effective cytokinin in stimulating chlorophyll biosynthesis at low concentrations compared to the other cytokinins. mT treatments at 0.02-0.0002 mg/ml increased the amount of chlorophyll more than threefold compared with control cotyledons. BA stimulated the total chlorophyll content at 0.1-10 mg/ml concentrations, but inhibited at lower (0.01, 0.001 mg/ml) concentrations. K increased the chlorophyll amount at 1 mg/ml concentration to twice that of control cotyledons. Z stimulated the chlorophyll level at almost all the concentrations applied. The effects of the most effective concentrations of cytokinins used in this research on the chlorophyll level and on the protein content were examined. A doubling of protein content in 0.02 mg/ml-mT-treated cotyledons was observed, whereas Z, BA, and K increased the total protein to a lesser extent. BA was shown to have the greatest effect on peroxidase activity.

Affiliations: 1: Department of Biology, Faculty of Science, Istanbul University ; 2: Department of Molecular Biology and Genetics, Faculty of Science and Literature, Halic University palavan@superonline.com ; 3: Department of Molecular Biology and Genetics, Faculty of Science and Literature, Halic University

10.1560/216M-053D-12UX-5DBQ
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1. DeBoer, J., Feierabend, J. 1974. Comparison of the effects of cytokinins on enzyme development in different cell compartments of the shoot organs of rye seedlings. Z. Pflanzen-physiol. 71: 261-270.
2. Fukuda, K., Toyama, S. 1982. Electron micrscope studies on the morphogenesis of plastids. XI. Ultrastructural changes of the chloroplasts in tomato leaves treated with ethylene and kinetin. Cytologia 47: 725-736.
3. Palavan-Ünsal, N., Çag, S., Çetin, E. 2002a. Growth responses of excised radish cotyledons to meta-topolin. Can. J. Plant Physiol. 82: 191-194.
4. Legocka, J., Szweykowska, A. 1983. The role of cytokinins in the development and metabolism of barley leaves. VI. The effect on the protein metabolism in various cell compartments during leaf senescence. Acta Physiol. Plant. 5: 11-20.
5. Letham, D.S. 1971. Regulators of cell division in plant tissues. XII. A cytokinin bioassay using excised radish cotyledons. Physiol. Plant. 25: 391-396.
6. Alberte, R.S., Naylor, A.W. 1975. The role of cytokinins in chloroplast lamellar development. Plant Physiol. 55: 1079- 1081.
7. Arnon, D.I. 1949. Copper enzymes in chloroplast polyphenoloxidase in Beta vulgaris. Plant Physiol. 24: 1-15.
8. Legocka, J., Szweykowska, A. 1981. The role of cytokinins in the development and metabolism of barley leaves. III. The effect of RNA metabolism in various cell compartments during senescence. Z. Pflanzenphysiol. 102: 363-374.
9. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248- 254.
10. Chen, C.M., Leisner, S.M. 1985. Cytokinin-modulated gene expression in excised pumpkin cotyledons. Plant Physiol. 73: 76-78.
11. D'Agostino, I.B., Kieber, J.J. 1999. Molecular mechanisms of cytokinin action. Curr. Opinion Mol. Biol. 2: 359-364.
12. Erismann, K.H., Wegner, F. 1967. Der einfluss einer wachstum-shemmenden Kinetin-konzentration auf Chlorophyllgehalt, Photosynthestrate und Starkeproduktion von Lemna minor L. Flora (Jena). Abt. A. 158: 433-442.
13. Galli, M.G. 1984. Synthesis of DNA in excised watermelon cotyledons grown in water and benzyladenine. Planta 160: 193-199.
14. Grover, A., Sinha, S.K. 1985. Senescence of detached leaves in pigeon pea and chick pea: regulation by developing pods. Physiol. Plant. 65: 368-507.
15. Howard, H.F., Witham, F.H. 1983. Invertase activity and kinetin stimulated enlargement of detached radish cotyledons. Plant Physiol. 73: 304-308.
16. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Natura 227: 680- 685.
17. Meidner, H. 1967. The effect of kinetin on stomatal opening and the rate of intake of carbon dioxide in mature primary leaves of barley. J. Exp. Bot. 18: 556-561.
18. Mukherjee, D., Rao, K.V.M. 1993. Alteration patterns of hill activity, peroxidase activity and sugars of pigeon pea during maturation and senescence. Ind. J. Plant Physiol. 36: 13-16.
19. Palavan-Ünsal, N., Çag, S., Çetin, E., Büyüktunçer, D. 2002b. Retardation of senescence by meta-topolin in wheat leaves. J. Cell Mol. Biol. 1: 101-108.
20. Parish, R.W. 1968. Studies on senescing tobacco leaf discs with special reference to peroxidase. I. The effects of cutting and of inhibition of nucleic acid and protein synthesis. Planta 82:1-13.
21. Parthier, B. 1979. The role of phytohormones (cytokinins) in chloroplast development. Biochem. Physiol. Pflanzen 174: 173-214.
22. Purohit, S.S. 1982. Prevention by kinetin of ethylene-induced chlorophyllase activity in senescing detached leaves of Helianthus annuus. Biochem. Physiol. Pflanzen, 177: 625- 627.
23. Fletcher, R.A., Kallidumbil, V., Steele, P. 1982. Stimulation of chlorophyll synthesis in cucumber using cucumber cotyledons. Plant Physiol. 69: 937-939
24. Ford, T.W., Simon, E.W. 1972. Peroxidase and glucose-6-phosphate dehydrogenase levels in cotyledons of Cucumis sativus ( L.). J. Exp. Bot. 23: 423-431.
25. Richmond, A.E., Lang, A. 1957. Effect of kinetin on protein content and survival of detached Xanthium leaves. Science 15: 650-651.
26. Srivastava, S.K., Washi, D.J., Naik, B.L. 1983. Control of senescence by polyamines and guanidines in young and mature barley leaves. Phytochemistry 22: 2151-2154.
27. Strnad, M., Hanus, J., Vanek, T., Kaminek, M. Ballantine, J.A., Fussell, B., Hanke, D.E. 1997. Meta-topolin, a highly active aromatic cytokinin from poplar leaves (Populus canadensis Moench., cv. Robusta). Phytochemistry 45: 213-218.
28. Thimann, K.V. 1980. The senescence of leaves. In: Senescence in plants. CRC Press, Boca Raton, Florida, pp. 85-115.
29. Van Staden, J., Cook, E.L., Nooden, L.D. 1988. Cytokinins and Senescence. In: Senescence and aging in plants. Nooden, L.D. and Leopold, A.C., eds. Academic Press, New York, pp. 281-327.
30. Vassileva, V.S., Dimitrova, A.P. 1984. Influence of zeatin on certain photosynthetic indicators in young pea plants. Biol. Physiol. 37: 1379-1382.
31. Birecka, H., Briber, A., Catalfamo, J.L. 1973. Comparative studies on tobacco pith and sweet potato root isoperoxidases in relation to injury, indolacetic acid and ethylene effects. Plant Physiol. 52: 43-49.
32. Werner, T., Motyka, V. Strnad, M., Schmülling, T. 2001. Regulation of plant growth by cytokinin. Proc. Nat. Acad. Sci. USA 98: 10487-10492.
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2003-05-13
2018-09-21

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