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Novel Polymorphic Microsatellite Markers in Scylla paramamosain and Cross-Species Amplification in Related Crab Species

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Abstract Scylla paramamosain is a commercially important crab species in China. Here we reported 12 novel polymorphic microsatellite markers isolated in S. paramamosain using PCR-based isolation of microsatellite arrays (PIMA). The analysis of genetic variability was performed in a sample of 29 individuals. A total of 82 alleles were detected with an average of 6.8 alleles per locus. The number of alleles, polymorphism information content (PIC), observed and expected heterozygosity per locus ranged from three to 12, from 0.42 to 0.88, from 0.38 to 1.00 and from 0.48 to 0.91, respectively. Three loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction (P < 0.0042) and no significant linkage disequilibrum between pairs of loci was found. Cross-species amplification of these markers was evaluated in three closely related crab species S. tranquebarica, S. olivacea, and S. serrata. This study will potentially be useful for stock management, constructing of a genetic linkage map, mapping economically important quantitative trait loci (QTL), and evaluating the population genetic diversity of S. paramamosain.

Affiliations: 1: Key Lab of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, 200090, China

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1. Botstein D. , White R. L. , Skolnick M. , Davis R. W. 1980 "Construction of a genetic linkage map in man using restriction fragment length polymorphisms" American Journal of Human Genetics Vol 32 314 331
2. Brookfield J. F. Y. 1996 "A simple new method for estimating null allele frequency from heterozygote deficiency" Molecular Ecology Vol 5 453 455 http://dx.doi.org/10.1046/j.1365-294X.1996.00098.x
3. Chiang T. Y. , Ju Y. M. , Fang L. S. , Lin C. J. 2009 "Isolation and characterization of polymorphic microsatellite loci in Candidia barbata(Cyprinidae) using PCR-based isolation of microsatellite arrays (PIMA)" Conservation Genetics Vol 10 503 505 http://dx.doi.org/10.1007/s10592-008-9513-5
4. Cifarelli R. A. , Gallitelli M. , Cellini F. 1995 "Random amplified hybridization microsatellites (rahm)-isolation of a new class of microsatellite-containing DNA clones" Nucleic Acids Research Vol 23 3802 3803 http://dx.doi.org/10.1093/nar/23.18.3802
5. Cowan L. 1985 Crab farming in Japan, Taiwan and the Philippines Queensland Department of Primary Industries Queensland, Australia
6. Ender A. , Schwenk K. , Stadler T. , Streit B. , Schierwater B. 1996 "RAPD identification of microsatellites in Daphnia " Molecular Ecology Vol 5 437 441 http://dx.doi.org/10.1111/j.1365-294X.1996.tb00333.x
7. Estampador E. P. 1949 "Studies on Scylla(Crustacea: Portunidae). I. Revision of the genus" Philippine Journal of Science Vol 78 95 108
8. Fabricius J. C. 1798 Supplementum Entomologiae Systematicae Hafniae
9. Forskål P. 1775 "Discriptiones Animalium Avium, Amphibiorum, Piscium, Insectorum, Vermium; quae in Itinere orientali observavit" Petrus Forskål. Post mortem auctoris editit Carsten Niebuhr. Adjuncta est material Medica Kahirina: xxxiv, pp. Hafniae
10. Guyomar R. , Mauger S. , Tabet-Canale K. , Martieau S. , Genet C. , Krieg F. , Quillet E. 2006 "A type I and II microsatellite linkage map of rainbow trout ( Oncorhynchus mykiss) with presumptive coverage of all chromosome arms" BMC Genomics Vol 7 302 314 http://dx.doi.org/10.1186/1471-2164-7-302
11. Herbst J. F. W. 1796 (1782-1804) "Versuch einer Naturgeschichte der Krabben und Krebse, nebst einer systematischen Beschereibung ihrer verschiedenen Arten" Vol 2 1 226 Berlin and Stralsund
12. Hsu K. C. , Wang J. P. , Chen X. L. , Chiang T. Y. 2004 "Isolation and characterization of microsatellite loci in Acrossocheilus paradoxus(Cyprinidae) using PCR-based isolation of microsatellite arrays (PIMA)" Conservation Genetics Vol 5 113 115 http://dx.doi.org/10.1023/B:COGE.0000014051.67562.55
13. Keenan C. P. 1999 Aquaculture of the mud crab, genus Scylla past, present and future. In: Mud Crab Aquaculture and Biology. ACIAR Proceedings Vol 78 9 13 Watson Ferguson and Company Canberra, Australia
14. Li Q. , Wan J. M. 2005 "SSRHUNTER: Development of a local searching software for SSR sites" Hereditas Vol 27 808 810
15. Lin H. D. , Lee T. W. , Lin F. J. , Lin C. J. , Chiang T. Y. 2008 "Isolation and characterization of microsatellite loci in the endangered freshwater fish Pararasbora moltrechti(Cyprinidae) using PCR-based isolation of microsatellite arrays (PIMA)" Conservation Genetics Vol 9 945 947 http://dx.doi.org/10.1007/s10592-007-9365-4
16. Liu Y. G. , Bao B. L. , Liu L. X. , Wang L. , Lin H. 2008 "Isolation and characterization of polymorphic microsatellite loci from RAPD product in half-smooth tongue sole ( Cynoglossus semilaevis) and a test of cross-species amplification" Molecular Ecology Notes Vol 8 202 204 http://dx.doi.org/10.1111/j.1471-8286.2007.01923.x
17. Lunt D. H. , Hutchinson W. F. , Carvalho G. R. 1999 "An efficient method for PCR-based isolation of microsatellite arrays (PIMA)" Molecular Ecology Vol 8 891 893 http://dx.doi.org/10.1046/j.1365-294X.1999.00636.x
18. Ma H. Y. , Yang J. F. , Su P. Z. , Chen S. L. 2009b "Genetic analysis of gynogenetic and common populations of Verasper moseriusing SSR markers" Wuhan University Journal of Natural Sciences Vol 14 267 273 http://dx.doi.org/10.1007/s11859-009-0315-5
19. Ma H. Y. , Bi J. Z. , Shao C. W. , Chen Y. , Miao G. D. , Chen S. L. 2009a "Development of 40 microsatellite markers in spotted halibut ( Verasper variegatus) and the cross-species amplification in barfin flounder ( Verasper moseri)" Animal Genetics Vol 40 576 578 http://dx.doi.org/10.1111/j.1365-2052.2009.01869.x
20. Ma L. B. , Zhang F. Y. , Ma C. Y. , Qiao Z. G. 2006 " Scylla paramamosain(Estampador) the most common mud crab (Genus Scylla) in China: evidence from mtDNA" Aquaculture Research Vol 37 1694 1698 http://dx.doi.org/10.1111/j.1365-2109.2006.01603.x
21. McDonald G. J. , Danzmann R. G. , Ferguson M. M. 2004 "Relatedness determination in the absence of pedigree information in three cultured strains of rainbow trout ( Oncorhynchus mykiss)" Aquaculture Vol 233 65 78 http://dx.doi.org/10.1016/j.aquaculture.2003.08.003
22. Obata Y. , Imai H. , Kitakado T. , Hamasaki K. , Kitada S. 2006 "The contribution of stocked mud crabs Scylla paramamosainto commercial catches in Japan, estimated using a genetic stock identification technique" Fisheries Research Vol 80 113 121 http://dx.doi.org/10.1016/j.fishres.2006.03.016
23. O'Reilly P. , Wright J. M. 1995 "The evolving technology DNA fingerprinting and its application to fisheries and aquaculture" Journal of Fish Biology Vol 47 (suppl A) 29 55 http://dx.doi.org/10.1111/j.1095-8649.1995.tb06042.x
24. Pettay D. T. , Lajeunesse T. C. 2009 "Microsatellite loci for assessing genetic diversity, dispersal and clonality of coral symbionts in ‘stress-tolerant’ clade D Symbiodinium " Molecular Ecology Resources Vol 9 1022 1025 http://dx.doi.org/10.1111/j.1755-0998.2009.02561.x
25. Rice W. R. 1989 "Analyzing tables of statistical tests" Evolution Vol 43 223 225 http://dx.doi.org/10.2307/2409177
26. Sun Y. F. , Sun H. Y. , Zhao Y. , Qian G. H. , Zhao Q. , Liu N. F. 2009 "Isolation and characterization of microsatellite loci in the freshwater crab Sinopotamon yangtsekienseand cross-species amplification in related taxa (Decapoda: Brachyura)" Journal of Crustacean Biology Vol 29 273 274 http://dx.doi.org/10.1651/08-3115.1
27. Takano M. , Barinova A. , Sugaya T. , Obata Y. , Watanabe T. , Ikeda M. , Taniguchi N. 2005 "Isolation and characterization of microsatellite DNA markers from mangrove crab, Scylla paramamosain " Molecular Ecology Notes Vol 5 794 795 http://dx.doi.org/10.1111/j.1471-8286.2005.01065.x
28. Van Oosterhout C. , Hutchinson W. F. , Wills D. P. M. , Shipley P. 2004 "MICRO-CHECKER: software for identifying and correcting genotyping errors in mirosatellite data" Molecular Ecology Notes Vol 4 535 538 http://dx.doi.org/10.1111/j.1471-8286.2004.00684.x
29. Weber J. L. , May P. E. 1989 "Abundant class of human DNA polymorphisms which can be typed using the polymerase chain-reaction" American Journal of Human Genetics Vol 44 388 396
30. Xu X. J. , Wang G. Z. , Wang K. J. , Li S. J. 2009 "Isolation and characterization of ten new polymorphic microsatellite loci in the mud crab, Scylla paramamosain " Conservation Genetics Vol 10 1877 1878 http://dx.doi.org/10.1007/s10592-009-9843-y
31. Yeh F. C. , Yang R. C. , Boyle T. 1999 "POPGENE version 1.31. Microsoft window-bases freeware for population genetic analysis" Available: ( ). University of Alberta and the Centre for International Forestry Research.
32. Zhan A. , Hu J. , Hu X. , Hui M. , Wang M. , Peng W. , Huang X. , Wang S. , Lu W. , Sun C. , Bao Z. 2009 "Construction of microsatellite-based linkage maps and identification of size-related quantitative trait loci for Zhikong scallop ( Chlamys farreri)" Animal Genetics Vol 40 821 831 http://dx.doi.org/10.1111/j.1365-2052.2009.01920.x
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/content/journals/10.1651/09-3263.1
2010-01-01
2016-12-08

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