Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Application of tissue cultures to overcome the complete sterility of Nicotiana plumbaginifolia Viviani × N. tabacum L. F1 hybrid

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Israel Journal of Plant Sciences

Nicotiana plumbaginifolia Viviani (2n = 20) belongs to the subgenus Petunioides, sec. Alatae. This species was successfully crossed with N. tabacum L. cv. Kroumovgrad 988 (2n = 48) used as pollinator. A small amount of badly developed F0 seeds was formed. Placed on Murashige and Skoog (MS) solid medium with supplements, they produced viable but completely sterile F1 plants with 2n = 34, combining morphological features of both parents. Their stem and leaf morphology was closely related to N. plumbaginifolia, while flower characteristics resembled N. tabacum. The flowers had normal corolla, two-loculed pistils, and stamens with shortened filaments, which provoked weakly expressed longistily. To overcome the F1 hybrid sterility, stem parenchyma was grown in vitro on MS solid medium with different supplements for callus induction, organ formation, and rooting. Regenerants obtained from the 1st to 4th subcultures were fully sterile, while those from the 5th passage were male sterile, but with restored female fertility. Some of the plants from the 7th passage had 32% viable pollen and sufficiently seeded capsules after self-pollination. They were mixoploid with chromosome number 2n = 51-54. Data from comparative electrophoretic analyses of seed storage proteins of N. plumbaginifolia, N. tabacum, and R1 (7th passage) of their F1 hybrid support the presumption that the regenerants carry genes from N. plumbaginifolia. The plants with partially restored fertility are included in our breeding program.

Affiliations: 1: Institute of Genetics, Bulgarian Academy of Sciences


Full text loading...


Data & Media loading...

1. Hammersley-Straw, D.R.H., Thorpe, T.A. 1987. Modulation of shoot formation in tobacco callus by tissue transfer between permissive and inhibitory media. In Vitro Cell Dev. Biol. 23, 12: 867-872.
2. Nikova, V., Pundeva, R., Petkova, A. 1999. Nicotiana tabacum L. as a source of cytoplasmic male sterility in interspecific cross with N. alata Link & Otto. Euphytica 107: 9-12.
3. Burk, L.G. 1960. Male-sterile flower anomalies in interspecific tobacco hybrids. J. Hered. 51: 27-31.
4. Davis, B.J. 1964. Disc electrophoresis. II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci. 121: 404-427.
5. Goodspeed, H.T. 1954. The genus Nicotiana. Chron. Bot. 16: 403-405.
6. Huetteman, C.A., Preece, J.E. 1993. TDZ: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult. 33: 105-119.
7. Nikova, V.M., Zagorska, N.A., Pundeva, R.S. 1991. Development of four tobacco cytoplasmic male sterile sources using in vitro techniques. Plant Cell Tissue Organ Cult. 27: 289-295.
8. Murashige, T., Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
9. Nikova, V.M., Zagorska, N.A. 1984. Variability of regenerants obtained in tissue culture of Nicotiana velutina Wheeler ´ N. tabacum L. Proc. 3rd Natl. Conf. Cytogenet. Plovdiv, Bulgaria, Vol. 1, pp. 191-194.
10. Nikova, V.M., Zagorska, N.A. 1990. Overcoming hybrid incompatibility between Nicotiana africana Merxm. and N. tabacum L. and development of cytoplasmically male sterile tobacco forms. Plant Cell Tissue Org. Cult. 23: 71- 75.
11. Kostoff, D. 1943. Cytogenetics of the genus Nicotiana. Sofia State Printing House, pp. 18-22.
12. Reynolds, J.F. 1987. Chemical regulation in tissue culture: an overview. XII Intern. Hort. Congr. 83rd ASHS Ann. Meet. Davis, Calif., 15 Aug. 1986. HortScience 22, 6 (special issue): 1192-1193.
13. Skirvin, R.M., Mc Pheeters, K.D., Norton, M. 1994. Sources and frequency of somaclonal variation. Hort. Sci. 29: 1232-1237.
14. Sorrentino, C., Del Piano, L., Abet, M., Tonini, A., Barbato, L. 1998. Somaclonal variation in regenerated plants from Nicotiana rustica (R) callus after five culturing years. Il Tobacco 6: 45-53.
15. Nikova, V., Palakarcheva, M., Pundeva, R., Krusteva, D. 1998. Somaclonal variation in cultured in vitro tobacco plants from the hybrid Nicotiana gossey domin. ´ N. tabacum L. Isr. J. Plant Sci. 46: 35-40.
16. D'Amato, F. 1978. Chromosome number variation in cultured cells and regenerated plants. In: Thorpe, T.A., ed. Frontiers of plant tissue culture. Univ. Calgary Press, pp. 287-295.
17. D'Amato, F. 1991. Nuclear changes in cultured plant cells. Caryologia 44: 217-224.
18. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. Nature 227: 680-685.
19. Murthy, B.N.S., Murch, S.J., Saxena, P.K. 1998. Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev. Biol. Plant 34, 4: 267-275.
20. Vladova, R., Petkolicheva, K., Pandeva, R., Nikova, V. 1996/ 1997. Electrophoretic study of seed storage proteins in the genera Capsicum and Nicotiana. Genet. Breed. 28, 1-2: 7- 12.
21. Zagorska, N. 1976. Utilization of tissue cultures for genetic investigations and in the breeding of crop plants. Agric. Sci. 14, 3: 13-27 (in Bulgarian).
22. Zagorska, N., Shamina, Z.B., Butenko, R.G. 1971. Morphogenetic regularities in tissue cultures in diploid and poliploid tobacco. Compt. Rend. AS USSR 196, 5: 219- 222.

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Israel Journal of Plant Sciences — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation