Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Effects of soil water content on Mentha spicata L. and Origanim dictamnus L.

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Israel Journal of Plant Sciences

The effects of four levels of soil water content (100%, 80%, 60%, 40% SWC) on M. spicata and O. dictamnus were studied. Drought stress (40% SWC) resulted in a great reduction of the plant height and biomass in both plants. Leaves decreased in size and exhibited signs of chlorosis with local reddish patches. Leaf anatomical observations principally comprised an accumulation of anthocyanins in the vacuoles of the mesophyll cells and a diminishing of the intercellular space volume. Leaf blade thickness did not undergo any alteration in M. spicata, whereas in O. dictamnus it became greatly reduced. The decrease of SWC from 100% to 40% reflected an increase in the number of leaf peltate hairs producing the essential oil, an increase in the leaf essential oil yield, an increase in the density of mesophyll cells, and a decrease in the number of stomata in both plants. Furthermore, drought stress had a negative effect on chlorophyll a + b, chlorophyll fluorescence, photosynthetic rate, transpiration rate, and stomatal conductance. GC-MS analysis of the essential oils derived from control M. spicata and O. dictamnus plants (100% SWC) and from drought-stressed plants (40% SWC) disclosed differences in their qualitative and quantitative composition.

Affiliations: 1: Department of Botany, School of Biology, Aristotle University ; 2: Department of Plant Production, Technological Educational Institute ; 3: Department of Pharmacognosy-Pharmacology, School of Pharmacy, Aristotle University


Full text loading...


Data & Media loading...

1. Adams, R. 2001. Identification of essential oil components by gas chromatography/mass spectroscopy. Carol Stream. Allured Publ., Illinois.
2. Amelunxen, F., Wahlig, T., Arbeiter, H. 1969. �ber den Nachweis des �therischen �ls in isolierten Dr�senhaaren und Dr�senschuppen von Mentha piperita L. Z. Pflanzen-physiol. 61: 68-72.
3. Basker, D., Putievsky, E. 1978. Seasonal variation in the yields of herb and essential oil in some Labiatae species. J. Hortic. Sci. 53: 179-183.
4. Bisio, A., Ciavallo, G., Romussi, G., Fontanta, N., Mascolo, N., Capasso, R., Biscardi, D. 1998. Chemical composition of essential oils from some Salvia species. Phytoth. Res. 12: S117-S120.
5. Bosabalidis, A.M. 2002. Structural features of Origanum sp. In: Kintzios, S.E., ed. Oregano. The genera Origanum and Lippia. Taylor and Francis, London, pp. 11-64.
6. Brun, N., Colson, M., Perrin, A., Voirin, B. 1991. Chemical and morphological studies of the effects of ageing on monoterpene composition in Mentha x piperita leaves. Can. J. Bot. 69: 2271-2278.
7. Cakmak, I. 1994. Activity of ascorbate-dependent H2O2-scavenging enzymes and leaf chlorosis are enhanced in magnesium-deficient and potassium-deficient leaves, but not in phosphate-deficient leaves. J. Exp. Bot. 45: 1259-1266.
8. Chartzoulakis, K., Patakas, A., Bosabalidis, A. 1999. Comparative study on gas exchange, water relations and leaf anatomy of two olive cultivars grown under well-irrigated and drought conditions. Z. Naturforsch. 54: 688-692.
9. Chartzoulakis, K., Patakas, A., Kofidis, G., Bosabalidis, A., Nastou, A. 2002. Water stress affects leaf anatomy, gas exchange, water relations and growth of two avocado cultivars. Sci. Horticult. 95: 39-50.
10. Davies, N.N. 1990. Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methylsilicone and Carbowax 20M phases. J. Chromat. 503: 1-24.
11. El-Keltawi, N.E., Croteau, R. 1987. Salinity depression of growth and essential oil formation in spearmint and majoram and its reversal by foliar applied cytokinin. Phytochemistry 26: 1333-1334.
12. Fischer, G., Hecht-Buchholz, Ch. 1985. The influence of boron deficiency on glandular scale development and structure in Mentha piperita. Planta Med. 51: 371-377.
13. Gavalas, N., Bosabalidis, A.M., Kokkini, S. 1998. Comparative study of leaf anatomy and essential oils of the hybrid Mentha x villoso-nervata and its parental species M. longifolia and M. spicata. Isr. J. Plant Sci. 46: 27-33.
14. Gerschenzon, J., Maffei, M., Croteau, R. 1989. Biochemical and histochemical localization of monoterpene biosynthesis in the glandular trichomes of spearmint (Mentha spicata). Plant Physiol. 89: 1351-1357.
15. Gortzi, O., Lalas, S., Chinou, I., Tsaknis, J. 2007. Evaluation of the antimicrobial and antioxidant activities of Origanum dictamnus extracts before and after encapsulation in liposomes. Molecules 12: 932-945.
16. Grammatikopoulos, G., Karousou, R., Kokkini, S., Manetas, Y. 1998. Differential effects of enhanced UV-B radiation on reproductive effort in two chemotypes of Mentha spicata under field conditions. Aust. J. Plant Physiol. 25: 345-351.
17. Granger, R., Passet, J., Verdier, R. 1964. Le γ-terpinene pre-curseur du p-cymene dans Thymus vulgaris. Compt. Rend. Hebd. Seances Acad. Sci. 258: 5539-5541.
18. Hernandez, I, Alegre, L., Munne-Bosch, S. 2006. Enhanced oxidation of flavan-3-ols and proanthocyanidin accumulation in water-stressed tea plants. Phytochemistry 67: 1120-1126.
19. Ilias, I., Ouzounidou, G., Giannakoula, A., Papadopoulos, P. 2007. Effects of gibberellic acid and prohexadione-calcium on growth, chlorophyll fluorescence and quality of okra plant. Biol. Plant. 51: 575-578.
20. Karousou, R., Vokou, D., Kokkini, S. 1998. Variation of Salvia fruticosa essential oils on the island of Crete (Greece). Bot. Acta 111: 250-254.
21. Katsiotis, S., Oikonomou, G.N. 1986. Vergleichende Untersuchung verschiedener wildwachsender und in Kreta angebauter Muster von Origanum dictamnus. L. Sci. Pharmac. 54: 49-52.
22. Kofidis, G., Bosabalidis, A.M., Chartzoulakis, K. 2004. Leaf anatomical alterations induced by drought stress in two avocado cultivars. J. Biol. Res. 1: 115-120.
23. Kokkini, S. 1983. Taxonomic studies on the genus Mentha L. in Greece. Doctorate thesis. School Biol. Univ. Thessaloniki.
24. Kokkini, S, Vokou, D. 1989. Mentha spicata (Lamiaceae) chemotypes growing wild in Greece. Econ. Bot. 43: 192-202.
25. Krol, M., Gray, G.R., Hurry, V.M. 1995. Low temperature stress and photoperiod affect an increased tolerance to photoinhibition in Pinus banksiana seedlings. Can. J. Bot. 73: 1119-1127.
26. Lincoln, D.E., Murray, M.J., Lawrence, B.M. 1986. Chemical composition and genetic basis for the isopinocamphone chemotype of Mentha citrata hybrids. Phytochemistry 25: 1857-1863.
27. Massada, Y. 1976. Analysis of essential oils by gas chromatography and spectrometry. Wiley & Sons, New York.
28. McCaskill, D. Gerschenzon, J., Croteau, R. 1992. Morphology and monoterpene biosynthetic capabilities of secretory cell clusters isolated from glandular trichomes of peppermint (Mentha piperita L.). Planta 187: 445-454.
29. Nagata, T., Todoriki, S., Masamizu, T., Suda, I., Furuta, S., Du, Z., Kikuchi, S. 2003. Levels of active oxygen species are controlled by ascorbic acid and anthocyanin in Arabidopsis. J. Agric. Food Chem. 51: 2992-2999.
30. Ouzounidou, G., Moustakas, M., Symeonidis, L., Karataglis, S. 2006. Response of wheat seedlings to Ni stress: effects of supplemental calcium. Arch. Environ. Contam. Toxicol. 50: 346-352.
31. Panou-Filotheou, H., Bosabalidis, A.M., Karataglis, S. 2001. Effects of copper toxicity on leaves of oregano (Origanum vulgare subsp. hirtum). Ann. Bot. 88: 207-214.
32. Papadakis, I.E., Dimassi, K.N., Bosabalidis, A.M., Therios, I.N., Patakas, A., Giannakoula, A. 2004. Effects of B excess on some physiological and anatomical parameters of "Navelina" orange plants grafted on two rootstocks. Environm. Exp. Bot. 51: 247-257.
33. Patakas, A., Noitsakis, B. 2001. Leaf age effects on solute accumulation in water-stressed grapevines. J. Plant Physiol. 158: 63-69.
34. Patakas, A., Nikolaou, N., Zioziou, E., Radoglou, K., Noitsakis, B. 2002. The role of organic solute and ion accumulation in osmotic adjustment in drought-stressed grapevines. Plant Sci. 163: 361-367.
35. Perrin, A., Colson, M. 1991. Timing of the harvest date for Mentha x piperita based on observations of the floral development. J. Ess. Oil Res. 3: 17-25.
36. Pickett-Heaps, J.D. 1969. Preprophase microtubule bands in some abnormal mitotic cells of wheat. J. Cell Sci. 4: 397-420.
37. Ramanjulu, S., Veeranjaneyulu, K., Sudhakar, C. 1993. Physiological changes induced by NaCl in mulberry var. Mysore local. Ind. J. Plant Physiol. 36: 273-275.
38. Sabatini, D.D., Bensch, K., Barrnett, B.J. 1963. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17: 19-58.
39. Schaden, G., Hesse, C. 1976. �ber das �therische �l des kre-tischen Diptams. Origanum dictamnus L. Monatshefte f. Chim. 107: 929-931.
40. Sivropoulou, A., Papanikolaou, E., Nikolaou, C., Kokkini, S., Lanaras, Th., Arsenakis, M. 1996. Antimicrobial and cytotoxic activities of Origanum essential oils. J. Agric. Food Chem. 44: 1202-1205.
41. Skrubis, B. 1979. Origanum dictamnus L., a Greek native plant. J. Ethnopharm. 1: 411-415.
42. Spurr, A.R. 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26: 31-43.
43. Sylvertsen, J.P., Lloyd, J., McConchie, C., Kriedemann, P.E., Farquhar, G.D. 1995. On the relationship between leaf anatomy and CO2 diffusion through the mesophyll of hypostomatous leaves. Plant, Cell Environm. 18: 149-157.
44. Van den Dool, H., Kratz, P.D. 1963. A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. J. Chromat. 11:463-471.
45. Vigo, C., Therios, I.N., Patakas, A., Karatassou, M., Nastou, A. 2002. Changes in photosynthetic parameters and nutrient distribution of olive plants (Olea europaea L.) cultivar "chondrolia Chalkidikis" under NaCl, Na2SO4 and KCl salinities. Agrochimica 46: 35-41.
46. Wintermans, J.B., Mots, A. 1965. Spectrophotometric characteristics of chlorophyll a and b and their pheophytins in ethanol. Biochem. Biophys. Acta 109: 448-453.

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Israel Journal of Plant Sciences — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation