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A Preliminary Study on the Problems in the Preparation of Artemia Parthenogenetica Chromosomes for Scanning Electron Microscopy

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AbstractThe chromosomes of Crustacea, especially decapods, are usually numerous, small in size, and punctiform. These characteristics make it difficult for researchers to further investigate the chromosomes by light microscopy. As an alternative, the scanning electron microscopy (SEM) is recommended because it has higher resolution and is able to provide valuable three-dimensional data and X-ray microanalysis. The aim of this study is to obtain some fundamental knowledge for the preparation of the chromosomes of crustaceans for SEM observations. Since crustacean chromosomes are so small, impurities such as mucus and cytoplasm easily obscure the chromosome surface making it impossible for researchers to observe their fine structure. We used Artemia parthenogenetica as a model and confirmed impurities originating from the nauplii paste and the cytoplasm of the cells, and demonstrate that the nauplii paste can be removed by rinsing and the cytoplasm can be digested by trypsin.

Affiliations: 1: (T-HL, SS) Graduate School of Fisheries Science, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan


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1. Abatzopoulos J., Beardmore J. A., Clegg J. S., Sorgeloos P. 2002 "Artemia: Basic and Applied Biology." Kluwer Academic Publishers
2. Abatzopoulos J., Kastritsis C. D., Triantaphyllidis C. D. 1986 "A study of karyotypes and heterochromatic associations in Artemia, with special reference to two N. Greek populations." Genetica Vol 71 3 10
3. Allen T. D., Jack E. M., Harrison C. J., Claugher D. 1988 "The three dimensional structure of human metaphase chromosomes determined by scanning electron microscopy, pp. 51-72." In, K. W. Adolph (ed.), Chromosomes and chromatin. Vol. 2, CRC Press, Boca Raton
4. Barigozzi C., Valsasnini P., Ginelli E., Badaracco G., Plevani P., Baratelli L. 1987 "Further data on repetitive DNA and speciation in Artemia, pp. 103-105." In, P. Sorgeloos, D. A. Bengtson, W. Decleir, and E. Jaspers (eds.), Artemia Research and applications. Vol. 1, Universa Press, Wetteren, Belgium
5. Barigozzi C. 1994 "The chromosomes of the Artemia living in the Salt Lake of Larnaca, Cyprus." Rendiconti Fisica Accademia dei Lincei Vol 5 363 365
6. Browne R. A., Sorgeloos P., Trotman C. N A. 1991 "Artemia Biology." CRC Press, Boca Raton
7. Evans E. P., Breckson G., Ford C. E. 1964 "An air-drying method for meiotic preparations from mammalian testes." Cytogenetics Vol 3 289 294
8. Harrison C. J., Jack E. M., Allen T. D., Harris R. 1985 "Light and scanning electron microscopy of the same metaphase chromosomes." Journal Cell Science Vol 77 143 153
9. Harrison C. J., Jack E. M., Allen T. D. 1987 "Light and scanning electron microscopy of the same metaphase chromosomes, pp. 189-249." In, M. A. Hayat (eds.), Correlative microscopy in biology: Instrumentation and methods Academic Press, Orlando
10. Iino A. 1975 "Human somatic chromosomes observed by scanning electron microscope." Cytobios Vol 14 39 48
11. Kingsley-Smith B. V. 1970 "The application of scanning and transmission electron microscopy to a study of whole chromosomes." Micron Vol 2 39 57
12. Lecher P., Defaye D., Noel P. 1995 "Chromosomes and nuclear DNA of Crustacea." Invertebrate Reproduction and Development Vol 27 85 114
13. Lee T-H. 1990 "Chopping machine for chromosome preparation." IDEN (Tokyo) Vol 44 75 76
14. Lee T-H., Naitoh N., Yamazaki F. 2004 "Chromosome studies on the mitten crabs Eriocheir japonica and E. sinensis." Fisheries Science Vol 70 211 214
15. Murofushi M., Deguchi Y. 1983 "A method for obtaining metaphase chromosomes from large shrimp-like crustaceans." Report of Mishima Research Institute of Science and Living, Nihon University Vol 6 31 34
16. Sanchez-Sweatman O. H., Harven E. P., Dube I. D. 1993 "Human chromosomes: Evaluation of processing techniques for scanning electron microscopy." Scanning Microscopy Vol 7 97 106
17. Seabright M. 1971 "A rapid banding technique for human chromosomes." Lancet Vol 11 971 972
18. Seed T. M., Kaspar L. V., Domann F., Niiro G. K., LeBuis D. A. 1988 "Developmental and radiobiologic characteristics of canine multinucleated, osteoclast-like cells generated in vitro from canine bone marrow." Scanning Microscopy Vol 2 1599 1611
19. Sharma A. K., Sharma A. 1994 "Chromosome Techniques: A Manual." Harwood Academic Publishers
20. Sorgeloos P., Bengston D. A., Decleir W., Jaspers E. 1987 "Artemia research and its applications." Vol. 1, Universa Press, Wetteren, Belgium
21. Sumner A. T. 1996 "Problems in preparation of chromosomes for scanning electron microscopy to reveal morphology and to permit immunocytochemistry of sensitive antigens." Scanning Microscopy (Supplement) Vol 10 165 176
22. Sumner A. T., Evans H. J., Buckland R. 1971 "New techniques for distinguishing between human chromosomes." Nature (London) New Biology Vol 232 31 32
23. Yamazaki F., Onozato H., Arai K. 1981 "The chopping method for obtaining permanent chromosome preparation from embryos of teleost fishes." Bulletin Japanese Society of Scientific Fisheries Vol 47 963

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