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Application of tissue cultures to overcome the complete sterility of Nicotiana plumbaginifolia Viviani × N. tabacum L. F1 hybrid

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Nicotiana plumbaginifolia Viviani (2n = 20) belongs to the subgenus Petunioides, sec. Alatae. This species was successfully crossed with N. tabacum L. cv. Kroumovgrad 988 (2n = 48) used as pollinator. A small amount of badly developed F0 seeds was formed. Placed on Murashige and Skoog (MS) solid medium with supplements, they produced viable but completely sterile F1 plants with 2n = 34, combining morphological features of both parents. Their stem and leaf morphology was closely related to N. plumbaginifolia, while flower characteristics resembled N. tabacum. The flowers had normal corolla, two-loculed pistils, and stamens with shortened filaments, which provoked weakly expressed longistily. To overcome the F1 hybrid sterility, stem parenchyma was grown in vitro on MS solid medium with different supplements for callus induction, organ formation, and rooting. Regenerants obtained from the 1st to 4th subcultures were fully sterile, while those from the 5th passage were male sterile, but with restored female fertility. Some of the plants from the 7th passage had 32% viable pollen and sufficiently seeded capsules after self-pollination. They were mixoploid with chromosome number 2n = 51-54. Data from comparative electrophoretic analyses of seed storage proteins of N. plumbaginifolia, N. tabacum, and R1 (7th passage) of their F1 hybrid support the presumption that the regenerants carry genes from N. plumbaginifolia. The plants with partially restored fertility are included in our breeding program.

Affiliations: 1: Institute of Genetics, Bulgarian Academy of Sciences

10.1560/HRXE-EB7A-1UU3-82HD
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2004-05-13
2018-06-21

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