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Effects of Salinity Stress on Survival, Metabolism, Limb Regeneration, and Ecdysis in Uca Pugnax

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Abstract Estuarine intertidal zones are characterized by variable salinity regimes due to seasonal and daily fluctuations. Salt-marsh inhabitants, such as the mud fiddler crab Uca pugnax, are physiologically adapted to this habitat. Crustacean growth and development are energy demanding and sensitive hormonal processes which are susceptible to natural stressors. This study investigated physiological and metabolic changes in the molt cycle of U. pugnax in response to different salinity levels by assessing the survival, respiration, and post-molt tissue protein and carbohydrate content. Crabs were subjected to a limb regeneration and ecdysis challenge under four different salinity regimes. For the study, a limb was removed and its regenerative growth was photographed every two days. After ecdysis, crabs were dissected, and the tissues collected were analyzed for their protein and carbohydrate contents. Crabs held at 60 and 75 ppt seawater showed 100% mortality. Crabs at 60 ppt did not undergo basal limb growth. During limb regeneration, abnormal limb growth occurred solely in males. Post-molt 40 ppt crabs showed elevated respiration rates compared to 10 ppt crabs. Females and 25 ppt crabs had significantly more free carbohydrates in epithelial tissue than males and crabs at 10 and 40 ppt. As salinity decreased, epithelial protein content in females decreased while males experienced no change. Salinity did not affect the amount of acid, water or base-soluble exoskeleton protein. These physiological responses indicate that U. pugnax males and individuals exposed to extreme salinities are more likely to be impacted by salinity stress during limb regeneration and ecdysis.

Affiliations: 1: Biology Department, West Virginia University, Morgantown, West Virginia 26506


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1. Adam P. 1993 Saltmarsh ecology Cambridge University Press Cambridge, UK
2. Allen B. J. , Levinton J. S. 2007 "Costs of bearing a sexually selected ornamental weapon in a fiddler crab." Functional Ecology Vol 21 154 161
3. Bosc L. A. G. 1802 Manuel de l'histoire naturelle des crustacés, contenant leur description et leurs moeus; avec Fig.s dessinèes d'apés nature. Volume 1 Deterville Paris
4. Bradford M. 1976 "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding." Analytical Biochemistry Vol 72 248 254
5. Brodie R. J. , Behum M. E. , Monroe E. , Glenn N. , Staton J. L. 2005 "Recruitment to adult habitats following marine planktonic development in the fiddler crabs, Uca pugilator, U. pugnax, and U. minax." Marine Biology Vol 147 105 111
6. Cauchie H. M. , Jaspar-Versali M. F. , Hoffman L. , Thome J. P. 1999 "Analysis of the seasonal variation in biochemical composition of Daphnia magna Straus, (Crustacea: Branchiopoda: Anomopoda) from an aerated wastewater stabilization pond." Annales de Limnologie Vol 35 223 231
7. Chang E. S. 1995 "Physiological and biochemical changes during the molt cycle in decapod crustaceans: an overview." Journal of Experimental Biology and Ecology Vol 193 1 14
8. Chang E. S. , Chang S. A. , Mulder E. P. 2001 "Hormones in the lives of crustaceans: an overview." American Zoologist Vol 41 1090 1097[1090:HITLOC]2.0.CO;2
9. Clare A. S. , Costlow J. D. , Bedair H. M. 1992 "Assessment of crab limb regeneration as an assay for developmental toxicity." Canadian Journal of Fisheries and Aquatic Science Vol 49 1268 1273
10. Gagné F. , Blaise C. , Pellerin J. 2005 "Altered exoskeleton composition and vitellogenesis in the crustacean Gammarus sp. collected at polluted sites in the Saguenay Fjord, Quebec, Canada." Environmental Research Vol 98 89 99
11. Godley J. , Brodie R. 2007 "Effect of summer storms on early life stages of Uca minax, U. pugnax and U. pugilator in North Inlet Estuary, South Carolina, USA." Marine Ecology Progress Series Vol 342 197 204
12. Hackney C. T. , Burbanck W. D. , Hackney O. P. 1976 "Biological and physical dynamics of a Georgia tidal creek." Chesapeake Science Vol 17 271 280
13. Hopkins P. M. 2001 "Limb regeneration in the fiddler crab, Uca pugilator: hormonal and growth factor control." American Zoologist Vol 41 389 398[0389:LRITFC]2.0.CO;2
14. Hopkins P. M. , Chung A. C. K. , Durica D. S. 1999 "Regeneration in the fiddler crab, Uca pugilator: histological, physiological, and molecular considerations." American Zoologist Vol 39 513 526
15. Horst M. N. , Walker A. N. 1999 "Effects of the pesticide methoprene on morphogenesis and shell formation in the blue crab Callinectes sapidus." Journal of Crustacean Biology Vol 19 699 707
16. Ismail S. Z. M. , Mykles D. 1992 "Differential molt-induced atrophy in the dimorphic claws of male fiddler crabs, Uca pugnax." Journal of Experimental Zoology Vol 263 18 31
17. Kneib R. T. 1984 "Patterns of invertebrate distribution and abundance in the intertidal salt marsh: causes and questions." Estuaries Vol 7 392 412
18. Kucharski L. C. R. , Da Silva R. S. M. 1991 "Effect of diet composition on the carbohydrate and lipid metabolism in an estuarine crab, Chasmagnathus granulate (Dana, 1851)." Comparative Biochemistry and Physiology Vol 99A 599 602
19. Le Conte J. 1855 "On a new species of Gelasimus." Proceedings of the Academy of Natural Sciences of Philadelphia Vol 7 402 403
20. Lerburg S. B. , Holland A. F. , Sanger D. M. 2000 "Responses of tidal creek macrobenthic communities to the effects of watershed development." Estuaries Vol 23 838 853
21. Mangum C. P. , McMahon B. R. , deFur P. L. , Wheatly M. G. 1985 "Gas exchange, acid-base balance, and the oxygen supply to the tissues during a molt of the blue crab Callinectes sapidus." Journal of Crustacean Biology Vol 5 188 206
22. Mariappan P. , Balasundarum C. , Schmitz B. 2000 "Decapod crustacean chelipeds: an overview." Journal of Bioscience Vol 25 301 313
23. Morris S. 1999 "Integration of physiological responses of crustaceans to environmental challenge." South African Journal of Zoology Vol 33 87 106
24. Mugnier C. , Justou C. 2004 "Combined effect of external ammonia and molt stage on the blue shrimp Litopenaeus stylirostris physiological response." Journal of Experimental Marine Biology and Ecology Vol 309 35 46
25. Mykles D. L. 1980 "The mechanism of fluid absorption at ecdysis in the American lobster, Homarus americanus." Journal of Experimental Biology Vol 84 89 101
26. O'Connor N. J. 1993 "Settlement and recruitment of the fiddler crabs U. pugnax and U. pugilator in a North Carolina, USA salt marsh." Marine Ecology Progress Series Vol 93 227 234
27. Péqueux A. 1995 "Osmotic regulation in crustaceans." Journal of Crustacean Biology Vol 15 1 60
28. Pillai B. R. , Diwan A. D. 2002 "Effects of acute salinity stress on oxygen consumption and ammonia excretion rates of the marine shrimp Metapenaeus monoceros." Journal of Crustacean Biology Vol 22 45 52
29. Pratoomchat B. , Sawangwong P. , Pakkong P. , Machado J. 2002 "Organic and inorganic compound variations in haemolymph, epidermal tissue and cuticle over the molt cycle in Scylla serrata (Decapoda)." Comparative Biochemistry and Physiology Vol 131 243 255
30. Promwikorn W. , Kirirat P. , Intasaro P. , Withyachumnarnkul B. 2007 "Changes in integument histology and protein expression related to the molting cycle of the black tiger shrimp, Penaeus monodon." Comparative Biochemistry and Physiology Vol 148 20 31
31. Roer R. , Dillaman R. 1984 "The structure and calcification of the crustacean cuticle." American Zoologist Vol 24 893 909
32. Saravanan T. S. , Ravindranath M. H. 1981 "Significance of periodic fluctuations in the haemolymph carbohydrates of Scylla serrata (Forskal) during starvation and repeated injury." The Journal of Experimental Zoology Vol 208 139 147
33. Smith S. I. 1870 "Notes on American crustacea. No. 1. Ocypodoidea." Transactions of the Connecticut Academy of Arts and Science Vol 2 113 176
34. Stueckle T. A. , Likens J. , Foran C. M. 2008 "Limb regeneration and molting processes under chronic methoprene exposure in the mud fiddler crab, Uca pugnax." Comparative Biochemistry and Physiology, Part C: Toxicology and Pharmacology Vol 147 366 377
35. Teal J. M. 1958 "Distribution of fiddler crabs in Georgia salt marshes." Ecology Vol 39 185 193
36. Thurman C. L. 2003 "Osmoregulation in fiddler crabs (Uca) from temperate Atlantic and Gulf of Mexico coasts of North America." Marine Biology Vol 142 77 92
37. Vernberg F. J. 1959 "Studies on the physiological variation between tropical and temperate zone fiddler crabs of the genus Uca. III. The influence of temperature acclimation on oxygen consumption of whole organisms." Biological Bulletin Vol 117 582 593
38. Verslycke T. , Janssen C. R. 2002 "Effects of a changing abiotic environment on the energy metabolism in the estuarine mysid shrimp Neomysis integer (Crustacea: Mysidacea)." Journal of Experimental Marine Biology and Ecology Vol 279 61 72
39. Vigh D. A. , Fingerman M. 1985 "Molt staging in the fiddler crab Uca pugilator." Journal of Crustacean Biology Vol 5 386 396
40. Vinagre A. S. , do Amaral A. P. N. , Ribarcki F. P. , da Silveira E. F. , Périco E. 2007 "Seasonal variation of energy metabolism in ghost crab Ocypode quadrata at Siriú Beach." Comparative Biochemistry and Physiology Vol 146 514 519
41. Weis J. S. 1976 "Effects of environmental factors on regeneration and molting in fiddler crabs." Biological Bulletin Vol 150 152 162
42. Weis J. S. 1985 "Cadmium acclimation and limb regeneration in the fiddler crab Uca pugilator sex differences." Marine Environmental Research Vol 16 199 214
43. Weis J. S. , Kim K. 1988 "Tributyltin is a teratogen in producing deformities in limbs of the fiddler crab, Uca pugilator." Archives of Environmental Contamination and Toxicology Vol 17 583 588
44. Wheatly M. G. , Ayers J. 1995 "Scaling of calcium, inorganic contents and organic contents of body mass during the molting cycle of the fresh-water crayfish Procambarus clarkii (Girard)." Journal of Crustacean Biology Vol 15 409 417
45. Wheeler D. E. , Buck N. A. 1992 "Protein, lipid and carbohydrate use during metamorphosis in the fire ant, Solenopsis xyloni." Physiological Entomology Vol 17 397 403

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