Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Direct shoot organogenesis from different explants of chrysanthemum, marigold, and tuberose

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Israel Journal of Plant Sciences

Regeneration protocols for direct differentiation of shoots have been developed from different explants of some of the commercially important cultivars of Chrysanthemum morifolium Ramat., F1 hybrid of Tagetes erecta L., and Polianthes tuberosa L. Leaf explants of chrysanthemum differentiated shoot buds in the presence of 6-benzylaminopurine (BA) and indole-3-acetic acid (IAA), while the flower head and receptacle differentiated in the presence of BA and a-naphthaleneacetic acid (NAA). Triiodobenzoic acid played a very crucial role for induction of shoots in leaf explant of F1 hybrid of marigold. Thidiazurone along with NAA induced shoots in the ray florets of marigold. In tuberose, nodular structures were formed in the presence of higher concentrations of BA + gibberellic acid and AgNO3, but the further development of shoot buds from those nodules was possible only when these concentrations were lowered in the presence of abscisic acid and IAA. The plantlets regenerated from different explants were transferred to pots and then to the field, where they flowered true-to-mother plant.

Affiliations: 1: Floriculture Section, National Botanical Research Institute


Full text loading...


Data & Media loading...

1. Mandal, A.K.A., Chakrabarty, D., Datta, S.K. 2000a. In vitro development of novel flower colour through management of induced chimera. Euphytica 114: 9-12
2. Mandal, A.K.A., Chakrabarty, D., Datta, S.K. 2000b. Use of in vitro techniques in mutation breeding of chrysanthemum. Plant Cell Tissue Org. Cult. 60: 33-38.
3. Misra, P., Datta, S.K. 2000. In vitro maintenance of F hybrid. Curr. Sci. 78: 383-384.
4. Murashige, T., Skoog, F. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue culture. Physiol. Plant. 15: 473-491.
5. Sanyal, M., Dutta Gupta, S., Jana, M.K., Kundu, S.C. 1998. Shoot organogenesis and plant regeneration from leaf callus cultures of tuberose (Polianthes tuberosa L.). Plant Tiss. Cult. Biotechnol. 4: 81-86.
6. Bush, S.R., Earle, E.D., Langhans, R.W. 1976. Plantlets from petal segments, petal epidermis and shoot tips of the periclinal chimera, Chrysanthemum morifolium 'Indianapolis'. Am. J. Bot. 63: 729-737.
7. Chakrabarty, D., Mandal, A.K.A., Datta, S.K. 1999. Management of chimera through direct shoot regeneration from florets of chrysanthemum (Chrysanthemum morifolium Ramat.). J. Hortic. Sci. Biotech. 74: 293-296.
8. Chakrabarty, D., Mandal, A.K.A., Datta, S.K. 2000. Retrieval of new coloured chrysanthemum through organogenesis from sectorial chimeras. Curr. Sci. 78: 1060-1061.
9. Datta, S.K., Chakrabarty, D., Mandal, A.K.A. 2001. Gamma ray induced genetic variation and their manipulation through tissue culture. Plant Breeding 120: 91-92.
10. Malaure, R.S., Barclay, G., Power, J.B., Davey, M.R. 1991. The production of novel plants from florets of Chrysanthemum morifolium using tissue culture 1. Shoot regeneration from ray florets and somaclonal variation exhibited by the regenerated plants. J. Plant Physiol. 139: 8-13.

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Israel Journal of Plant Sciences — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation