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Extraction of Genomic DNA from roots and leaves of Hylocereus undatus

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Since most DNA extraction protocols require young leaves to extract high quality DNA, seed germination is a useful in providing starting material for DNA extraction. In the case of Hylocereus undatus, since the fruit contains thousand of seeds, seed germination can be used to produce material in abundance for DNA extraction by harvesting the seeds from the mature fruit. In this study, sodium bisulfite, sodium sarkosyl, and sorbitol were added to the DNA isolation buffer. Sodium bisulphite prevents oxidation of phenolic compounds, whereas sodium sarkosyl precipitates high levels of polysaccharides. The use of this modified DNA extraction method resulted in high-yield DNA. The result was reproducible and the DNA can be stored in TE buffer at -20 °C for months without degradation, can be digested using BamH1, and can then be used for construction of a library.

Affiliations: 1: Institute of Biological Sciences, Faculty of Science, University of Malaya


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