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Histological investigation of the leaf and leaf-originating calli of Lavandula vera L.

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Structures or cells with secreting activity were localized in the leaves of Lavandula vera. Light and electron microscopy, both TEM and SEM, were applied for the investigation of the secreting sites. A series of histochemical reactions was applied and a spectrum of metabolites produced was identified. Terpenes, flavonoids, steroids, alkaloids, and various types of phenolics were present. Leaf tissue was cultured and callus was produced. Callus cell masses were also investigated for their structure and secreting activity. Callus cells were found to be synthetically active and to produce—as leaf cells do—compounds of potential interest.

Affiliations: 1: Department of Botany, Faculty of Biology, University of Athens


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1. Arnold, S. von, Eriksson, T. 1981. In vitro studies of adventitious shoot formation in Pinus conforta. Can. J. Bot. 59: 870-874.
2. Ascensao, L., Mota, L., De Castro, M. 1999. Glandular trichomes on the leaves and flowers of Plectranthus ornatus: morphology, distribution and cytochemistry. Ann. Bot. 84: 437-447.
3. Balandrin, M.J., Klocke, J.A. 1988. Medicinal, aromatic and industrial materials from plants. In: Bajaj, Y.P.J., ed. Biotechnology in agriculture and forestry. Medicinal and aromatic plants. Vol. 4. Springer-Verlag, Berlin, pp. 1-36.
4. Banthorpe, D.V., Bilyard, H.J., Watson, D.G. 1985. Pigment formation by callus of Lavandula angustifolia. Phytochemistry 24: 2677-2680.
5. Bottega, S., Corsi, G. 2000. Structure, secretion and possible functions of calyx glandular hairs of Rosmarinus officinalis L. (Labiatae). Bot. J. Linn. Soc. 134: 325-335.
6. Bourgaud, F., Gravot, A., Milesi, S., Gontier, E. 2001. Production of secondary metabolites: a historical perspective. Plant Sci. 161: 839-851.
7. Bronner, R. 1975. Simultaneous demonstration of lipid and starch in plant tissues. Stain Technol. 50: 1-4.
8. Cappelletti, E.M., Caniato, R., Appendino, G. 1986. Localization of cytotoxic hydroperoxyeudesmanolides in Artemisia umbelliformis. Biochem. Syst. Ecol. 14: 183-190.
9. Christodoulakis, N.S. 1989. An anatomical study of seasonal dimorphism in the Leaves of Phlomis fruticosa.Ann. Bot. 63: 389-394.
10. Christodoulakis, N.S. 1992. Structural diversity and adaptations in some mediterranean Evergreen sclerophyllous species. Environ. Exp. Bot. 32: 295-305.
11. Christodoulakis, N.S., Fasseas, C. 1990. Air pollution effects on the leaf structure of Laurus nobilis, an injury resistant species. Bull. Environ. Contam. Toxicol. 44: 276-281.
12. Christodoulakis, N.S., Bazos, J. 1990. Leaf anatomy of three seasonally dimorphic subshrubs. Acta Oecol. 11: 291-296.
13. Christodoulakis, N.S., Fasseas, C. 1991. Seasonal dimorphism of Phlomis fruticosa under controlled environmental conditions. Acta Oecol. 12: 323-330.
14. Christodoulakis, N.S., Tsimbani, H., Fasseas, C. 1990. Leaf structural peculiarities in Sarcopoterium spinosum: a seasonally dimorphic subshrub. Ann. Bot. 65: 291-300.
15. Corsi, G., Bottega, S. 1999. Glandular hair of Salvia officinalis: new data on morphology, localization and histochemistry in relation to function. Ann. Bot. 84: 657-664.
16. Dioscorides,~70 ce. De materia medica. Militos Publications, Athens [2001].
17. Dronne, S., Jullien, F., Caissard, J.-C., Faure, O. 1999. A simple and efficient method for in vitro shoot regeneration from leaves of lavandin (Lavandula X intermedia Emmeric ex Loiseleur). Plant Cell Rep. 18: 429-433.
18. Farnsworth, N.R. 1985. The role of medicinal plants in drug development. In:. Kroogsgard-Larsen, P., Brogger-Christenses, S., eds. Natural products and drug development. Munsgaard, Copenhagen, pp. 17-30
19. Faure, G. 1914. Manuale di micrografia vegetale. Roma: Instituto Nazionale Medico Farmacologico Editore.
20. Fisher, D.B. 1968. Protein staining of ribboned epon sections for light microscopy. Histochemie 16: 92-96.
21. Fowler, M.W., Scragg, A.H. 1988. Natural products from higher plants and plant cell culture. In: Pais, M.S.S., Mavituna, F., Novais, J.M., eds. Plant cell biotechnology. NATO ASI Series, Vol. 18. Springer—Verlag, Berlin, pp. 165-177.
22. Furr, M., Mahlberg, P.G. 1981. Histochemical analyses of laticifers and glandular trichomes in Cannabis sativa. J. Nat. Prod. 44: 153-159.
23. Gardner, R.O. 1975. Vanillin—hydrochloric acid as a histochemical test for tannin. Stain Technol. 50: 315-317.
24. Gersbach, P.V., Wyllie, S.G., Sarafis, V. 2001. A new histochemical method for localization of the site of monoterpene phenol accumulation in plant secretory structures. Ann. Bot. 88: 521-525.
25. Guerin, H.P., Delaveau, P.G., Paris, R.R. 1971. Localisations histochimiques. II: Procedes simples de localisation de pigments flavoniques. Application a quelques Phanerogrames. Bull. Soc. Bot. France 118: 29-36.
26. Hardman, R., Sofowora, E.A. 1972. Antimony trichloride as a test reagent for steroids, especially diosgenin and yamogenin, in plant tissues. Stain Technol. 47: 205-222.
27. Johansen, D.A. 1940. Plant microtechnique. McGraw-Hill, New York.
28. Kovatcheva, E., Pavlov, A., Koleva, I., Ilieva, M., Mihneva, M. 1996. Rosmarinic acid from Lavandula vera MM cell culture. Phytochemistry 43: 1243-1244.
29. Kovatcheva, E., Koleva, I., Ilieva, M., Pavlov, A., Mincheva, M., Konushlieva, M. 2001. Antioxidant activity of extracts from Lavandula vera MM cell culture. Food Chem. 72: 295-300.
30. Ledbetter, M., Porter, K. 1963. A "microtubule" in plant cell fine structure. J. Cell Biol. 19: 239-250.
31. Lison, L. 1960. Histochemie et cytochemie animales. Principes et methodes. Vols. 1, 2. Gauthier-Villars, Paris.
32. Mace, M.E., Howell, C.R. 1974. Histochemistry and identification of condensed tannin precursors in root of cotton seedlings. Can. J. Bot. 52: 2423-2426.
33. Mowry, R.W. 1956. Alcian blue techniques for the histochemical study of acidic carbohydrates. J. Histochem. Cytochem. 4: 407-411.
34. O'Brien, T.P., McCully, M.E. 1981. The study of plant structure principals and selected methods. Termacarphy Pty. Ltd., Melbourne, Australia.
35. Payne, G.F., Bringi, V., Prince, C., Shuler, M.L. 1991. Plant cell and tissue culture in liquid systems. Hanser Publications, Munich, pp. 1-10.
36. Phillipson, J.D. 1990. Plants as a source of valuable products. In: Charlwood, B.V., Rhodes, M.J.C., eds. Secondary products from plant tissue culture. Clarendon Press, Oxford, pp. 1-21.
37. Ramachandra, R.S., Ravishankar, G.A. 2002. Plant cell cultures: chemical factories of secondary metabolites. Biotechnol. Adv. 20: 101-153.
38. Reeve, R.M. 1951. Histochemical tests for polyphenoles in plant tissues. Stain Technol. 26: 91-96.
39. Reynolds, E. 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17: 19-58.
40. Sabatini, D., Bensch, K., Barrnett, B. 1963. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol. 17: 208-212.
41. Sacchetti, G., Romagnoli, C., Nicoletti, M., Di Fabio, A., Bruni, A., Poli, F. 1999. Glandular trichomes of Calceolaria adscendens Lidl. (Scrophulariaceae): histochemistry, development and ultrastructure. Short communication. Ann. Bot. 83: 87-92.
42. Tutin, T.G., Heywood, V.H., Burges, N.A., Moore, D.M., Valentine, D.H., Walters, S.M., Webb, D.A., eds., 1999. Flora Europaea. Cambridge University Press, Cambridge.

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