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Isozymic estimation of the biodiversity of Spanish Avena sativa L. landraces stored in a germplasm bank

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Twenty-six accessions from the Spanish national germplasm bank of oats at the Plant Genetic Resources Centre (CRF-INIA) collection of Avena sativa L. were analyzed by isozyme. These accessions were a subset of the core collection and were selected on the basis of their positive agricultural characteristics. The sites where the 26 accessions to the collection were gathered were representative of the relative weightings, both in the core and in the entire collection, of the various agroclimatic regions of Spain. The objectives of the study were: (i) to ascertain the degree of isozymic variability between and within accessions; (ii) to compare the results with the variability recorded in a prior isozyme study with a larger, random, sample of the entire collection in order to validate the criteria used for the selection of the 26 accessions; and (iii) to define groupings of accessions on the basis of their genetic distances, this being a piece of information of value for the utilization of germplasm. Each of the accessions had its own distinctive array of isozyme phenotypes. High overall diversity was detected (phenotypic variability index Pj = 0.576). Almost one-third (29%) of the overall variability was intra-accession variability. There were large differences in intra-accession variability, and accessions were divided into 3 groups on the basis of their internal diversity, estimated from Pj, and the Shannon index, Hj. The isozymic variation in the 26 accessions was similar to that of the entire collection, which indicates that the method used to select the subset did not cause any loss of genetic diversity. The PCA defined 3 clusters of accessions with a few outliers. The grouping showed only a limited link to the geographical origin of the accessions, but had a noteworthy relationship with the agroclimatic conditions at the collection sites.

Affiliations: 1: Escuela Universitaria de Ingeniería Técnica Agrícola INEA fernando.gonzalez@unileon.es ; 2: Departamento de Biología Vegetal, Universidad Politécnica de Madrid ; 3: Escuela Universitaria de Ingeniería Técnica Agrícola INEA ; 4: CRF-INIA

10.1560/B9NP-TG8K-G22G-JU0X
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1. Allard, R.W. 1992. Predictive methods for germplasm identi?-cation. In: Stalker, H.T., Murphy, J.P., eds. Plant breeding in the 1990s. CAB International. Wallingford, U.K., pp. 119–146.
2. Beer, S.C., Goffreda, J., Phillips, T.D., Murphy, J.P., Sorrells, M.E. 1993. Assessment of genetic variation in Avena steri-lis using morphological traits, isozymes, and RFLPs. Crop Science 33 (6): 1386–1393.
3. Brown, A.D.H. 1989. Core collections: a practical approach to genetic resources management. Genome 31: 818–824.
4. Diederichsen, A. 2004. Case studies for the use of infraspeci?cclassi?cationsinmanaginggermplasmcollectionsofculti-vated plants. Acta Horticulturae 634: 127–139.
5. Elías, F., Ruíz Beltrán, L. 1977. Agroclimatología de España. Monografías ICONA. Vol. 7. Ministerio de Agricultura, Pesca y Alimentación, Madrid.
6. Hunter, D.E., II, Murphy, J.P., Phillips, T.D. 1995. Isozyme variation in Avena sterilis L. collected in Turkey. Crop Sci-ence 35 (5): 1477–1482.
7. Goffreda, J., Burnquist, W.B., Beer, S.C., Tanksley, S.D., Sorrells, M.E. 1992. Application of molecular markers to assess genetic relationships among accessions of Wild Oat Avena sterilis. Theoretical and Applied Genetics 85: 146–151.
8. Gómez, C., Sáenz de Miera, L., Pérez de la Vega, M. 1991. Estimation of the isozymatic genetic variability of a Span-ish Avena sativa germplasm collection. Plant Breeding 106 (4): 293–300.
9. Heun, M., Murphy, J.P., Phillips, T.D. 1994. A comparison of RAPD and isozyme analyses for determining the genetic relationships among Avena sterilis L. accessions. Theo-retical and Applied Genetics 87 (6): 689–696.
10. Kahler, A.L., Allard, R.W., Krazkowa, M., Wehrhahn, C.F., Nevo, E. 1980. Associations between isozyme phenotypes and environment in the Slender Wild Oat (Avena barbata) in Israel. Theoretical and Applied Genetics 56: 31–47.
11. Murphy, J.P., Phillips, T.D. 1993. Isozyme variation in culti-vated oats and its progenitor species, Avena sterilis L. Crop Science 33 (6): 1366–1372.
12. Pal, N., Sandhu, J.S., Domier, L.L., Kolb, F.L. 2002. De-velopment and characterization of microsatellite and RFLP-derived PCR markers in oats. Crop Science 42(3): 912–918.
13. Papadakis, J. 1966. Climates of the world and their agricultural potentialities. Editorial Albatros, Buenos Aires. 174 pp.
14. Pérez de la Vega, M., Sáenz de Miera, L.E., Allard, R.W. 1994. Ecogeographical distribution and differential adaptedness of multilocus allelic associations in spanish Avena sativa L. Theoretical and Applied Genetics 88: 56–64.
15. Phillips, T.D., Murphy, J.P. 1993. Distribution and analysis of isozyme polymorphism in North American cultivated oat germplasm. Crop Science 33 (3): 460–469.
16. Plucknett, D.L., Smith, N.J.H., Williams, J.T., Murthi-An-ishetty, N. 1987. Gene Banks and the World’s Food. Princ-eton University Press, Princeton, NJ.
17. Rezai, A., Frey, K.J. 1988. Variation in relation to geo-graphical distribution of wild oats-seed traits. Euphytica 39: 113–118.
18. Rohlf, F.J. 2000. NTSYSpc. Numerical taxonomy and mul-tivariate analysis system. Version 2.1. Exeter Software, New York.
19. Salas, C.A., Murphy, J.P. 1995. Inheritance and linkage of isozyme loci in hexaploid oats. Journal of Heredity 86 (5): 381–385.
20. Phillips, T.D., Murphy, J.P., Goodman, M.M. 1993. Isozyme variation in germplasm accessions of the wild oat Av-ena sterilis L. Theoretical and Applied Genetics 86 (1): 54–64.
21. Torres, E., Iriondo, J.M., Pérez, C. 2003. Genetic structure of an endangered plant, Antirrhinum microphyllum (Scrophu-lariaceae): allozyme and RAPD analysis. American Jour-nal of Botany 90 (1): 85–92.
22. Shannon, C.E., Weaver, W. 1949. The mathematical theory of communication. University of Illinois Press, Urbana, IL.
23. Li, C.D., Rossnagel, B.G., Scoles, G.J. 2000. The devel-opment of oat microsatellite markers and their use in identi-fying relationships among Avena species and oat cultivars. Theoretical and Applied Genetics 101: 1259–1268.
24. Maselli, S., Pérez García, F., Aguinagalde, I. 1999. Evalu-ation of seed storage conditions and genetic diversity of four crucifers endemic to Spain. Annals of Botany 84 (2): 207–212.
25. Matiello, R.R., Neto, J.F.B., Sereno, M.J.C.M., Taderka, I., Pegoraro, D.G. 1998. Genetic variability through isozyme polymorphisms in hexaploid oats. Pesquisa Agropecuaria Brasileira 33 (6): 913–918.
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2005-05-13
2018-06-21

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