Cookies Policy
X

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Genetic Diversity in a Deep-Sea Harpacticoid Copepod Found Near Two Oil-Drilling Sites in the Gulf of Mexico

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Journal of Crustacean Biology

Abstract Sites adjacent to (near-field) and distant from (far-field) oil-drilling platforms were sampled for harpacticoid copepods at two locations in the northern Gulf of Mexico off the coast of Louisiana, both at depths of ≈ 1100 m. The sites were located at Garden Banks Area Block 602 (GB 602) and Mississippi Canyon Area Block 292 (MC 292). Near-field sites at both locations were characterized by large numbers of a single undescribed species of harpacticoid copepod, Bathycletopsyllus sp., but this species was essentially absent from far-field sites. We sequenced a 710 bp portion of the mitochondrial cytochrome c oxidase subunit I gene (COX I) to analyze the genetic diversity and population structure of Bathycletopsyllus sp. at the two platforms, and to determine if the harpacticoids examined were either a single species, or a complex of cryptic species. We found that divergence at the COX I gene (maximum 1.6%) was within the range observed for intraspecific variability in previously-studied species of harpacticoid copepods that were well-characterized taxonomically. Thus, the two samples analyzed here were a single species and not a complex of cryptic species. In addition, there was significant genetic heterogeneity between the two samples, suggesting limited gene flow between the two sites. This was expected given the low dispersal potential typical of harpacticoids and the distance between the sites (407 km). Lastly, haplotype diversity and nucleotide diversity were both low in the GB 602 sample, giving a nominally-significant departure from a pure neutral model. This result could indicate the occurrence of selective sweeps, temporal population size variation or other processes not included in the neutral model. In contrast, haplotype diversity and nucleotide diversity were both higher in the MC 292 sample than at Garden Banks, and there was no detectable departure from neutrality. For both samples, diversity at the haplotype and nucleotide levels were within the range seen in shallow-water harpacticoid species inhabiting both uncontaminated and contaminated muddy sediments, so there was no evidence for pollution related effects in the present study.

Affiliations: 1: (CSG, DWF, correspondence, dfoltz@lsu.edu; JWF) Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803, U.S.A.

Loading

Full text loading...

/content/journals/10.1651/09-3227.1
Loading

Data & Media loading...

1. Baker C. F. 1912 "Notes on the Crustacea of Laguna Beach." Reports of the Laguna Marine Laboratory Vol 1 100 117
2. Belfiore N. M. , Anderson S. L. 2001 "Effects of contaminants on genetic patterns in aquatic organisms: a review." Mutation Research Vol 489 97 122 http://dx.doi.org/10.1016/S1383-5742(01)00065-5
3. Bickford D. , Lohman D. J. , Sodhi N. S. , Ng P. K. L. , Meier R. , Winker K. , Ingram K. K. , Das I. 2007 "Cryptic species as a window on diversity and conservation." Trends in Ecology and Evolution Vol 22 148 155 http://dx.doi.org/10.1016/j.tree.2006.11.004
4. Bickham J. W. , Sandhu S. , Hebert P. D. N. , Chikhi L. , Athwal R. 2000 "Effects of chemical contaminants on genetic diversity in natural populations: implications for biomonitoring and ecotoxicology." Mutation Research Vol 463 33 51 http://dx.doi.org/10.1016/S1383-5742(00)00004-1
5. Brady G. S. 1880 A monograph of the free and semi-parasitic Copepoda of the British Islands Ray Society London
6. Clement M. , Posada D. , Crandall K. A. 2000 "TCS: a computer program to estimate gene genealogies." Molecular Ecology Vol 9 1657 1659 http://dx.doi.org/10.1046/j.1365-294x.2000.01020.x
7. Continental Shelf Associates, Inc 2006 "Effects of Oil and Gas Exploration and Development at Selected Continental Slope Sites in the Gulf of Mexico." Volume II: Technical Report. U.S. Department of the Interior, Minerals Management Service, Gulf of Mexico OCS Region, New Orleans, Louisiana. OCS Study MMS 2006-045. pp.
8. Costa F. O. , deWaard J. R. , Boutillier J. , Ratnasingham S. , Dooh R. T. , Hajibabaei M. , Hebert P. D. N. 2007 "Biological identifications through DNA barcodes: the case of the Crustacea." Canadian Journal of Fisheries and Aquatic Sciences Vol 64 272 295 http://dx.doi.org/10.1139/f07-008
9. Creasy S. S. , Rogers A. D. 1999 "Population genetics of bathyal and abyssal organisms." Advances in Marine Biology Vol 35 1 151
10. Denis F. , Ravallec R. , Pavillon J-F. , van Wormhoudt A. 2009 "Genetic differentiation of Atlantic populations of the intertidal copepod Tigriopus brevicornis." Scientia Marina Vol 73 579 587 http://dx.doi.org/10.3989/scimar.2009.73n3579
11. Etter R. J. , Rex M. A. , Chase M. C. , Quattro J. M. 1999 "A genetic dimension to deep-sea biodiversity." Deep-Sea Research I Vol 46 1095 1099 http://dx.doi.org/10.1016/S0967-0637(98)00100-9
12. Excoffier L. , Smouse P. E. , Quattro J. M. 1992 "Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data." Genetics Vol 131 479 491
13. Fleeger J. W. 1980 "Morphological variation in Cletocamptus(Copepoda: Harpacticoida), with description of a new species from Louisiana salt marshes." Transactions of the American Microscopical Society Vol 99 25 31 http://dx.doi.org/10.2307/3226077
14. Ganz H. H. , Burton R. S. 1995 "Genetic differentiation and reproductive incompatibility among Baja California populations of the copepod Tigriopus californicus." Marine Biology Vol 123 821 827 http://dx.doi.org/10.1007/BF00349126
15. Gómez S. , Fleeger J. W. , Rocha-Olivares A. , Foltz D. W. 2004 "Four new species of CletocamptusSchmankewitsch, 1875, closely related to Cletocamptus deitersi(Richard, 1897) (Copepoda: Harpacticoida)." Journal of Natural History Vol 38 2669 2732 http://dx.doi.org/10.1080/0022293031000156240
16. Grassle J. P. , Grassle J. F. 1976 "Sibling species in the marine pollution indicator Capitella(Polychaeta)." Science Vol 192 567 569 http://dx.doi.org/10.1126/science.1257794
17. Gregg C. , Foltz D. W. , Fleeger J. W. 2006 "Effects of offshore oil and gas development on the genetic diversity of harpacticoid copepods," pp. 441 457 In, Effects of Oil and Gas Exploration and Development at Selected Continental Slope Sites in the Gulf of Mexico Volume II: Technical Report. U.S. Department of the Interior, Minerals Management Service, Gulf of Mexico OCS Region, New Orleans, Louisiana. OCS Study MMS 2006-045. pp.
18. Hebert P. D. N. , Luiker M. M. 1996 "Genetic effects of contaminant exposure – towards an assessment of impacts on animal populations." Science of the Total Environment Vol 191 23 58 http://dx.doi.org/10.1016/0048-9697(96)05169-8
19. Hey J. , Nielsen R. 2007 "Integration within the Felsenstein equation for improved Markov chain Monte Carlo methods in population genetics." Proceedings of the National Academy of Sciences USA Vol 104 2785 2790 http://dx.doi.org/10.1073/pnas.0611164104
20. Hicks G. R. F. , Coull B. C. 1983 "The ecology of marine meiobenthic harpacticoid copepods." Oceanography and Marine Biology, An Annual Review Vol 21 67 175
21. Hummel H. , Patarnello T. 1994 "Genetic effects of pollutants on marine and estuarine invertebrates," 425 434 In, Beaumont A. R. (ed.) Genetics and Evolution of Aquatic Organisms Chapman and Hall London
22. Huys R. , Lee W. 1998/99 "On the relationship of the Normanellidae and the recognition of Cletopsyllidae grad. Nov. (Copepoda, Harpacticoida)." Zoolischer Anzeiger Vol 237 267 290
23. Kim S. J. , Rodiguez-Lanetty M. , Suh J. H. , Song J. I. 2003 "Emergent effects of heavy metal pollution at a population level: Littorina breviculaa study case." Marine Pollution Bulletin Vol 46 74 80 http://dx.doi.org/10.1016/S0025-326X(02)00319-3
24. Knowlton N. 1993 "Sibling species in the sea." Annual Review of Ecology and Systematics Vol 24 189 216 http://dx.doi.org/10.1146/annurev.es.24.110193.001201
25. Knowlton N. 2000 "Molecular genetic analysis of species boundaries in the sea." Hydrobiologia Vol 420 73 90 http://dx.doi.org/10.1023/A:1003933603879
26. Kumar S. , Tamura K. , Jakobsen I. B. , Nei M. 2001 "MEGA2: Molecular Evolutionary Genetics Analysis software." Bioinformatics Vol 17 1244 1245 http://dx.doi.org/10.1093/bioinformatics/17.12.1244
27. Librado P. , Rozas J. 2009 "DnaSP v5: A software for comprehensive analysis of DNA polymorphism data." Bioinformatics Vol 25 1451 1452 http://dx.doi.org/10.1093/bioinformatics/btp187
28. Lobel P. B. , Belkhode S. P. , Jackson S. E. , Longerich H. P. 1990 "Recent taxonomic discoveries concerning the mussel Mytilus: implications for biomonitoring." Archives of Environmental Contamination and Toxicology Vol 19 508 512 http://dx.doi.org/10.1007/BF01059068
29. McDonald T. J. , Brooks J. M. 2006 "Sediment and tissue hydrocarbons," pp. 327 344 In, Effects of Oil and Gas Exploration and Development at Selected Continental Slope Sites in the Gulf of Mexico Volume II: Technical Report. U.S. Department of the Interior, Minerals Management Service, Gulf of Mexico OCS Region, New Orleans, Louisiana. OCS Study MMS 2006-045. pp.
30. Morgan A. J. , Kille P. , Stürzenbaum S. R. 2007 "Microevolution and ecotoxicology of metals in invertebrates." Environmental Science and Technology Vol 41 1085 1096 http://dx.doi.org/10.1021/es061992x
31. Nei M. 1987 Molecular evolutionary genetics Columbia University Press New York
32. Peterson C. H. , Kennicutt M. C. , Green R. H. , Montagna P. , Harper D. E. , Powell E. N. , Roscigno P. F. 1996 "Ecological consequences of environmental perturbations associated with offshore hydrocarbon production: a perspective on long-term exposures in the Gulf of Mexico." Canadian Journal of Fisheries and Aquatic Sciences Vol 53 2637 2654 http://dx.doi.org/10.1139/f96-220
33. Phillips N. W. 2006 "Drilling Activties," pp. 31 56 In, Effects of Oil and Gas Exploration and Development at Selected Continental Slope Sites in the Gulf of Mexico Volume II: Technical Report. U.S. Department of the Interior, Minerals Management Service, Gulf of Mexico OCS Region, New Orleans, Louisiana. OCS Study MMS 2006-045. pp.
34. Phillips N. W. , Hart A. D. 2006 "Study Design and General Methods," pp. 5 29 In, Effects of Oil and Gas Exploration and Development at Selected Continental Slope Sites in the Gulf of Mexico Volume II: Technical Report. U.S. Department of the Interior, Minerals Management Service, Gulf of Mexico OCS Region, New Orleans, Louisiana. OCS Study MMS 2006-045. pp.
35. Poppe S. A. 1881 "Ueber einen neuen Harpacticoiden." Abhandlungen des Naturwissenschaftlichen Vereins zu Bremen Vol 7 149 152
36. Ramos-Onsins S. E. , Rozas J. 2002 "Statistical properties of new neutrality tests against population growth." Molecular Biology and Evolution Vol 19 2092 2100 http://dx.doi.org/10.1093/oxfordjournals.molbev.a004034
37. Richard J. 1897 "Entomostracés de l'Amérique du Sud, recueillis par MM. U. Deiters, H. von Ihering, G. W. Müller et C. O. Poppe." Mémoires de la Société Zoologique de France Vol 10 263 301
38. Rocha-Olivares A. , Fleeger J. W. , Foltz D. W. 2001 "Decoupling of molecular and morphological evolution in deep lineages of a meiobenthic harpacticoid copepod." Molecular Biology and Evolution Vol 18 1088 1102 http://dx.doi.org/10.1093/oxfordjournals.molbev.a003880
39. Rocha-Olivares A. , Fleeger J. W. , Foltz D. W. 2004 "Differential tolerance among cryptic species: a potential cause of pollutant-related reductions in genetic diversity." Environmental Toxicology and Chemistry Vol 23 2132 2137 http://dx.doi.org/10.1897/03-512
40. Ross K. , Cooper N. , Bidwell J. R. , Elder J. 2002 "Genetic diversity and metal tolerance of two marine species: a comparison between populations from contaminated and reference sites." Marine Pollution Bulletin Vol 44 671 679 http://dx.doi.org/10.1016/S0025-326X(01)00333-2
41. Rutledge P. A. , Fleeger J. W. 1988 "Laboratory studies on core sampling with application to subtidal meiobenthos collection." Limnology and Oceanography Vol 33 274 280 http://dx.doi.org/10.4319/lo.1988.33.2.0274
42. Samadi S. , Bottan L. , Macpherson E. , deForges B. R. , Boisselier M. C. 2006 "Seamount endemism questioned by the geographic distribution and population genetic structure of marine invertebrates." Marine Biology Vol 149 1463 1475 http://dx.doi.org/10.1007/s00227-006-0306-4
43. Schizas N. V. , Coull B. C. , Chandler G. T. , Quattro J. M. 2002 "Sympatry of distinct mitochondrial lineages in a copepod inhabiting estuarine creeks in the southeastern USA." Marine Biology Vol 140 585 594 http://dx.doi.org/10.1007/s00227-001-0728-y
44. Schizas N. V. , Chandler G. T. , Coull B. C. , Klosterhaus S. L. , Quattro J. M. 2001 "Differential survival of three mitochondrial lineages of a marine benthic copepod exposed to a pesticide mixture." Environmental Science and Technology Vol 35 535 539 http://dx.doi.org/10.1021/es001219g
45. Schizas N. V. , Street G. T. , Coull B. C. , Chandler G. T. , Quattro J. M. 1997 "An efficient DNA extraction method for small metazoans." Molecular Marine Biology and Biotechnology Vol 6 381 383
46. Schizas N. V. , Street G. T. , Coull B. C. , Chandler G. T. , Quattro J. M. 1999 "Molecular population structure of the marine benthic copepod Microarthridion littoralealong the southeastern and Gulf coasts of the USA." Marine Biology Vol 135 399 405 http://dx.doi.org/10.1007/s002270050640
47. Schneider S. , Roessli D. , Excoffier L. 2000 Arlequin ver 2.000: a software for population genetics data analysis Genetics and Biometry Laboratory, University of Geneva Switzerland
48. Spielman D. , Brook B. W. , Frankham R. , Schaal B. A. 2004 "Most species are not driven to extinction before genetic factors impact them." Proceedings of the National Academy of Sciences USA Vol 101 15261 15264 http://dx.doi.org/10.1073/pnas.0403809101
49. Staton J. L. , Schizas N. V. , Chandler G. T. , Coull B. C. , Quattro J. M. 2001 "Ecotoxicology and population genetics: the emergence of “phylogeographic and evolutionary exotoxicology”." Ecotoxicology Vol 10 217 222 http://dx.doi.org/10.1023/A:1016617410786
50. Staton J. L. , Wickliffe L. C. , Garlitska L. , Villanueva S. M. , Coull B. C. 2005 "Genetic isolation discovered among previously described sympatric morphs of a meiobenthic copepod." Journal of Crustacean Biology Vol 25 551 557 http://dx.doi.org/10.1651/C-2600.1
51. Street G. T. , Montagna P. A. 1996 "Loss of genetic diversity in Harpacticoida near offshore platforms." Marine Biology Vol 126 271 282 http://dx.doi.org/10.1007/BF00347452
52. Theodorakis C. W. 2001 "Integration of genotoxic and population genetic endpoints in biomonitoring and risk assessment." Ecotoxicology Vol 10 245 256 http://dx.doi.org/10.1023/A:1016677629442
53. Van Straalen N. M. , Timmermans M. J. T. N. 2002 "Genetic variation in toxicant-stressed populations: An evaluation of the “genetic erosion” hypothesis." Human and Ecological Risk Assessment Vol 8 983 1002 http://dx.doi.org/10.1080/1080-700291905783
54. Willett C. , Ladner J. 2009 "Investigations of fine-scale phylogeography in Tigriopus californicusreveal historical patterns of population divergence." BMC Evolutionary Biology Vol 9 139 http://dx.doi.org/10.1186/1471-2148-9-139
http://brill.metastore.ingenta.com/content/journals/10.1651/09-3227.1
Loading

Article metrics loading...

/content/journals/10.1651/09-3227.1
2010-11-01
2016-12-09

Sign-in

Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
     
    Journal of Crustacean Biology — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation