Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Amino Acid Analysis in the Shore Crab Carcinus maenas (Decapoda: Brachyura)

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites

image of Journal of Crustacean Biology

Abstract Although amino acid flux models have been developed in fish and other crustaceans, this is the first study to present an amino acid flux model for crabs. This study investigates the influence of different feeds and the deprivation of food on the free amino acid (FAA) pool, the rate of protein turnover and their relation to the growth and flux of amino acids in the shore crab Carcinus maenas. A model was developed for amino acid flux describing food consumption rates, assimilation rates, protein synthesis rates, growth and protein degradation, and losses. Crabs were fed frozen mussel (Mytilus edulis) tissue (Diet 1) or (fresh) white muscle from salmon (Salmo salar) (Diet 2) at 7% of their body weight per day while other crabs were starved for ten days. In the starved crabs, the amino acids partitioned into protein synthesis made up 36% of the free amino acid pool. There was a 50% reduction in the rate of protein synthesis in the starved crabs compared with the fed crabs. It was estimated that daily dietary amino acid intake might compose up to seven times the crabs' FAA pool. In addition, daily protein synthesis and degradation might respectively remove and return the equivalent of up to 4 times and twice the size of the FAA pool in the fed crabs, respectively. Specimens of C. maenas deposited in body proteins (as net growth) 14% in diet 1 and 40% in diet 2 of their amino acid consumption. The amino acid flux in the fed crabs suggested low protein conversion efficiency compared to other decapod crustaceans, including shrimps and lobsters.

Affiliations: 1: (ID) Aberdeen Proteomics, University of Aberdeen, School Medical Sciences, IMS Building Foresterhill, AB25 2ZD Aberdeen, UK; 2: (EM, correspondence,; ITK) University of Thessaly, School of Agricultural Sciences, Department of Ichthyology and Aquatic Environment, Fytoko Street, GR-38446 N. Ionia Magnisias, Greece; 3: (EF, IN) Hellenic Centre for Marine Research, Institute of Aquaculture, Agios Kosmas, Elliniko, GR-16777 Athens, Greece


Full text loading...


Data & Media loading...

1. Ahvenharju T. , Ruohonen K. 2005 "Individual food intake measurement of freshwater crayfish ( Pacifastacus leniusculusDana) juveniles." Aquaculture Research Vol 36 1304 1312
2. Andrés M. , Estévez A. , Anger K. , Rotllant G. 2007 "Growth, survival and biochemical composition of spider crab Maja brachydactylaBalss, 1922 (Decapoda: Majidae) larvae reared under different stocking densities, prey∶larva ratios and diets." Aquaculture Vol 273 494 502
3. Andrés M. , Estévez A. , Anger K. , Rotllant G. 2008 "Developmental patterns of larval growth in the edible spider crab, Maja brachydactyla(Decapoda: Majidae)." Journal Experimental Marine Biology and Ecology Vol 357 35 40
4. Anger K. 2001 The Biology of Decapod Crustacean Larvae. Crustacean Issues: 14 A.A. Balkema Rotterdam
5. Balss H. 1922 "Ostasiatische Decapoden. III. Die Dromiaceen, Oxystomaten und Parthenopiden." Archiv für Naturgeschichte Vol 88A (3) 104 140
6. Bate C. S. 1888 Report on the Crustacea Macrura collected by H. M. S. Challenger during the Years 1873-76. Report on the Scientific Results of the Voyage of H. M. S. Challenger During the Years 1873-76 Under the Command of Captain George S. Nares, R. N., F. R. S. and the Late Captain Frank Tourle Thomson, R. N. Vol. Zoology - XXIV Neill and Company Edinburgh
7. Bishop J. S. , Burton R. S. 1993 "Amino acid synthesis during hyperosmotic stress in Penaeus aztecuspost-larvae." Comparative Biochemistry and Physiology A Vol 106 49 56
8. Boone L. 1931 "A collection of anomuran and macruran Crustacea from the Bay of Panama and the fresh waters of the Canal Zone." Bulletin of the American Museum of Natural History Vol 63 137 189
9. Breteler W. C. M. K. 1975 "Food consumption, growth and energy metabolism of juvenile shore crabs, Carcinus maenas." Netherlands Journal of Sea Research Vol 9 255 272
10. Brousseau D. J. , Baglivo J. A. 2005 "Laboratory investigations of food selection by the Asian shore crab Hemigrapsus sanguineus: algal versus animal preference." Journal of Crustacean Biology Vol 25 130 134
11. Camien M. N. , Sarlet H. , Duchateau G. , Florkin M. 1951 "Non-protein amino acids in muscle and blood of marine and fresh water Crustacea." Journal of Biological Chemistry Vol 193 881 885
12. Carter C. G. , He Z-Y. , Houlihan D. F. , McCarthy I. D. , Davidson I. 1995 "Effect of feeding on the tissue free amino acid concentrations in rainbow trout ( Oncorhynchus mykiss)." Fish Physiology and Biochemistry Vol 14 153 164
13. Castine S. , Southgate P. C. , Zeng C. 2008 "Evaluation of four dietary protein sources for use in microbound diets fed to megalopae of the blue swimmer crab, Portunus pelagicus." Aquaculture Vol 281 95 99
14. Claybrook D. L. 1983 "Nitrogen metabolism," pp. 163 213 In, Mantel L. H. (ed.) The Biology of Crustacea: Internal Anatomy and Physiological Regulation, Vol. 5 Academic Press Inc New York
15. Conceição L. E. C. , van der Meeren T. , Verreth J. A. J. , Evjen M. S. , Houlihan D. F. , Fyhn H. J. 1997 "Amino acid metabolism and protein turnover in larval turbot ( Scophthalamus maximus) fed natural zooplankton or Artemia." Marine Biology Vol 129 255 265
16. Crothers J. H. 1967a "The biology of the shore crab Carcinus maenas(L.). 1. The background-anatomy, growth and life history." Field Studies Vol 2 407 434
17. Crothers J. H. 1967b "The biology of the shore crab Carcinus maenas(L.). 2. The life of the adult crab." Field Studies Vol 2 579 614
18. D'Aniello A. 1980 "Free amino acids in some tissues of marine Crustacea." Experientia Vol 36 392 393
19. Davidson I. 2003 "Hydrolysis of samples for amino acid analysis." In, Smith B. J. (ed.) Methods in Molecular Biology, Protein Sequencing Protocols. Vol 211. 2nd ed Humana Press Totowa, New Jerysey
20. De Graaf J. D. , Tyrrell M. C. 2004 "Comparison of the feeding rates of two introduced crab species, Carcinus maenasand Hemigrapsus sanguineuson the blue mussel Mytilus edulis." Northeastern Naturalist Vol 11 163 167[0163:COTFRO]2.0.CO;2
21. De Haan H. M. 1835 "Crustacea." In, Siebold P. Fvon (ed.) Fauna Japonica sive Descriptio Animalium, Quae in Itinere per Japoniam, Jussu et Auspiciis Superiorum, qui Summum in India Batava Imperium Tenent, Suscepto, Annis 1823-1830 Collegit, Noitis, Observationibus et Adumbrationibus Illustravit Lugduni-Batavorum Leiden
22. De Man J. G. 1879 "On some species of the genus PalaemonFabr. with descriptions of two new forms." Notes from the Leyden Museum Vol 41 165 184
23. Duchateau G. , Florkin M. 1961 "Change in intracellular concentration of free amino acids as a factor of euryhalinity in the crayfish Astacus astacus." Comparative Biochemistry and Physiology Vol 3 245
24. Elner R. W. 1981 "Diet of green crab Carcinus maenas(L.) from Port Hebert, southwestern Nova Scotia." Journal of Shellfish Research Vol 1 84 94
25. Fabricius J. C. 1775 Systema Entomologiae, sistens Insectorum Classes, Ordines, Genera, Species, adjectis Sysnonymis, Locis, Descriptionibus, Observationibus Officina Libraria Kortii Flensburgi et Lipsiae pp.
26. FAO 1992 "Fishery statistics. Catches and landings." Yearbook Fishery Statistics, vol 70. .
27. Fletcher D. J. 1984 "The physiological control of appetite in fish." Comparative Biochemistry and Physiology A Vol 78 617 628
28. Folch J. , Lees M. , Sloane Stanley G. H. 1957 "A simple method for the isolation and purification of total lipids from animal tissues." Journal of Biological Chemistry Vol 266 497 509
29. Forskål P. 1775 "Discriptiones Animalium Avium, Amphibiorum, Piscium, Insectorum, Vermium; quae in Itinere orientali observavit. Petrus Forskål. Post mortem auctoris editit Carsten Niebuhr." Adjuncta est material Medica Kahirina xxxiv. 164 Hafniae.
30. Fraser K. P. P. , Rogers A. D. 2007 "Protein metabolism in marine animals: the underlying mechanisms of growth." Advances in Marine Biology Vol 52 267 362
31. Garlick P. J. , Fem M. , Preedy V. R. 1983 "The effect of insulin infusion and food intake on muscle protein synthesis in postabsorptive rats." Biochemical Journal Vol 210 669 676
32. Garlick P. J. , McNurlan A. M. , Preedy V. R. 1980 "A rapid and convenient technique for measuring the rate of protein synthesis in tissues by injection of [ 3H] phenylalanine." Journal of Biochemistry Vol 192 719 723
33. Gilles R. , Pequeux A. 1983 "Interaction of chemical and osmotic regulation with the environment," pp. 109 177 In, Vernberg F. J. , Vernberg W. B. (eds.) The Biology of Crustacean. Environmental Adaptation, Vol. 8 Academic Press New York
34. Guerao G. , Rotllant G. 2008 "Survival and growth of post-settlement juveniles of the spider crab Maja brachydactyla(Brachyura: Majoidea) reared under individual culture system Aquaculture." Vol 289 181 184
35. Hartnoll R. G. 2001 "Growth in Crustacea-twenty years on." Hydrobiologia Vol 449 11 122
36. Herbst J. F. W. 1782-1804 "Versuch einer Naturgeschichte der Krabben und Krebse, nebst einer systematischen Beschereibung ihrer verschiedenen Arten. Vol. 3(4)." Berlin and Stralsund 1 274, 1-226, 1-216
37. Hird F. J. R. 1986 "The importance of arginine in evolution." Comparative Biochemistry and Physiology B Vol 85 285 288
38. Holme M. H. , Southgate P. C. , Zeng C. 2007 "Survival, development and growth response of mud crab, Scylla serrata(Forskål, 1755), megalopae fed semi-purified diets containing various fish oil∶corn oil ratios." Aquaculture Vol 269 427 435
39. Houlihan D. F. 1991 "Protein turnover in ectotherms and its relationship to energetics," pp. 1 43 In, Gilles R. (ed.) Advances in Comparative and Environmental Physiology, Vol. 7 Springer-Verlag Berlin
40. Houlihan D. F. , Hall S. J. , Gray C. 1989 "Effects of ration on protein turnover in cod." Aquaculture Vol 79 103 110
41. Houlihan D. F. , Carter C. G. , McCarthy I. D. 1995a "Protein turnover in animals," pp. 1 32 In, Walsh P. J. , Wright P. (eds.) Nitrogen Metabolism and Excretion CRC Press Boca Raton
42. Houlihan D. F. , Mathers E. , Foster A. R. 1993 "Biochemical correlates of growth rate in fish," pp. 45 70 In, J. C. , Jensen F. B. (eds.) Fish Ecophysiology Chapman & Hall London
43. Houlihan D. F. , Carter C. G. , McCarthy I. D. , Noble B. S. 1988 "Growth rates and protein turnover in Atlantic cod Gadus morhua." Can. J. Fish. Aquat. Sci. Vol 45 951 964
44. Houlihan D. F. , McCarthy I. D. , Carter C. G. , Marthy F. 1995b "Protein turnover and amino acid flux in fish larvae." ICES Mar. Sci. Symp. Vol 201 87 99
45. Houlihan D. F. , Pedersen B. H. , SteVensen J. F. , Brechin J. 1995c "Protein synthesis, growth and energetics in larval herring ( Clupea harengus) at different feeding regimes." Fish Physiology and Biochemistry Vol 14 195 208
46. Houlihan D. F. , Waring C. P. , Mathers E. , Gray C. 1990 "Protein synthesis and oxygen consumption of the shore crab Carcinus maenasafter a meal." Physiological Zoology Vol 63 735 756
47. Intanai I. , Taylor E. W. , Whiteley N. M. 2009 "Effect of salinity on rates of protein synthesis and oxygen uptake in the post-larvae and juveniles of the tropical prawn Macrobrachium rosenbergii(de Man)." Comparative Biochemistry and Physiology A Vol 152 372 378
48. Jobling M. , Baardvik B. M. , Jorgensen E. H. 1989 "Investigations of food-growth relationships of Arctic charr, Salvelinus alpinusL, using radiography." Aquaculture Vol 81 367 372
49. Kücükgülmez A. , Çelik M. 2008 "Amino acid composition of blue crab ( Callinectes sapidus) from the North Eastern Mediterranean Sea." Journal of Applied Biological Sciences Vol 2 39 42
50. Linnaeus C. 1758 Systema Naturae per Regna Tria Naturae, Secundum Classes, Ordines, Genera, Species, cum Characteribus, Differentiis, Synonymis, Locis (edit. 10). Vol. 1 Laurentii Salvii Holmiae [Stockholm] pp.
51. Lowry O. H. , Rosenbrough N. J. , Farr A. L. , Randall R. J. 1951 "Protein measurement with the folin phenol reagent." Journal of Biological Chemistry Vol 193 265 275
52. Marsden I. D. , Newll R. C. , Ahsanullah M. 1973 "The effect of starvation on the metabolism of the shore crab Carcinus maenas." Comparative Biochemistry and Physiology A Vol 45 195 213
53. McCarthy I. D. , Carter C. G. , Houlihan D. F. 1992 "The effect of feeding hierarchy on individual variability in daily feeding of rainbow trout, Oncorhynchus mykiss(Walbaum)." Journal of Fish Biology Vol 41 257 263
54. McCarthy I. D. , Houlihan D. F. , Carter C. G. , Moutou K. 1993 "Variation in individual food consumption rates of fish and its implications for the study of nutrition and physiology." Proceedings of the Nutrition Society Vol 52 427 436
55. McCoid V. , Miget R. , Finne G. 1984 "Effect of environmental salinity on the free amino acid composition and concentration in penaeid shrimp." Journal of Food Science Vol 49 327 330
56. McGaw I. J. , Reiber C. L. 2000 "Integrated physiological responses to feeding in the blue crab Callinectes sapidus." Journal of Experimental Biology Vol 203 359 368
57. McMillan D. N. , Houlihan D. F. 1988 "The effect of refeeding on tissue protein synthesis in rainbow trout." Physiological Zoology Vol 61 429 441
58. McNamara J. C. , Rosa J. C. , Greene L. J. , Augusto A. 2004 "Free amino acid pools as effectors of osmotic adjustment in different tissues of the freshwater shrimp Macrobrachium olfersii(Crustacea, Decapoda) during long-term salinity acclimation." Marine and Freshwater Behavioral Physiology Vol 37 193 208
59. Mente E. 2003 Nutrition, Physiology and Metabolism in Crustaceans Science Publishers Enfield, New Hampshire
60. Mente E. , Houlihan D. F. , Smith K. 2001 "Growth, feeding frequency, protein turnover, and amino acid metabolism in European lobster Homarus gammarusL." Journal of Experimental Zoology Vol 289 419 432
61. Mente E. , Houlihan D. F. , Coutteau P. , Sorgeloos P. 2002 "Protein turnover, amino acid profile and amino acid flux in juvenile shrimp Litopenaeus vannamei(Boone, 1931): effects of dietary protein source." Journal of Experimental Biology Vol 205 3107 3122
62. Mente E. , Legeay A. , Houlihan D. F. , Massabuau J. C. 2003 "Influence of oxygen partial pressures on protein synthesis in feeding crabs." American Journal of Physiology: Regulatory, Integrative and Comparative Physiology Vol 284 500 510
63. Millward D. J. , Rivers J. 1988 "The nutritional role of indispensable amino acids and the metabolic basis for their requirements." European Journal of Clinical Nutrition Vol 42 367 393
64. Milne Edwards H. 1853 "De la famille des ocypodides (Ocypodidae) suite." Annales des Sciences Naturelles, Série 3 (Zoologie) Vol 20 163 228
65. Naczk M. , Williams J. , Brennan K. , Liyanapathirana C. , Shahidi F. 2004 "Compositional characteristics of green crab Carcinus maenas." Food Chemistry Vol 88 429 434
66. Rathbun M. J. 1986 "The genus Callinectes." Proceedings of the United States National Museum Vol 18 349 375
67. Sampedro M. P. , González-Gurriarán E. , Freire J. 2003 "Moult cycle and growth of Maja squinado(Decapoda: Majidae) in coastal habitats of Galicia, north-west Spain." Journal of the Marine Biological Association U.K. Vol 83 995 1005
68. Sánchez-Paz A. , Garcıa-Carreno F. , Muhlia-Almazan A. , Peregrino-Uriarte B. A. , Hernandez-Lopez J. , Yepiz-Plascencia G. 2006 "Usage of energy reserves in crustaceans during starvation: Status and future directions." Insect Biochemistry and Molecular Biology Vol 36 241 249
69. Schacterle G. R. , Pollock R. L. 1973 "A simplified method for the quantitative assay of small amounts of protein in biological material." Analytical Biochemistry Vol 51 654 655
70. Schoffeniels E. , Gilles R. 1970 "Nitrogenous constituents and nitrogen metabolism in arthropods," pp. 199 227 In, Florkin M. , Scheer B. T. (eds.) Chemical Zoology, vol. 5 Academic Press New York
71. Skonberg D. I. , Perkins B. L. 2002 "Nutrient composition of green crab ( Carcinus maenas) leg meat and claw meat." Food Chemistry Vol 77 401 404
72. Smith S. I. 1870 "Notes on American Crustacea. No. 1. Ocypodoidea." Transactions of the Connecticut Academy of Arts and Science Vol 2 113 176
73. Tacon A. G. J. 1996 "Nutritional studies in crustaceans and the problems of applying research findings to practical farming systems." Aquaculture Nutrition Vol 1 165 174
74. Teshima S. , Ishikawa M. , Koshio S. 2000 "Nutritional assessment and feed intake of microparticulate diets in crustaceans and fish." Aquaculture Research Vol 31 691 702
75. Thomas C. W. , Carter C. G. , Crear B. J. 2002 "Potential use of radiography for measuring feed intake of southern rock lobster ( Jasus edwardsii)." Journal of Experimental Marine Biology and Ecology Vol 273 189 198
76. Vernberg F. J. 1959 "Studies on the physiological variation between tropical and temperate zone fiddler crabs of the genus Uca. II. Oxygen consumption of whole organisms." Biological Bulletin of the Marine Biological Laboratory Vol 117 163 184
77. Wallace J. C. 1972 "Activity and metabolic rate in the shore crab Carcinus maenas." Comparative Biochemistry and Physiology A Vol 41 523 535
78. Wallace J. C. 1973 "Feeding, starvation and metabolic rate in the shore crab Carcinus maenas." Marine Biology Vol 20 277 281
79. Walton M. J. 1985 "Aspects of amino acid metabolism in teleost fish," pp. 47 67 In, Cowey C. B. , Mackie A. M. , Bell J. G. (eds.) Nutrition and Feeding in Fish Academic Press London
80. Watts D. C. 1968 "Variation in enzyme structure and function: the guidelines of evolution," pp. 1 114 In, Lowenstein O. (ed.) Advances in Comparative Physiology and Biochemistry, Vol. 3 Academic Press New York
81. Ways P. , Hanahan D. J. 1964 "Characterisation and quantification of red cell lipids in normal man." Journal of Lipid Research Vol 5 318 328
82. Wickins J. F. , Lee D. O'C. 2002 Crustacean Farming: Ranching and Culture, 2nd edit Blackwell London
83. Wilson R. P. , Poe W. E. 1985 "Relationship of whole body and egg essential amino acid patterns to amino acid requirement patterns in catfish, Ictalurus punctatus." Comparative Biochemistry and Physiology B. Vol 80 385 388
84. Woll A. K. , van der Meeren G. I. , Tuene S. 2006 "Quality improvement by feeding wild-caught edible crab ( Cancer pagurusL.): a pilot study." Aquaculture Research Vol 37 1487 1496
85. Zar J. H. 1996 Biostatistical Analysis Prentice-Hall London
86. Zmora O. , Findiesen A. , Stubblefield J. , Frenkel V. , Zohar Y. 2005 "Large-scale juvenile production of the blue crab Callinectes sapidus." Aquaculture Vol 244 129 139

Article metrics loading...



Can't access your account?
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation