Photography of Soft-Bodied Crustaceans via Drying, Whitening, and Splicing
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Abstract It is difficult to take good detailed pictures of soft-bodied crustaceans (and other invertebrates); photographing animals in alcohol leads to problems with lighting and focus while drying out soft-bodied animals for photography distorts their morphology. To help better capture surface detail, the following procedures are proposed. Specimens should be fixed and stained with a general tissue stain in order to ensure an even coloration, followed by either chemical drying (using hexamethyldisilazane) or critical point drying. Specimens are then mounted to allow for whitening with either ammonium chloride (NH4Cl) or magnesium oxide (MgO) to highlight areas of high topographic relief. Lastly, to increase the depth of field in the resulting image, photographs should be taken at sequential planes of focus and then spliced together using a software package for that purpose (i.e., Helicon Focus or Leica Applications Suite). This method is especially useful for showing surface detail and for making comparative images of modern crustaceans to compare with fossil specimens.
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Bray D. 2000 "Critical point drying of biological specimens for scanning electron microscopy" 235 243 Williams J. R. , Clifford A. A. Supercritical Fluid Methods and Protocols Humana Press, Inc Totowa, NJ
Feldmann R. M. 1989 "Whitening fossils for photographic purposes" 342 346 Feldmann R. M. , Chapman R. E. , Hannibal J. T. Paleotechniques
Felgenhauer B. E. 1987 "Techniques for preparing crustaceans for scanning electron microscopy" Journal of Crustacean Biology Vol 7 71 76 http://dx.doi.org/10.2307/1548626
Galigher A. E. , Kozloff E. N. 1971 Essentials of Practical Microtechnique Lea and Febiger Philadelphia
Gibb T. J. , Oseto C. Y. 2006 Arthropod Collection and Identification: Field and Laboratory Techniques Elsevier Academic Press Amsterdam
Green O. R. 2001 A Manual of Practical Laboratory and Field Techniques in Palaeobiology Kluwer Academic Publishers Dordrecht
Heraty J. , Hawks D. 1998 "Hexamethydisilazane—a chemical alternative for drying insects" Entomological News Vol 109 369 374
Laforsch C. , Tollrian R. 2000 "A new preparation technique of daphnids for scanning electron microscopy using hexamethyldisilazane" Archiv für Hydrobiologie Vol 149 587 596
Meyer R. , Melzer R. R. 2004 "Scanning EM diagnosis of marine Decapoda larvae: a comparison of preparation techniques" Crustaceana Vol 77 883 886
Moraes N. , Bouzon Z. L. 1995 "Análise comparative da morfologia externa dos ovos de Macrobrachium potiunacom o uso do hexamethildisilazane e do ponto crítico no microscópio eletrônico de varredura [Comparative analysis of the external morphology of Macrobrachium potiunaeggs by using hexamethildisilazane and critical point in the scanning electron microscopy]" Arquivos de Biologia e Tecnologia Vol 38 689 692
Nation J. L. 1983 "A new method using hexamethyldisilazane for preparation of soft insect tissues for scanning electron microscopy" Stain Technology Vol 58 347 351
Rumph J. A. , Turner W. J. 1998 "Alternative to critical point drying for soft-bodied insect larvae" Annals of the Entomological Society of America (USA) Vol 91 693 699 http://dx.doi.org/10.1093/aesa/91.5.693