Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

The use of laser scanning confocal microscopy in the study of wound healing phenomena in plants

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Israel Journal of Plant Sciences

Wound tissues in plants frequently exhibit high amounts of cells with thick, lignified, or suberized walls and a contorted course, so that they are difficult to be observed in the microscope. Since laser scanning confocal microscopy (LSCM) offers the principal capacity to overcome some of these imaging problems via optical sectioning processes, experiments were performed with different wound tissues to examine its possible applications. While young, delicate tissues could be observed without further preparation even in the living state, for older, denser tissues it proved to be necessary to infiltrate the specimens with immersion oil or a resin with a high refractive index to allow three-dimensional reconstructions of cell relations in depth. Parenchymatic tissue thus prepared allowed the greatest depth of light penetration, while lignified tissue absorbed the light more readily, and suberized tissue did not become transparent at all. In several cases, image quality could be substantially improved and clearer spatial relations of the various cell types were achieved.

Affiliations: 1: Institut für Forstbotanik und Baumphysiologie, Albert-Ludwigs-Universität


Full text loading...


Data & Media loading...

1. Biggs, A.R., Stobbs, L.W. 1986. Fine structure of the suberized cell walls in the boundary zone and necrophylactic periderm in wounded peach bark. Can. J. Bot. 64: 1606-1610.
2. Fink, S. 1992. Transparent wood—a new approach in the functional study of wood structure. Holzforschung 46: 403-408.
3. Fink, S. 1999. Pathological and regenerative plant anatomy. Encyclopedia of plant anatomy, Vol. XIV, 6. Gebrueder Borntraeger, Berlin, Stuttgart, 1095 pp.
4. Hawkins, S., Boudet, A. 1996. Wound-induced lignin and suberin deposition in a woody angiosperm (Eucalyptus gunnii Hook.): histochemistry of early changes in young plants. Protoplasma 191: 96-104.
5. Haynes, J.G., Czymmek, K.J., Carlson, C.A., Veereshlingam, H., Dickstein, R., Sherrier, D.J. 2004. Rapid analysis of legume root nodule development using confocal microscopy. New Phytol. 163: 661-668.
6. Hepler, P.K., Gunning, B.E.S. 1998. Confocal fluorescence microscopy of plant cells. Protoplasma 201: 121-157.
7. Hibbs, A.R. 2004. Confocal microscopy for biologists. Springer, New York, 474 pp.
8. Jaccard, P. 1910. Wundholzbildung im Mark von Picea excelsa.Ber. Dt. Bot. Ges. 28: 62-71.
9. Kagayama, M., Sasano, Y., Hirata, M., Mizoguchi, I., Takahashi, I. 1996. An improved mounting method for observation of thick specimens using confocal microscopy. Biotech. Histochem. 71: 231-233.
10. Kitin, P., Funada, R., Sano, Y., Ohtani, J. 2000. Analysis by confocal microscopy of the structure of cambium in the hardwood Kalopanax pictus.Ann. Bot. 86: 1109-1117.
11. Kitin, P., Sano, Y., Funada, R. 2001. Analysis of cambium and differentiating vessel elements in Kalopanax pictus using resin cast replicas. IAWA J. 22: 15-28.
12. Kitin, P., Sano, Y., Funada, R. 2003. Three-dimensional imaging and analysis of differentiating secondary xylem by confocal microscopy. IAWA J. 24: 211-222.
13. Lemon, G.D., Posluszny, U. 1998. A new approach to the study of apical meristem development using laser scanning confocal microscopy. Can. J. Bot. 76: 899-904.
14. Lowerts, G, Wheeler, E.A., Kellison, R.C. 1986. Characteristics of wound-associated wood of yellow poplar (Liriodendron tulipifera L.). Wood Fiber Sci. 18: 537-552.
15. Mäule, C. 1896. Der Faserverlauf im Wundholz. Bibl. Bot. 33: 1-34.
16. Moell, M.K., Donaldson, L.A. 2001. Comparison of segmentation methods for digital image analysis of confocal microscope images to measure tracheid cell dimensions. IAWA J. 22: 267-288.
17. Ogata, Y., Fujita, M. 2005. New anatomical method of grain angles measurement using confocal microscopy and image cross-correlation. Trees 19: 73-80.
18. Paddock, S.W., ed. 1999. Confocal microscopy: methods and protocols. Humana Press, Totowa, New Jersey.
19. Pawley, J., ed. 2006. Handbook of biological confocal microscopy. 3rd ed. Springer, New York, 988 pp.
20. Prior, D.A.M., Oparka, K.J., Roberts, I.M. 1999. En bloc optical sectioning of resin-embedded specimens using a confocal laser scanning microscope. J. Microsc. 193: 20-27.
21. Smith, D.E. 1980. Abnormal wood formation following fall and spring injuries in black walnut. Wood Sci. 12: 243-251.
22. Wymer, C.L., Beven, A.F., Boudonck, K., Lloyd, C.W. 1999. Confocal microscopy of plant cells. In: Paddock, S.W., ed. Confocal microscopy: methods and protocols. Humana Press, Totowa, New Jersey, pp. 103-130.

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Israel Journal of Plant Sciences — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation