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Male Reproductive System Morphology and Spermatophore Formation in Astacus Leptodactylus (Eschscholtz, 1823) (Decapoda: Astacidae)

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Abstract We studied the morphology of the male reproductive system and spermatophore formation in Astacus leptodactylus. The testis has two anterior lobules and only one posterior lobule, which lie dorsal to the gut on the large hepatopancreas. Collecting tubule cells comprise a simple cuboidal epithelium with synthetic activity. Vasa deferentia are laterally connected with the testis and have three parts: proximal vas deferens (PVD), middle vas deferens (MVD) and distal vas deferens (DVD). While the PVD is off-white in color similar to the testis and has no convolutions, the MVD is intensely white in color and convoluted. On the other hand, the DVD is the widest of all and an intensely white structure. The spermatophore, which is non-pedunculate and tubular, extrudes an uninterrupted column and consists of a sperm mass covered with primary and secondary layers. The primary layer is stained with bromephenol blue, while secondary layer gives positive reaction with Alcian blue and aldehyde fuchsin. The histochemical results indicate that the functions of layers are different.

Affiliations: 1: (ME, YT, SSB) Department of Biology, Faculty of Sciences, Istanbul University, 34134, Vezneciler, Istanbul Turkey (


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1. Adiyodi K. G. , Anilkumar G. 1988 "ArthropodaCrustacea. Pp. 261-318." In, K. G. Adiyodi and R. G. Adiyodi (eds.), Reproductive biology of invertebrates: Accessory Sex Glands, Vol. 3. New Delhi, India: Oxford & IBH Publishing
2. Andrews E. A. 1904a "Crayfish spermatozoa." Anatomischer Anzeiger Vol 25 456 463
3. Andrews E. A. 1904b "Breeding habits of crayfish." American Naturalist Vol 38 447 165 206
4. Andrews E. A. 1931 "Spermatophores of an Oregon crayfish." American Naturalist Vol 65 277 280
5. Anilkumar G. , Sudha K. , Subramoniam T. 1999 "Spermatophore transfer and sperm structure in the Brachyuran carb Metopograpsus messor (Decapoda: Grapsidae)." Journal of Crustacean Biology Vol 19 2 361 370
6. Austin C. M. , Ryan S. G. 2002 "Allozyme evidence for a new species of freshwater crayfish of the genus Cherax Erichson (Decapoda: Parastacidae) from the south-west of Western Australia." Invertebrate Systematics Vol 16 357 367
7. Balk İ , ubuk H. , zkk R. , Uysal R. 2005 "Some Biological Characteristics of Crayfish (Astacus leptodactylus Eschscholtz, 1823) in Lake Eğirdir." Turkish Journal of Zoology Vol 29 295 300
8. Bancroft J. D. , Stevens A. 1982 "Theory and Practice of Histological Techniques, pp. 180-216." Churchill Livingstone, London
9. Beach D. , Talbot P. 1987 "Ultrastructure comparison of sperm from the crayfishes Cherax tenuimanus and Cherax albidus." Journal of Crustacean Biology Vol 7 205 218
10. Boone L. 1931 "Anomuran, macruran Crustacea from Panama and the Canal Zone." Bulletin of the American Museum of Natural History Vol 63 137 189
11. Burkenroad M. D. 1938 "The Templeton Crocker Expedition. XIII. Penaeidae from the region of Lower California and Clarion Island with descriptions of four new species." Zoologica, New York Vol 23 53 91
12. Clark E. 1936 "The freshwater and land crayfishes of Australia." Memoirs of the National Museum Vol 10 5 58
13. Cronin L. E. 1947 "Anatomy and histology of the male reproductive system of the Callinectes sapidus, Rathbun." Journal of Morphology Vol 81 2 209 239
14. Dana J. D. 1852 "Conspectus Crustaceorum etc. Conspectus of the Crustcea of the Exploring Expedition under Capt. C. Wilkes, U. S. N." Proceedings of the Academy of Natural Sciences, Philadelphia Vol 6 6 28
15. de Haan W. 1833-1850 "Crustacea." In, P. F. von Siebold (ed.), Fauna Japonica, sive descriptio animalium, quae in itinere per Japoniam, jussu et auspiciis superiorum, qui summum in India Batava Imperium tenent, suscepto, annis 1823-1830 collegit, notis, observationibus et adumbrationibus illustravit: ix-xvi, vii-xvii, i-xxxi, xii-xvii 243 pp. Lugdunum Batavorum
16. de Man J. G. 1879 "On some speciesof the genus Palaemon Fabr. With descriptions of two new forms." Notes Leyden Museum Vol 1 165 184
17. Dudenhausen E. E. , Talbot P. 1979 "Spermiogenesis in the Crayfish, Pacifastacus leniusculus." Journal of Cell Biology Vol 83 225a
18. Dudenhausen E. E. 1982 "An ultrastructural analysis of mature sperm from the crayfish Pacifastacus leniusculusDana." International Journal of Invertebrate Reproduction and Development Vol 5 149 159
19. Dudenhausen E. E. 1983 "An ultrastructural comparison of soft and hardened spermatophores from the crayfish Pacifastacus leniusculus Dana." Canadian Journal of Zoology Vol 61 182 194
20. Eschscholtz F. F. 1823 "Descriptio novae Astacorum speciei Rossicae." Memoires de la Societe Imperiale des Naturalistes du Moscu Vol 6 109 110 1 plate
21. Fabricius J. C. 1798 "Supplementum Entomologiae Systematicae." Hafniae
22. Fasten N. 1914 "Spermatogenesis of American crayfish, Cambarus virilis and Cambarus immunis (?), with special reference to synapsis and the chromatoid bodies." Journal of Morphology Vol 25 4 587 630
23. Faxon W. 1898 "Observation on the Astacidae in the United States National Museum and in the Museum ofComparative Zoology with descriptions of new species." Proceedings of the United States National Museum Vol 20 1136 643 694
24. Fingerman M. 1992 "Glands and Secretion. pp. 345-394." In, F. W. Harrison and A. G. Humes, (eds.), Microscopic Anatomy of Invertebrates Decapod Crustacea. Vol. 10. Wiley-Liss, Inc., New York
25. Girard C. 1852 "A revision of the North American Astaci, with observations on their habits and geographical distribution." Proceedings of the Academy of Natural Sciences of Philadelphia Vol 6 87 91
26. Greenwood J. G. 1972 "The male reproductive system and spermatophore formation in Pagurus novae-zealandiae (Dana) (Anomura: Paguridea)." Journal of Natural History Vol 6 561 574
27. Gner U. , Balk S. 2002 "Relationship between length-weight and egg productivity of crayfishes (Astacus leptodactylus Eschscholtz, 1823) in Lake Ikl (ivril-Denizli), in Turkish. E.U." Journal of Fisheries & Aquatic Sciences Vol 19 (1-2) 109 113
28. Gner U. 2006 "Some morphometric characteristics of crayfish (Astacus leptodactylus Eschscholtz, 1823) in Lake Terkos. E.U." Journal of Fisheries & Aquatic Sciences Vol 23 (1-2) 163 167
29. Haley S. R. 1984 "Spermatogenesis and spermatophore production in the Hawaiian red lobster Enoplometopus occidentalis (Randall) (Crustacea, Nephropidae)." Journal of Morphology Vol 180 181 193
30. Haley S. R. 1986 "Ultrastructure of spermatogenesis in the Hawaiian red lobster, Enoplometopus occidentalis (Randall)." J. Morphol Vol 190 81 92
31. Harloğlu M. M. , Trkgl İ 2000 "The relationship between egg size and female size in freshwater crayfish, Astacus leptodactylus." Aquaculture International Vol 8 95 98
32. Harloğlu M. M. , Holdich D. M. 2001 "Meat yields in the introduced crayfish, Pacifastacus leniusculus and Astacus leptodactylus, from British water." Aquaculture Research Vol 32 411 417
33. Harloğlu M. M. , Aksu 2002 "The importance of sex, individual size and hide size in the hide use of freshwater crayfish (Astacus leptodactylus Eschscholtz, 1823). E.U." Journal of Fisheries & Aquatic Sciences Vol 19 (3-4) 311 317
34. Harloğlu M. M. , Harloğlu A. G. 2004 "The harvest of the freshwater crayfish, Astacus leptodactylus (Eschscholtz, 1823) in Turkey." Reviews in Fish Biology and Fisheries Vol 14 415 419
35. Hinsch G. , Walker H. M. 1974 "The vas deferens of the spider crab, Libinia emarginata." Journal of Morphology Vol 143 1 20
36. Hobbs H. H. Jr. , Harvey M. C. , Hobbs H. H. III. 2007 "A comparative study of functional morphology of the male reproductive systems in the Astacidea with emphasis on the freshwater crayfishes (Crustacea: Decapoda)." Smithsonian Contributions to Zoology Vol 624 1 69
37. Hobbs H. H. Jr. , Hobbs H. H. III. 1990 "A new crayfish (Decapoda: Cambaridae) from southeastern Texas." Proceedings of the Biological Society of Washington Vol 103 608 613
38. Holdich D. M. , Reeve I. D. 1988 "Functional Morphology and Anatomy. pp. 11-51." In, D. M. Holdich and R. S. Lowry (eds.), Freshwater Crayfish: Biology, Management and Exploitation. Croom Helm, London
39. Johnson T. L. 1960 "Histological observations on the sperm duct of Cambarus longulus longulus Girard." Transactions of the American Microscopical Society Vol 79 2 229 238
40. Johnson P. T. 1980 "Histology of the blue crab Callinectes sapidus: A model for decapoda." New York: Praeger
41. Kaye G. I. , Pappas G. D. , Yasuzumi G. , Yamamoto H. 1961 "The distribution and form of the endoplasmic reticulum during spermatogenesis in the crayfish Cambaroides japonicus." Zeitschrift fr Zellforschung und Mikroskopische Anatomie Vol 53 159 171
42. Koelbel K. 1892 "Ein neuer ostasiatischer Flusskrebs." Anzeiger der Kaiserlichen Akademie der Wissenschaften, mathematishenaturwissernschaften Klasse, Wien Vol 29 176 177
43. Kooda-Cisco M. J. , Talbot P. 1982 "A structural analysis of the freshly extruded spermatophore from the lobster, Homarus americanus." Journal of Morphology Vol 172 193 207
44. Krol R. M. , Hawkins W. E. , Overstreet R. M. 1992 "Reproductive Components. pp. 295-343." In, F. W. Harrison and A. G. Humes (eds.), Microscopic Anatomy of Invertebrates. Decapod Crustacea. Vol. 10. Wiley-Liss Inc., New York
45. Latreille P. A. 1804 "Tableau mthodique des Poissons, pp. 71-105." In, Nouveaux Dictionnaire d'Histoire Naturelle, 1re d., v. 24. Paris 1802-04
46. Linnaeus C. 1758 "Systema Naturae per Regna Tria Naturae secundum Classes, Ordinus, Genera, Species cum Characteribus, Differentiis Synonymis, Locis tenth edition. Vol. 1." Holmiae Salvii
47. Lopez-Campos J. , Bargallo R. , Bozzo M. G. , Dufort M. , Fontarnau R. 1981 "The spermiogenesis of crustaceans. VII. Review of spermatozoon of the crayfish Astacus astacus (Malacostraca) Decapoda, Macrura, Reptantia." Gamete Research Vol 4 65 82
48. Lpez-Greco L. S. , Lo Nostro F. L. 2008 "Structural changes in the spermatophore of the freshwater red claw crayfish Cherax quadricarinatus (Von Martens, 1898) (Decapoda, Parastacidae)." Acta Zoologica Vol 89 149 155
49. Lpez-Greco L. S. , Vazquez F. , Rodrguez E. M. 2007 "Morphology of the male reproductive system and spermatophore formation in the freshwater red claw crayfish Cherax quadricarinatus (Von Martens, 1898) (Decapoda, Parastacidae)." Acta Zoologica (Stockholm) Vol 88 223 229
50. Mann T. 1984 "Spermatophores." Springer-Verlag, New York
51. Martens E, von 1869 "Sudbrasilische suss- und brack-wasser Crustaceen nach den sammlungen des Dr. Reinh. Hensel." Archiv fuer Naturgeschichte Vol 35 1 1 35
52. Mazia D. , Brewer P. A. , Alfert M. 1953 "The cytochemical staining and measurement of protein with mercuric bromphenol blue." Biological Bulletin Vol 104 56 67
53. McCroan J. E. Jr. 1940 "Spermatogenesis of the crayfish, Cambarus virilis with special reference to the Golgi material and mitocondria." Cytologia Vol 11 1 136 155
54. McLaughlin P. A. 1983 "Internal anatomy. pp. 1-52." In, F. J. Venberg and W. B. Venberg (eds.), The Biology of Crustacea. Vol. 5. Academic Press, New York
55. Melville-Smith R. 1987 "The reproductive biology of Geryon maritae (Decapoda, Brachyura) off South West Africa/Namibia." Crustaceana Vol 53 259 275
56. Moses M. J. 1961a "Spermiogenesis in the crayfish (Procambarus clarkii). I. Structural characterization of the mature sperm." Journal of Biophysical and Biochemical Cytology Vol 9 222 228
57. Moses M. J. 1961b "Spermiogenesis in the crayfish (Procambarus clarkii). II. Description of stages." Journal of Biophysical and Biochemical Cytology Vol 10 301 333
58. Nagao J. , Munehara H. 2003 "Annual cycle of testicular maturation in the helmet crab Telmessus cheiragonus." Fisheries Science Vol 69 1200 1208
59. Niiyama H. 1934 "The chromosomes of the crayfish, Cambaroides japonicus (de Hann)." Journal of the Faculty of Science, Hokkaido Imperial University (6) Vol 3 2 41 53
60. Noro C. , Lpez-Greco L. S. , Buckup L. 2008 "Gonad morphology and type of sexuality in Parastacus defossus Faxon 1898, a burrowing, intersexed crayfish from Southern Brazil (Decapoda: Parastacidae)." Acta Zoologica Vol 89 59 67
61. Obradovic J. 1989 "Morphological and histological examination of male gonads in the crayfish, Astacus astacus." Cytobios Vol 50 29 38
62. Pearse A. G E. 1960 "Histochemistry theoretical and applied. Vol. 2." J. and A. Churchill, London
63. Penn G. H. Jr. 1943 "A study of the life history of the Louisiana red crayfish, Cambarus clarkii Girard." Ecology Vol 24 1 18
64. Philippi R. A. 1882 "Zoolojia Chilena. Sobre los Astacus. Memorias cientificas1." Literarias, Anales de la Universidad Chile Vol 61 624 628
65. Radaeva I. F. , Kostina G. A. , Il'ina S. G. , Kostyleva R. N. 2001 "Antimicrobial activity of hyaluronic acid." Zhurnal Mikrobiologii, Epidemiologii, I Immunobiologii Vol 1 74 75
66. Radha T. , Subramoniam T. 1985 "Origin and nature of spermatophoric mass of the spiny lobster Panulirus homarus." Marine Biology Vol 86 13 19
67. Randall J. W. 1840 "Catalogue of the Crustacea Brought by Thomas Nuttall and J. K. Townsend, from the West Coast of north America and the Sandwich islands, with Descriptions of Such Species as Are Apparently new, among Which Are included Several Species of Different Localities, Previously Existing in the Collection of the Academy." Journal of the Academy of Natural Sciences of Philadelphia, Series 1 Vol 8 1 106 147
68. Rathbun M. J. 1898 "Description of three new species of freshwater crabs of the genus Potamon." Proceeding of the Biological Society of Washington Vol 12 27 30
69. Rudolph E. 2002 "New records of intersexuality in the freshwater crayfish Samastacus spinifrons (Decapoda, Parastacidae)." Journal of Crustacean Biology Vol 22 377 389
70. Ryan E. P. 1967 "Structure and function of the reproductive system of the crab Portunus sanguinolentus (Herbst) (Brachyura: Portunidae). The male reproductive system." In: Proceedings of Symposium on Crustacea of the Marine Biological Association, India. 506-521
71. Sasikala S. L. , Subramoniam T. 1987 "On the occurrence of acid mucopolysaccharides in the spermatophores of two marine prawns, Penaeus indicus (Milne-Edwards) and Metapenaeus monoceros (Fabricius) (Crustacea: Macrura)." Journal of Experimental Marine Biology and Ecology Vol 113 145 153
72. Scelzo M. A. , Mantelatto F. L. , Tudge C. C. 2004 "Spermatophore morphology of the endemic hermit crab Loxopagurus loxochelis (Anomura, Diogenidae) from the Southwestern Atlantic-Brazil and Argentina." Invertebrate Reproduction and Development Vol 46 1 1 9
73. Shigekawa K. , Clark W. H. Jr. 1986 "Spermiogenesis in the marine shrimp, Sicyonia ingentis." Development, growth and differentiation Vol 28 95 112
74. Smart G. C. 1962 "The life history of the crayfish Cambarus longulus longulus." American Midland Naturalist Vol 68 1 83 94
75. Studitskij A. N. , Eljakova G. C. 1972 "DNA packing in the sperm nuclei of the common crayfish Astacus leptodactylus." Izyvestiya Akademiya Nauk SSSR, seriya Biologiya Vol 4 610 616
76. Subramoniam T. 1991 "Chemical composition of the spermatophores in decapod crustacean." In, R. T. Bauer and J. W. Martin (eds.), Crustacean Sexual Biology, Columbia University Press, New York
77. Subramoniam T. 1995 "Light and electron microscopic studies on the seminal secretions and the vas deferens of the penaeiodean shrimp, Sicyonia ingestis." Journal of Bioscience Vol 20 691 706
78. Suko T. 1955 "Studies on the development of the crayfish III. The development of testis before fertilization." Science Reports of the Saitama University (B) Vol 2 1 39 44
79. Sutcliffe D. 2002 "Book review, Biology of Freshwater Crayfish." In, D. M. Holdich (ed.), Blackwell Science, Qxford, 2002, xvii 702 pages, ISBN 0-632-05431-X Fisheries Research Vol 59 297 299
80. Taketomi Y. , Nishikawa S. , Koga S. 1996 "Testis and androgenic gland during development of external sexual characteristics of the crayfish Procambarus clarkii." Journal of Crustacean Biology Vol 16 24 34
81. Talbot P. , Beach D. 1989 "Role of the vas deferens in the formation of the spermatophore of the crayfish (Cherax)." Journal of Crustacean Biology Vol 9 9 24
82. Tilesius W. G von 1812 "Atlas zur Reise um die Welt unternommen auf Befehl seiner Kaiserlichen Majistt Alexander des Ersten auf den Schiffen Nadeshda und Neva unter dem Commando des Captains von Krusenstern. Atlas." Krusenstern's Atlas
83. Tirelli T. , Campantio E. , Pessani D. , Tudge C. 2007 "Reproductive biology of Mediterranean hermit crabs: male reproductive apparatus of Clibanarius erythropus (Decapoda anomura)." Journal of Crustacean Biology Vol 27 3 404 410
84. Tudge C. C. 1999 "Ultrastructure of the spermatophore lateral ridge in hermit crabs (Decapoda, Anomura, Paguroidea)." Crustaceana Vol 72 1 77 84
85. Wielgus-Serafinska E. 1973 "Structure of the testes in Indigenous crayfishes." Folio Morphologica (Warsaw) Vol 32 1 115 124
86. Wielgus-Serafinska E. 1976 "Morphological and histological changes in the male gonad of American crayfish Orconectes limonus (Rafinesque) in the annual cycle." Acta Biologica Cracoviensia Vol 19 87 105
87. Word B. H. Jr. , Hobbs H. H. Jr. 1958 "Observations on the testis of the crayfish Cambarus montanus acuminatus." Transaction of the American Microscopical Society Vol 77 4 435 450
88. Yasuzumi G. , Kaye G. I. , Pappas G. K. , Yamamoto H. , Tsubo I. 1961 "Nuclear and cytoplasmic differentiation in developing sperm of the crayfish, Cambarus japonicus." Zeitschrift fr Zellforschung und Mikroskopische Anatomie Vol 53 141 158

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