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Development of a RAPD-based SCAR marker for sex identification in Momordica dioica Roxb

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Momordica dioica Roxb., commonly known as spine gourd, is a perennial, rhizomatous, distinctly dioecious climber belonging to the family Cucurbitaceae. There is an urgent need for sex identification at pre-flowering stage to support selection and breeding of this plant because of its sex-specific economic values. Since there are no morphological markers to identify sex, an easy, rapid and reliable molecular method for sex identification at the pre-flowering stage in M. dioica is reported in this paper. Both Random amplification of polymorphic DNA (RAPD) and sequence-characterized amplified region (SCAR) markers were used to search for specific markers linked to the sex locus. A total of 50 random decamer primers were used for screening of specific RAPD markers in male and female populations. Only one primer, OPA-15, amplified genomic DNA in different patterns in the male versus female genotypes. This sex-specific band OPA-151500 was cloned and sequenced. Based on the RAPD sequence, a pair of SCAR primers MSSM-01F and MSSM-01R was developed. These SCAR primers amplified a single 1501 bp DNA band only in male populations. This band was subsequently named SDSM (Sequence Discriminating Sex of Momordica dioica). These SCAR primers should be useful in identifying markers associated with sex determination in M. dioica.

Affiliations: 1: Department of Applied Genetics, Karnatak Science College


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