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Linear and Non-Linear Electrical Properties in Peptidergic Neurons of Crayfish Procambarus Clarkii and Cherax Quadricarinatus

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Abstract This work presents a comparative study of the electrophysiological properties of the peptidergic neurons in crayfish Procambarus clarkii and Cherax quadricarinatus. Recordings were made in order to determine the linear and non-linear properties of electrical activity in these neurons. Charge curves were obtained in order to carry out measurements of the input resistance and the determination of the electrotonic length as part of the linear properties of these cells. Different recordings of the action potentials and the ionic currents are presented as part of their non-linear properties. The electrotonic length in P. clarkii was 0.8253 ± 0.1311 and 1.7616 ± 0.4726 in C. quadricarinatus. The input resistance in P. clarkii was 92.4 ± 23.2 MΩ and 56.46 ± 17.2 MΩ in C. quadricarinatus. Silent neurons were recorded (53% in P. clarkii and 60 % in C. quadricarinatus) as well as tonic neurons (35% and 28% in each species respectively) and neurons responding with action potential bursts (12% for both species). We found potassium currents in both species that differ mostly in magnitude. Finally, we discuss the differences that were found in the electrical properties of the neurosecretory cells in crustaceans.

Affiliations: 1: a (RAP) Centro de Investigación en Matemáticas, ICBI, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca_Tulancingo, Km 4.5, Pachuca, Hidalgo, México ( ; 2: b (JAL) Departamento de Ingenieria Electrica, DCBI, Universidad Autónoma Metropolitana-Iztapalapa, Av. Michoacán y Purísima S/N, Mexico, D.F., México ( ; 3: c (RGF) Departamento de Ingenieria Electrica, DCBI, Universidad Autónoma Metropolitana-Iztapalapa, Av. Michoacán y Purísima S/N, Mexico, D.F., México (


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1. Aguilar M. B. , Soyez M. , Falchetto R. , Arnott D. , Shabanowitz J. , Hunt D. F. , Huberman A. 1995 "Amino acid sequence of the minor isomorph of the crustacean hyperglycemic hormone (CHH-II) of the mexican crayfish Procambarus bouvieri (Ortmann): presence of a D-amino acid." Peptides Vol 16 1375 1383
2. Aguilar-Gaytn R. , Cerbn M. A. , Ceballos M. A. , Lizano M. , Huberman A. 1997 "Secuence of a cDNA encoding the molt-inhibiting hormone from the Mexican crayfish Procambarus bouvieri (Crustacea, Decapoda)." Asia Pacific Journal of Molecular Biology and Biotechnology Vol 5 51 55
3. Alvarado R. , Garca U. , Archiga H. 1993 "Electrotonic coupling between neurosecretory cells in the crayfish eyestalk." Brain Research Vol 613 43 48
4. Alvarado-Alvarez R. , Archiga H. , Garca U. 2000 "Serotonin activates a Ca2-dependent K current in identified peptidergic neurons from the crayfish." Journal of Experimental Biology Vol 203 715 723
5. Alvarez O. , Gonzalez C. , Latorre R. 2002 "Counting channels: A tutorial guide on ion channel fluctuation analysis." Advances in Physiology Education Vol 26 327 341
6. Andrew R. , Dudek F. 1984 "Analysis of intracellulary recorded phasic bursting by mammalian neuroendocrine cells." Journal of Neurophysiology Vol 51 552 566
7. Andrew R. , Orchard I. , Saleudin A. 1978 "Structural reevaluation of the neurosecretory system in the crayfish eyestalk." Cells and Tissue Research Vol 190 235 243
8. Archiga H. , Huberman A. , Martmez-Palomo A. 1977 "Release of a neurodepressing hormone from the crustacean sinus gland." Brain Research Vol 128 93 108
9. vila-Pozos R. , Azpiroz J. , Godnez-Fernndez R. 2004 "Estudio comparativo de los patrones de actividad elctrica en las neuronas peptidrgicas de los acociles Procambarus clarkii y Cherax quadricarinatus." Memorias del XXVII Congreso Nacional de Ingeniera Biomdica 74-77
10. Blundon J. , Wright S. , Brodwick M. , Bittner G. 1993 "Residual free calcium is not responsible for facilitation of neurotransmitter release." Proceedings of the National Academy of Sciences Vol 90 9388 9392
11. Charmantier G. , Charmantier-Daures M. , Van Herp F. 1997 "Hormonal Regulation of Growth and Reproduction in Crustaceans, pp. 109-161." In, M. Fingerman, R. Nagabhushanam, and M-F. Thompson (eds.), Recent Advances in Marine Biotechnology: Endocrinology and Reproduction, Science Publishers, New York
12. Chiang R. , Steel C. 1985 "Coupling of electrical activity from contralateral sinus gland." Brain Research Vol 331 142 144
13. Cooke I. , Stuenkel E. 1985 "Electrophysiology of invertebrate neurosecretory cells, pp. 115-164." In, A. Poisner and J. Trifaro (eds.), The Electrophysiology of the Secretory Cell Elsevier, Amsterdam
14. Duan S. , Cooke I. M. 1999 "Selective Inhibition of Transient K Current by La3 in Crab Peptide-Secretory Neurons." Journal of Neurophysiology Vol 81 1848 1855
15. Durand D. 1984 "The somatic shunt cable model for neurons." Biophysical Journal Vol 46 645 653
16. Evans J. , Kember G. , Major G. 1992 "Techniques for obtaining analytical solutions to the multicylinder somatic shunt cable model for passive neurones." Biophysical Journal Vol 63 350 365
17. Garca U. , Archiga H. 1997 "Regulatory influences on crustacean neurosecretory cells." News in Physiological Sciences Vol 12 16 21
18. Garca U. , Archiga H. 1998 "Regulation of crustacean neurosecretory cell activity." Cell and Mollecular Neurobiology Vol 18 81 99
19. Girard C. 1852 "A revision of the North American Astaci, with observations on their habits and geographical distribution." Proceedings of the Academy of Natural Sciences of Philadelphia Vol 6 87 91
20. Greenaway P. , Lawson B. 1982 "Sodium balance in the Austalian crayfish Cherax destructor, Euastacus keirensis and Euastacus spinifer (Decapoda: Parastacoidea)." Australian Journal of Marine and Freshwater Research Vol 33 507 515
21. Hanstrm B. 1939 "Hormones in Invertebrates." Oxford University Press, London
22. Haberman R. 1998 "Elementary Applied Partial Differential Equations with Fourier Series and Boundary Value Problems." Prentice Hall, Englewood Cliffs. 736 pp
23. Hille B. 1992 "Ionic Channels of Excitable Membranes." Sinauer Associates, Inc, Sunderland, MA. 607 pp
24. Hodgkin A. , Huxley A. 1952 "A quantitative description of membrane current and its application to conduction and excitation in nerve." Journal of Physiology Vol 117 500 544
25. Holmes W. R. , Segev I. , Rall W. 1992 "Interpretation of time constant and electrotonic length estimates in multicylinder or branched neuronal structures." Journal of Neurophysiology Vol 68 1401 1420
26. Holmes W. R. , Rall W. 1992 "Electrotonic length estimates in neurons with dendritic tapering or somatic shunt." Journal of Neurophysiology Vol 68 1421 1437
27. Holmes W. R. , Rall W. 1992 "Estimating the electrotonic structure of neurons with compartmental models." Journal of Neurophysiology Vol 68 1438 1452
28. Hoshi T. 1995 "Regulation of voltage dependence of the KAT1 channel by intracellular factors." Journal of General Physiology Vol 105 309 328
29. Huberman A. , Aguilar M. B. , Brew K. , Shabanowitz J. , Hunt D. F. 1993 "Primary structure of the major isomorph of the crustacean hyperglycemic hormone (CHH-I) from the sinus gland of the Mexican crayfish Procambarus bouvieri (Ortmann): Interspecies comparison." Peptides Vol 14 7 16
30. Iwasaki S. , Satow Y. 1971 "Sodium and calcium-dependent spike potentials in the secretory neurons soma of the X-organ of the crayfish." Journal of General Physiology Vol 57 216 238
31. Jars P. 1978 "Tracing of neurosecretory neurons in crayfish optic ganglia by cobalt iontophoresis." Cell and Tissue Research Vol 194 297 302
32. Jiang C. , Haddad G. 1997 "Modulation of K channels by intracellular ATP in human neocortical neurons." Journal of Neurophysiology Vol 77 93 102
33. Johnston D. , Wu S. M-S. 1997 "Foundations of Cellular Neurophysiology." MIT Press, London, UK. 676 pp
34. Kandel E. 1997 "Handbook of Physiology. Vol. 1. The Nervous System." American Physiological Society, Bethesda. 1238 pp
35. Korn S. J. , Horn R. 1989 "Influence of sodium-calcium exchange on calcium current rundown and the duration of calcium-dependent chloride currents in pituitary cells, studied with whole cell and perforated patch recording." Journal of General Physiology Vol 94 789 812
36. Major G. , Evans J. , Jack J. 1993 "Solutions for transients in arbitrarily branching cables: I. voltage recording with somatic shunt." Biophysical Journal Vol 65 423 449
37. McNicholas C. M. , Wang W. , Ho K. , Herbert S. C. , Giebisch G. 1994 "Regulation of ROMK1 K channel activity involves phosphorylation processes." Proceedings of the National Academy of Sciences Vol 91 8077 8081
38. Meade M. , Watts S. 2001 "Physiological compensation in unilateral eyestalk ablated crayfish Cherax quadricarinatus." Journal of Experimental Zoology Vol 289 184 189
39. Nagano M. 1986 "Heterogeneity of neurons in the crustacean X-organ as revealed by intracellular recording and injection of horseradish peroxidase." Brain Research Vol 362 379 383
40. Nagano M. , Cooke I. 1987 "Comparison of electrical response of terminal, axons, and somata of a peptidergic neurosecretory system." Journal of Neuroscience Vol 7 634 648
41. Nsel D. 1996 "Peptidergic neurohormonal control systems in invertebrates." Current Opinion in Neurobiology Vol 6 842 850
42. Onetti C. G. , Garca U. , Valdiosera R. , Archiga H. 1991 "Ionic currents in crustacean neurosecretory cells." Journal of Neurophysiology Vol 64 1514 1526
43. Ortmann A. E. 1909 "Une ecreuisse nouvelle du mexique, Cambarus (Cambarus) bouvieri nov. sp." Annales des Sciences Naturelles, new series Vol 7 159 166
44. Poznanski R. , Bell J. 2000 "A dendritic cable model for the amplification of synaptic potentials by an ensemble average of persistent sodium channels." Mathematical Biosciences Vol 166 101 121
45. Rafinesque C. S. 1817 "Synopsis of four new genera and ten new species of Crustacea found in the United Staes." American Monthly Magazine and Critical Review Vol 2 40 43
46. Rall W. 1969 "Time constants and electrotonic length of membrane cylinders and neurons." Biophysical Journal Vol 9 1483 1508
47. Rall W. 1977 "Core conductor theory and cable properties of neurons, pp. 39-97." In, J. M. Brookhart, V. B. Mountcastle, and S. Geiger (eds.), Handbook of Physiology. The Nervous System. Cellular Biology of Neurons, APS, Bethseda
48. Rall W. 1993 "Transients in neuron with arbitrary dendritic branching and shunted soma." Biophysical Journal Vol 65 15 16
49. Rodrguez-Canto A. , Arredondo-Figueroa J. , Ponce-Palafox J. , Rouse D. 2002 "Growth characteristics of the Australian redclaw crayfish Cherax quadricarinatus, cultured in an indoor recirculating system." Journal of Applied Aquaculture Vol 12 59 64
50. Safronov B. V. , Wolff M. , Vogel W. 2000 "Excitability of the soma in central nervous system neurons." Biophysical Journal Vol 78 2998 3010
51. Sandeman R. , Clarke D. , Sandeman D. , Manly M. 1998 "Growth-related and antennular amputation-induced changes in the olfactory centers of crayfish brain." Journal of Neuroscience Vol 18 6195 6206
52. Sansom S. C. , Stockand J. D. , Hall D. , Williams B. 1997 "Regulation of large calciumactivated potassium channels by protein phosphatase 2A." Journal of Biological Chemistry Vol 272 9902 9906
53. Scott A. 2002 "Neuroscience." A mathematical primer. Springer-Verlag, New York, USA. 360 pp
54. Tang X. D. , Hoshi T. 1999 "Rundown of the hyperpolarization-activated KAT1 channel involves slowing of the opening transitions regulated by phosphorylation." Biophysical Journal Vol 76 3089 3098
55. van Harreveld A. 1936 "A physiological solution for freshwater crustaceans." Proceedings of the Society for Experimental Biology and Medicine Vol 34 428 432
56. Velasco D. , vila-Pozos R. , Godnez R. 2004 "Desarrollo de un paquete de cmputo para la adquisicin de señales bioelctricas a nivel celular, basado en la tarjeta PCI-MIO-16E-1 de National Instruments." Memorias del XXVII Congreso Nacional de Ingeniera Biomdica 98-101
57. Vetter P. , Roth A. , Husser M. 2001 "Propagation of action potential in dendrites depends on dendritic morphology." Journal of Neurophysiology Vol 85 926 937
58. von Martens E. 1868 "ber einige neue Crustaceen." Mber. K. Preuss. Akad. Wiss Vol 1868 608 615

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