Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Light absorption and fluorescence, and photoacclimation features in the marine macroalga Porphyra leucosticta (Rhodophyta)

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Israel Journal of Plant Sciences

Absorption and fluorescence spectra, photosynthetic rates, pigment concentrations, and cell size were investigated during photoacclimation of Porphyra leucosticta, a red marine macroalga that thrives during winter in the eastern Mediterranean. Optical properties were measured on intact thalli using image analysis or from crude extracts after disrupting algal tissue. Absorption spectra from crude extracts showed major peaks at 485, 620, and 664 nm for chlorophyll a (chl a) and 500, 564 and 615 nm for phycoerythrin (PE) and phycocyanin (PC). Absorption peaks from intact thalli were less conspicuous than those from crude extracts, possibly due to PE contributing to the bulk absorption of light by chl a in intact cells. Fluorescence emission of intact thalli was about threefold higher than that of crude extracts at 420 and 560 nm. Image analysis revealed differences in maximal fluorescence emissions as related to cell size, cell age, and thallus functional areas, with female cells having nearly a twofold higher emission than vegetative cells or asexual spores. Changes in the fluorescence emissions observed during acclimation to irradiance could reflect the extent of energy transfer from PE and PC to chl a, variable photosynthetic efficiency, or optical properties of the samples. This study suggests that image analysis can provide insights on seaweed photoacclimation that seem to be lost when disrupting their tissues to crude extracts. For example, the high PE to chl a ratios observed with image analysis in fresh tissues may confer a unique photoprotective role of PE when P. leucosticta becomes exposed to high irradiances, even during winter time.

Affiliations: 1: National Institute of Oceanography, Israel Oceanographic and Limnological Research ; 2: The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University ; 3: National Institute of Oceanography, Israel Oceanographic and Limnological Research


Full text loading...


Data & Media loading...

1. Algarra, P., de la Viña, G., Niell, F. X. 1991. Effects of light quality and irradiance level on short-term pigment response of the red alga Corallina elongata.Mar. Ecol. Prog. Ser. 74: 27-32.
2. Beer, S., Eshel, A. 1985. Determining phycoerythrin and phycocyanin concentrations in aqueous crude extracts of red algae. Aust. J. Mar. Freshw. Res. 36: 785-792.
3. Ben-Zion, M., Dubinsky, Z. 1988. An on-line system for measuring photosynthetic characteristics via an oxygen-electrode. J. Plankton Res. 10: 555-558.
4. Berner, T., Wyman, K., Dubinsky, Z., Falkowski, P. G. 1989. Photoadaptation and the "package" effect in Dunaliella tertiolecta (Chlorophyceae). J. Phycol. 25: 70-78.
5. Cordi, B., Depledge, M. H., Price, D. N., Salter, L. F., Donkin, M. E. 1997. Evaluation of chlorophyll fluorescence, in vivo spectrophotometric pigment absorption and ion leakage as biomarkers of UV-B exposure in marine macroalgae. Mar. Biol. 130: 41-49.
6. Cordi, B., Hyde, P., Donkin, M. E., Price, D. N., Depledge, M. H. 1999. Evaluation of in vivo thallus absorption and chlorophyll fluorescence as biomarkers of UV-B exposure and effects in marine macroalgae from different tidal levels. Mar. Environ. Res. 48: 193-212.
7. Duarte, P., Ferreira, J. G. 1995. Seasonal adaptation and short-term metabolic responses of Gelidium sesquipedale to varying light and temperature. Mar. Ecol. Prog. Ser. 121: 289-300.
8. Dubinsky, Z. 1992. The optical and functional cross-sections of phytoplankton photosynthesis. In: Falkowsky, P. G., Woodhead, A. D., eds., Primary productivity and biogeochemical cycles in the sea. Plenum, New York, pp. 31-45.
9. Enriquez, S., Agusti, S., Duarte, C. M. 1994. Light absorption by marine macrophytes. Oecologia. 98: 121-129.
10. Figueroa, F. L., Aguilera, G., Jimenez, C. 1995. Growth pigments synthesis and nitrogen assimilation in the red alga Porphyra sp. (Bangiales, Rodophyta) under blue and red light. Sci. Mar. 59: 9-20.
11. Figueroa, F. L., Ruiz, R., Saez, E., Lucena, J., Niell, F. X. 1997. Spectral light attenuation and phytoplankton distribution during a daily cycle in the reservoir of La Concepcion, Southern Spain. Arch. Hydrobiol. 140: 71-90
12. Friedlander, M. 1992. Gracilaria conferta and its epiphytes: the effect of culture conditions on growth. Bot. Mar. 35: 423-428.
13. Grzymski, J., Johnsen, G., Sakshaug, E., 1997. The significance of intracellular selfshading on the biooptical properties of brown, red, and green macroalgae. J. Phycol. 33: 408-414.
14. Hannach, G. 1989. Spectral light absorption by intact blades of Porphyra abbottae (Rodophyta): effect of environmental factors in culture. J. Phycol. 25: 522-529.
15. Henley, W. 1993. Measurements and interpretation of photosynthetic light-response curves in algae in the context of photoinhibition and diel changes. J. Phycol. 29: 729-739.
16. Israel, A., Katz, S., Dubinsky, Z., Merrill, J. E., Friedlander, M. 1999. Photosynthetic inorganic carbon utilization and growth of Porphyra linearis (Rhodophyta). J. Appl. Phycol. 11: 447-453.
17. Israel, A., Levy, I., Friedlander, M. 2006. Experimental tank cultivation of Porphyra in Israel. J. Appl. Phycol. (in press).
18. Katz, S., Dubinsky, Z., Rothmann, C., Malik, Z., Friedlander, M. 1997. Single-cell pigmentation of Porphyra linearis analyzed by Fourier transform multi-pixel spectroscopy and image analysis. J. Phycol. 33: 425-432.
19. Katz, S., Kizner, Z., Dubinsky, Z., Friedlander, M. 2000. Responses of Porphyra linearis (Rhodophyta) to environmental factors under controlled culture conditions. J. Appl. Phycol. 12: 535-542.
20. Levitt, G. J., Bolton, J. J. 1991. Seasonal patterns of photosynthesis and physiological parameters and the effects of emersion in littoral seaweeds. Bot. Mar. 34: 403-410.
21. Lipkin, Y., Beer, S., Eshel, A. 1993. The ability of Porphyra linearis (Rhodophyta) to tolerate prolonged periods of dessication. Bot. Mar. 36: 517-523.
22. Lobban, C. S., Harrison, P. J. 1994. Seaweed ecology and physiology. Cambridge University Press, New York. pp. 366.
23. López-Figueroa, F., Niell, F. X. 1990. Effects of light quality on chlorophyll and biliprotein accumulation in seaweeds. Mar. Biol. 104: 321-327.
24. Lüning, K., Dring, M. J. 1985. Action spectra and spectral quantum yield of photosynthesis in marine macroalgae with thin and thick thalli. Mar. Biol. 87: 119-129.
25. Malik, Z., Dishi, M. 1995. Optical methods for tumor treatment and detection: mechanisms and techniques in photodynamic therapy IV. SPIE. 2392: 152-158.
26. Markager, S. 1993. Light absorption and quantum yield for growth in five species of marine macroalgae. J. Phycol. 29: 54-63.
27. Mercado, J. M., Jimenez, C., Niell, F. X., Figueroa, F. L. 1996. Comparison of methods for measuring light absorption by algae and their application to the estimation of the package effect. Sci. Mar. 60: 39-45.
28. Merrill, J. E., Mimuro, M., Aruga, Y., Fujita, Y. 1983. Light harvesting of photosynthesis in four strains of red algae Porphyra yezoensis having different phycobilin content. Plant Cell Physiol. 24: 261-266.
29. Moran, R. 1982. Formulae for determination of chlorophyllous pigments extracted with N, N-dimethylformamide. Plant Physiol. 69: 1376-1381.
30. Owens, T. G., Gallagher, J. C., Alberte, R. S. 1987. Photosynthetic light-harvesting function of violaxanthin in Nannochloropsis spp. (Eustigmatophyceae). J. Phycol. 23: 79-85.
31. Pedersen, A., Kraemer, G., Yarish, C. 2004. The effect of temperature and nutrient concentrations on nitrate and phosphate uptake in different species of Porphyra from Long Island Sound (USA). J. Exp. Mar. Biol. Ecol. 312: 235-252.
32. Prezelin, B. B. 1981. Light reactions in photosynthesis. In: Platt, T., ed., Physiological basis of phytoplankton ecology. Can. Bull. Fish. Aquat. Sci. 210, Ottawa, pp. 1-43.
33. Provasoli, L. 1968. Media and prospects for cultivation of marine algae. In: Watanabe, A., Hattari, A., eds., Cultures and collections of algae. Proc. US-Japan Conf. Hakonte. Jpn. Soc. Plant Physiol., pp. 63-75.
34. Ramus, S. J. 1978. Seaweed anatomy and photosynthetic performance: the ecological significance of light guides heterogeneous absorption and multiple scatter. J. Phycol. 14: 352-362.
35. Rudiger, W., Figueroa, L. F. 1992. Photoreceptors in algae. Photochem. Photobiol. 55: 949-954.
36. Schanz, F., Senn, P., Dubinsky, Z. 1997. Light absorption by phytoplankton and the vertical light attenuation: Ecological and physiological significance. Annu. Rev. Oceanogr. Mar. Biol. 35: 71-95.
37. Smith, C. M., Alberte, R. S. 1994. Characterization of in vivo absorption features of chlorophyte, phaeophyte and rhodophyte algal species. Mar. Biol. 118: 511-521.
38. Stambler, N., Dubinsky, Z. 2005. Corals as light collectors: an integrating sphere approach. Coral Reefs. 24: 1-9.
39. Stirk, W. A., Aken, M. E., Staden, G. V. 1995. Effect of irradiance on photosynthesis in a filamentous red alga (Ceramiaceae, Rhodophyta). S. Afr. Tydskr. Plantk. 61: 153-157.
40. Talarico, L., Maranzana, G. 2000. Light and adaptive responses in red macroalgae: an overview. J. Photochem. Photobiol. 56: 1-11.
41. Varela-Alvarez, E., Stengel, D. B. Guiry, M. D. 2004. The use of image processing in assessing conchocelis growth and conchospore production in Porphyra linearis.Phycologia 43: 282-287.
42. Zavodnik, N. 1987. Seasonal variations in the rate of photosynthetic activity and chemical composition of the littoral seaweeds Ulva rigida and Porphyra leucosticta from the North Adriatic. Bot. Mar. 30: 71-82.

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Israel Journal of Plant Sciences — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation