Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Sensory Setae on the Major Chelae of Male Crayfish, Orconectes Rusticus (Decapoda: Astacidae) – Impact of Reproductive State on Function and Distribution

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

AbstractThe major chelae of reproductive male crayfish contain both smooth and plumose setae and are used for the perception of female odors. A comparative and morphological analysis of setae found on the major chelae of reproductive (form I) and non-reproductive (form II) male crayfish, Orconectes rusticus was performed in order to elucidate the distribution and function of smooth and plumose setae. In particular, the distribution of setae between reproductive forms was quantified as well as putative sensory functions, based on morphological characteristics, for both smooth and plumose setae. To accomplish these goals, scanning electron microscopy, a porosity assay, anterograde labeling, and acetylated tubulin (AT) immunocytochemistry were used. We found that form I crayfish have significantly more pockets of sensory setae and individual smooth setae on their major chelae when compared to form II males. Scanning electron microscopy revealed a terminal apical pore-like structure in smooth setae that was absent in plumose setae. Congruent with this finding, smooth setae absorbed crystal violet stain and DiI which labeled putative neural fibers. Conversely, plumose setae did not show any crystal violet or DiI staining. Furthermore, smooth setae contain fiber-like processes that are AT-immunoreactive and are located below the chitinous exoskeleton of the chelae. These fibers extend from the base of the smooth setae to the pore-like structure located at the distal tip. AT-immunoreactive fibers were not present in plumose setae. These results imply that smooth setae on the major chelae are putative chemoreceptors and plumose setae may serve a mechanosensory function. Coupled with previous behavioral studies, these results suggest that a dimorphism in the major chelae, found between male reproductive forms, enhances sensory perception during reproductive behaviors.

Affiliations: 1: a(RB, PM) J.P. Scott Center for Neuroscience, Mind & Behavior, Laboratory for Sensory Ecology, Department of Biological Sciences, Bowling Green State University, Bowling Green, Ohio 43403, U.S.A. (RB,; PM, correspondence: ; 2: b(KM, SC, XR, BZ) Department of Biological Sciences, University of Windsor, Windsor, Ontario, Canada N9B 3P4 (KM,; SC,; XR,; BZ,


Full text loading...


Data & Media loading...

1. Abello P., Warman C. G., Reid D. G., Naylor E. 1994 "Chela loss in the shore crab Carcinus maenas (Crustacea: Brachyura) and its effect on mating success." Marine Biology Vol 121 247 252
2. Altner H., Hatt H., Altner I. 1983 "Structural and functional properties of the mechanoreceptors and chemoreceptors in the anterior esophageal sensilla of the crayfish, Astacus astacus." Cell and Tissue Research Vol 228 357 374
3. Ameyaw-Akumfi C., Hazlett B. A. 1975 "Sex recognition in the crayfish Procambarus clarkii." Science Vol 190 1225 1226
4. Ball E. E., Cowan A. N. 1977 "Ultrastructure of the antennal sensilla of Acetes (Crustacea, Decapoda, Natantia, Sergestidae)." Transactions of the Royal Society of London B Vol 277 429 456
5. Belanger R. M., Moore P. A. 2006 "The use of the major chelae by reproductivemale crayfish (Orconectes rusticus) for discrimination of female odours." Behaviour Vol 143 713 731
6. Belanger R. M., Smith C. M., Corkum L. D., Zielinski B. S. 2003 "Morphology and histochemistry of the peripheral olfactory organ in the round goby, Neogobius melanostomus (Teleostei: Gobiidae)." Journal of Morphology Vol 257 62 71
7. Bauer U., Hatt H. 1980 "Demonstration of 3 different types of chemosensitive units in the crayfish claw using a computerized evaluation." Neuroscience Letters Vol 17 209 214
8. Bender M., Gnatzy W., Tautz J. 1984 "The antennal feathered hairs in the crayfish: a non-innervated stimulus transmitting system." Journal of Comparative Physiology A Vol 154 45 47
9. Bundy M. H., Paffenhöfer G-A. 1993 "Innervation of copepod antennules investigated using laser scanning confocal microscopy." Marine Ecology Progress Series Vol 102 1 14
10. Cate H. S., Derby C. D. 2002a "Ultrastructure and physiology of the hooded sensillum, a bimodal chemo-mechanosensillum of lobsters." Journal of Comparative Neurology Vol 442 293 307
11. Cate H. S., Derby C. D. 2002b "Hooded sensilla homologues: Structural variations of a widely distributed bimodal chemomechanosensillum." Journal of Comparative Neurology Vol 444 345 357
12. Corotto F. S., Bonenberger D. M., Bounkeo J. M., Dukas C. C. 1999 "Antennule ablation, sex discrimination, and mating behavior in the crayfish Procambarus clarkii." Journal of Crustacean Biology Vol 19 708 712
13. Crouau Y. 1981 "Cytology of various antennal setae in the troglobitic Mysidacea (Crustacea)." Zoomorphology Vol 98 121 134
14. Crocker D. W., Barr D. W. 1968 "Handbook of the Crayfish of Ontario." University of Toronto Press, 158 pp
15. Derby C. D. 1989 "Physiology of sensory neurons in morphologically identified cuticular sensilla of crustaceans, pp. 27-47." In, B. Felgenhauer, L. Watling, and A. B. Thistle (eds.), Functional morphology of feeding and grooming in Crustacea. A.A. Balkema Publishers, Rotterdam
16. Derby C. D. 1982 "Structure and function of cuticular sensilla of the lobster Homarus americanus." Journal of Crustacean Biology Vol 2 1 21
17. Drach P., Jacques F. 1980 "Système sétifère des crustacés décapods." Comptes rendus de l'Académie des sciences Vol 290 1435 1438
18. Dunham D. W., Oh J. W. 1992 "Chemical sex discrimination in the crayfish Procambarus clarkii: role of antennules." Journal of Chemical Ecology Vol 18 2363 2371
19. Felgenhauer B. E., Abele L. G. 1983 "Ultrastructure and functional morphology of feeding and associated appendages in the tropical fresh-water shrimp Atya innocuous (Herbst) with notes on its ecology." Journal of Crustacean Biology Vol 3 336 363
20. Garm A., Derby C. D., Høeg J. T. 2004 "Mechanosensory neurons with bend- and osmo-sensitivity in mouthpart setae from the spiny lobster Panulirus argus." Biological Bulletin Vol 207 195 208
21. Garm A. 2004 "Revising the definition of the crustacean seta and setal classification systems based on examinations of the mouthpart setae of seven species of decapods." Zoological Journal of the Linnean Society Vol 142 233 252
22. Gill C. W. 1986 "Suspected mechano- and chemosensory structures of Temora longicornis (Copepoda: Calanoida)." Marine Biology Vol 93 449 457
23. Girard C. 1852 "A revision of the North American Astici, with observations on their habits and geographic distribution." Proceedings of the Academy of Natural Sciences of Philadelphia Vol 6 87 91
24. Gleeson R. A. 1980 "Pheromone communication in the reproductive behaviour of the blue crab, Callinectes sapidus." Marine Behaviour and Physiology Vol 7 119 134
25. Gleeson R. A. 1982 "Morphological and behavioral identification of the sensory structures mediating pheromone reception in the blue crab, Callinectes sapidus." Biological Bulletin Vol 163 162 171
26. Hatt H., Bauer U. 1980 "Single unit analysis of mechanosensitive and chemosensitive neurons in the crayfish claw." Neuroscience Letters Vol 17 203 207
27. Hatt H. 1986 "Responses of a bimodal neuron (chemo- and vibration-sensitive) on the walking legs of crayfish." Journal of Comparative Physiology A Vol 159 611 617
28. Hindley J. P R., Alexander C. G. 1978 "Structure and function of the chelae periopods of the banana prawn Penaesus merguiensis." Marine Biology Vol 48 153 160
29. Jacques F. 1989 "The setal system of crustaceans: Types of setae, groupings, and functional morphology, pp. 1-13." In, B. Felgenhauer, L. Watling, and A. B. Thistle (eds.), Functional morphology of feeding and grooming in Crustacea. A.A Balkema Publishers, Rotterdam
30. Juanes F., Smith L. D. 1995 "The ecological consequences of limb damage and loss in decapod crustaceans: A review and prospectus." Journal of Experimental Marine Biology and Ecology Vol 193 197 223
31. Kamio M., Araki M., Nagayama T., Matsunaga S., Fusetani H. 2005 "Behavioral and electrophysiological experiments suggest that the antennular outer flagellum is the site of pheromone reception in the male hermit crab Telmessus cheiragonus." Biological Bulletin Vol 208 12 19
32. Keller T. A., Hazlett B. A. 1996 "Mechanical use of crayfish chelae." Marine and Freshwater Behaviour and Physiology Vol 28 149 162
33. Lavalli K. L., Factor J. R. 1995 "The feeding appendages, pp. 349-393." In, J. R. Factor (ed.), The Biology of the Lobster, Homarus americanus. Academic Press, NY
34. Laverack M. S. 1988 "The nervous system of Crustacea with special reference to the organization of the sensory system, pp. 323-351." In, M. A. Ali (ed.), Nervous Systems in Invertebrates. Platinum Press, NY
35. Lereboullet A. 1858 "Description des deux nouvelles especies d'écrevisses de nos rivieres." Memoires de la Societe des Sciences Naturelles Vol 5 1 11
36. McIver S. B. 1975 "Structure of cuticular mechanoreceptors of arthropods." Annual Review of Entomology Vol 20 381 397
37. Mauchline J., Aizawa Y., Ishimaru T., Nishids S., Marumo R. 1977 "Integumental sensilla of pelagic Decapod crustaceans." Marine Biology Vol 43 149 155
38. Nation J. L. 1983 "A new method using hexamethyldisilazane for preparation of soft insect tissues for scanning electron microscopy." Stain Technology Vol 58 347 351
39. Obermeier M., Schmitz B. 2004 "The modality of the dominance signal in snapping shrimp (Alpheus heterochaelis) and the corresponding setal types on the antennules." Marine and Freshwater Behaviour and Physiology Vol 37 109 126
40. Schmidt M., Derby C. D. 2005 "Non-olfactory chemoreceptors in asymmetric setae activate antennular grooming behavior in the Caribbean spiny lobster Panulirus argus." Journal of Experimental Biology Vol 208 233 248
41. Schmidt M., Gnatzy W. 1984 "Are the funnel-canal organs the ‘campaniform sensilla’ of the shore crab, Carcinus maenas (Decapoda, Crustacea)? II." Ultrastructure. Cell and Tissue Research Vol 237 81 93
42. Sekkelsten G. I. 1988 "Effect of handicap on mating success in male shore crabs Carcinus maenas." Oikos Vol 51 131 134
43. Shelton R. G J., Laverack M. S. 1968 "Observations on a redescribed crustacean cuticular sense organ." Comparative Biochemistry and Physiology Vol 25 1049 1059
44. Shelton R. G J., Laverack M. S. 1970 "Receptor structure and function in the lobster Homarus gammarus (L)." Journal of Experimental Marine Biology and Ecology Vol 4 201 210
45. Siddiqui S. S., Aamodt E., Rastinejad F., Culotti J. 1989 "Anti-tubulin monoclonal antibodies that bind to specific neurons in Caenorhabditis elegans." Journal of Neuroscience Vol 9 2963 2972
46. Slifer E. H. 1960 "A rapid and sensitive method for identifying permeable areas in the body wall of insects." Entomological News Vol 71 179 182
47. Snedden W. A. 1990 "Determinants of mating success in the temperate crayfish Orconectes rusticus: Chela size and sperm competition." Behaviour Vol 115 100 113
48. Solon M. H., Cobb J. S. 1980 "The external morphology and distribution of cuticular hair organs on the claws of the American lobster, Homarus americanus (Milne-Edwards)." Journal of Experimental Marine Biology and Ecology Vol 48 205 215
49. Stein R. A. 1976 "Sexual dimorphism in crayfish chelae: functional significance linked to reproductive activities." Canadian Journal of Zoology Vol 54 220 227
50. Stein R. A., Murphy M. L., Magnuson J. J. 1977 "External morphological changes associated with sexual maturity in the crayfish (Orconectes propinquus)." American Midland Naturalist Vol 97 495 502
51. Thomas W. J. 1970 "The setae of Austropotamobius pallipes (Crustacea: Astacidae)." Journal of Zoology, London Vol 160 91 142
52. Tierney A. J., Thompson C. S., Dunham D. W. 1984 "Site of pheromone reception in the crayfish Orconectes propinquus (Decapoda, Cambaridae)." Journal of Crustacean Biology Vol 4 554 559
53. Tierney A. J., Thompson C. S., Dunham D. W. 1986 "Fine structure of aesthetasc chemoreceptors in the crayfish Orconectes propinquus." Canadian Journal of Zoology Vol 64 132 152
54. Vogt G., Tolley L. 2004 "Brood care in freshwater crayfish and relationship with offspring's sensory deficiencies." Journal of Morphology Vol 262 566 582
55. Watling L. 1989 "A classification system for crustacean setae based on the homology concept, pp. 15-26." In, B. Felgenhauer, L. Watling, and A. B. Thistle (eds.), Functional morphology of feeding and grooming in Crustacea. A.A Balkema Publisher, Rotterdam
56. Weisbaum D., Lavalli K. L. 2004 "Morphology and distribution of antennular setae of scyllarid lobsters (Scyllarides aequinoctialis, S. latus, and S. nodifer) with comments on their possible function." Invertebrate Biology Vol 123 324 342
57. Weissberg M. J., Derby C. D. 1995 "Regulation of sex-specific feeding behavior in fiddler crabs: physiological properties of chemoreceptor neurons in claw and legs of males and females." Journal of Comparative Physiology A Vol 176 513 526

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Journal of Crustacean Biology — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation