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Isolation and Characterization of a Ferritin cDNA from the Mud Crab Scylla paramamosain

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Abstract Ferritin is an important protein for iron storage in cells. A hepatopancreas cDNA library from the mud crab Scylla paramamosain was constructed using the SMART technique. A complete cDNA sequence that showed high identity with the conserved sequence of the ferritin gene was cloned from the cDNA library and subjected to further investigation. The full-length ferritin gene of Scylla paramamosain (SpFer) consists of 767 bp and contains a complete open reading frame of 513 bp and a 26-bp iron-respective element in the 5′-untranslated region. The gene encodes a polypeptide of 170 amino acids, constituting a predicted molecular weight of 19.44 kDa and a theoretical isoelectric point of 5.24. The deduced protein shares 84% identity with the ferritin protein of the crab Eriocheir sinensis. Quantitative real-time PCR analyses revealed that the expression of ferritin was ubiquitous in different organs of S. paramamosain, including muscle, heart, ovary, testis, and hepatopancreas. The highest expression level was found in the heart, while testis tissue showed the lowest level. Ferritin mRNA expression in continuous developmental stages in zoeal phases, including Z1, Z2, Z3, Z4, and Z5, as well as megalopa and juvenile crab I stages, were also examined by quantitative real-time PCR. The expression level of ferritin was highest in the Z1 stage and lowest in the megalopa stage. This study provides useful information regarding the structure and function of ferritin and will play an important role in immunity and resistance research in S. paramamosain.

Affiliations: 1: (DZ; KJ; FZ; CM; YS; ZQ; LM, malingbo@vip.sina.com) East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 300 Jungong Road, Shanghai 200090, People's Republic of China

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1. Andersen O. , Dehli A. , Standal H. , Giskegjerde T. A. , Karstensen R. , Rorvik K. A. 1995 "Two ferritin subunits of Atlantic salmon ( Salmon salar): cloning of the liver cDNA and antibody preparation" Molecular Marine Biology and Biotechnology Vol 4 164 170
2. Andrews S. C. , Harrison P. M. , Yewdall S. J. , Arosio P. , Levi S. , Bottke W. , Von Darl M. , Briat J. F. , Laulhe J. P. , Lobreaux S. 1992 "Structure, function, and evolution of ferritins" Journal of Inorganic Biochemistry Vol 47 161 174 http://dx.doi.org/10.1016/0162-0134(92)84062-R
3. Arnold K. , Bordoli L. , Kopp J. , Schwede T. 2006 "The SWISS-MODEL Workspace: A web-based environment for protein structure homology modeling" Bioinformatics Vol 22 195 201 http://dx.doi.org/10.1093/bioinformatics/bti770
4. Arosio P. , Ingrassia R. , Cavadini P. 2009 "Ferritins: A family of molecules for iron storage, antioxidation and more" Biochemiacaet Biophysica Acta (BBA) - General Subjects Vol 1790 589 599 http://dx.doi.org/10.1016/j.bbagen.2008.09.004
5. Beck G. , Ellis T. W. , Habicht G. S. , Schluter S. F. , Marchalonis J. J. 2002 "Evolution of the acute phase response: iron release by echinoderm ( Asterias forbesi) coelomocytes, and cloning of an echinoderm ferritin molecule" Developmental & Comparative Immunology Vol 26 11 26 http://dx.doi.org/10.1016/S0145-305X(01)00051-9
6. Chen X. W. , Shi Z. Y. , Cheng Q. Q. 2009 "Full-length cDNA cloning and tissues expression analysis of Ferritin gene from Acipenser sinensis " Zoological Research Vol 30 144 150 http://dx.doi.org/10.3724/SP.J.1141.2009.02144
7. De Man J. G. 1879 "On some species of the genus PalaemonFabr. with descriptions of two new forms" Notes from the Leyden Museum Vol 41 165 184
8. de Zoysa M. , Lee J. 2007 "Two ferritin subunits from disk abalone ( Haliotis discus discus): Cloning, characterization and expression analysis" Fish & Shellfish Immunology Vol 23 624 635 http://dx.doi.org/10.1016/j.fsi.2007.01.013
9. Durand J. P. , Goudard F. , Pieri J. , Escoubas J. M. , Schreiber N. , Cadoret J. P. 2004 " Crassostrea gigasferritin: cDNA sequence analysis for two heavy chain type subunits and protein purification" Gene Vol 338 187 195 http://dx.doi.org/10.1016/j.gene.2004.04.027
10. Estampador E. P. 1949 "Studies on Scylla(Crustacea, Portunidae). Revision of the genus" Philippines Journal of Sience Vol 78 95 108
11. Graham R. M. , Chua A. C. G. , Herbison C. E. , Olynyk J. K. , Trinder D. 2007 "Liver iron transport" World Journal of Gastroenterology Vol 13 4725 4736
12. Harrison P. M. , Arosio P. 1996 "Ferritin: structure, gene regulation, and cellular function in animals, plants, and micro-organisms" Annual Review of Biochemistry Vol 56 289 315
13. Harrison P. M. , Arosio P. 1996 "The ferritins: molecular properties, iron storage function and cellular regulation" Biochimica et Biophysica Acta (BBA) – Bioenergetics Vol 1275 161 203 http://dx.doi.org/10.1016/0005-2728(96)00022-9
14. Hentze M. , Kuhn L. C. 1996 "Molecular control of vertebrate iron metabolism: mRNA-based regulatory circuits operated by iron, nitric oxide and oxidative stress" Proceedings of the National Academy of Sciences Vol 93 8175 8182 http://dx.doi.org/10.1073/pnas.93.16.8175
15. Imai H. , Takeda M. 2005 "A natural hybrid mud crab (Decapoda: Portunidae) from Japan" Journal of Crustacean Biology Vol 25 620 624 http://dx.doi.org/10.1651/C-2493.1
16. Imjongjirak C. , Amparyup P. , Tassanakajon A. , Sittipraneed S. 2007 "Antilipopolysaccharide factor (ALF) of mud crab Scylla paramamosain: Molecular cloning, genomic organization and the antimicrobial activity of its synthetic LPS binding domain" Molecular Immunology Vol 44 3195 3203 http://dx.doi.org/10.1016/j.molimm.2007.01.028
17. Lawson D. M. , Treffry A. , Artymiuk P. J. , Harrison P. M. , Yewdall S. J. , Luzzago A. , Cesareni G. , Levi S. , Arosio P. 1989 "Identification of the ferroxidase centre in ferritin" FEBS Letters Vol 254 207 210 http://dx.doi.org/10.1016/0014-5793(89)81040-3
18. Li M. , Saren G. , Zhang S. 2008 "Identification and expression of a ferritin homolog in amphioxus Branchiostoma belcheri: Evidence for its dual role in immune response and iron metabolism" Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol 150 263 270 http://dx.doi.org/10.1016/j.cbpb.2008.03.014
19. Liu H. P. , Chen R. Y. , Zhang M. , Wang K. J. 2010 "Isolation, gene cloning and expression profile of a pathogen recognition protein: A serine proteinase homolog (Sp-SPH) involved in the antibacterial response in the crab Scylla paramamosain " Developmental & Comparative Immunology Vol 34 741 748 http://dx.doi.org/10.1016/j.dci.2010.02.005
20. Ma L. B. , Zhang F. Y. , Ma C. Y. , Qiao Z. G. 2006 " Scylla paramamosain(Estampador) the most common mud crab (Genus Scylla) in China: evidence from mtDNA" Aquaculture Research Vol 37 1694 1698 http://dx.doi.org/10.1111/j.1365-2109.2006.01603.x
21. Milne Edwards H. 1853 "De la famille des ocypodides (Ocypodidae) suite" Annales des Sciences Naturelles, Série 3 (Zoologie) Vol 20 163 228
22. Orino K. , Eguchi K. , Nakayama T. , Yamamoto S. , Watanabe K. 1997 "Sequencing of cDNA Clones that encode bovine ferritin H and L chains" Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol 118 667 673 http://dx.doi.org/10.1016/S0305-0491(97)00277-0
23. Orino K. , Lehman L. , Tsuji Y. , Ayaki H. , Torti S. V. , Torti F. M. 2001 "Ferritin and the response to oxidative stress" Biochemistry Vol 357 241 247 http://dx.doi.org/10.1042/0264-6021:3570241
24. Qiu G. F. , Zheng L. , Liu P. 2008 "Transcriptional regulation of ferritin mRNA levels by iron in the freshwater giant prawn, Macrobrachium rosenbergii " Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol 150 320 325 http://dx.doi.org/10.1016/j.cbpb.2008.03.016
25. Recalcati S. , Invernizzi P. , Arosio P. , Cairo G. 2008 "New functions for an iron storage protein: The role of ferritin in immunity and autoimmunity" Journal of Autoimmunity Vol 30 84 89 http://dx.doi.org/10.1016/j.jaut.2007.11.003
26. Rucker P. , Torti F. M. , Torti S. V. 1996 "Role of H and L subunits in mouse ferritin" Journal of Biological Chemistry Vol 271 33352 33357 http://dx.doi.org/10.1074/jbc.271.52.33352
27. Santambrogio P. , Levi S. , Cozzi A. , Corsi B. , Arosio P. 1996 "Evidence that the specificity of iron incorporation into homopolymers of human ferritin L- and H-chains is conferred by the nucleation and ferroxidase centres" Biochemical Journal Vol 314 139 144
28. Schwede T. , Kopp J. , Guex N. , Peitsch and M. C. 2003 "SWISS-MODEL: an automated protein homology-modeling server" Nucleic Acids Research Vol 31 3381 3385 http://dx.doi.org/10.1093/nar/gkg520
29. Tamura K. , Dudley J. , Nei M. , Kumar and S. 2007 "MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0" Molecular Biology and Evolution Vol 24 1596 1599 http://dx.doi.org/10.1093/molbev/msm092
30. Theil E. C. 1987 "Ferritin: structure, gene regulation, and cellular function in animals, plants, and microorganisms" Annual Review of Biochemistry Vol 56 289 315 http://dx.doi.org/10.1146/annurev.bi.56.070187.001445
31. von Darl M. , Harrison P. M. , Bottke W. 1994 "cDNA cloning and deduced amino acid sequence of two ferritins: Soma ferritin and yolk ferritin, from the snail Lymnaea stagnalis " European Journal of Biochemistry Vol 222 353 366 http://dx.doi.org/10.1111/j.1432-1033.1994.tb18874.x
32. Waldo G. S. , Ling J. , Loehr J. S. , Theil E. C. 1993 "Formation of an Fe(III)-tyrosinate complex during biomineralization of H-subunit ferritin" Science Vol 259 796 798 http://dx.doi.org/10.1126/science.8430332
33. Wang X. , Liu B. , Xiang J. 2009 "Cloning, characterization and expression of ferritin subunit from clam Meretrix meretrixin different larval stages" Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol 154 12 16 http://dx.doi.org/10.1016/j.cbpb.2009.04.011
34. Yedery R. D. , Reddy K. V. R. 2009 "Identification, cloning, characterization and recombinant expression of an anti-lipopolysaccharide factor from the hemocytes of Indian mud crab, Scylla serrata " Fish & Shellfish Immunology Vol 27 275 284 http://dx.doi.org/10.1016/j.fsi.2009.05.009
35. Zhang Y. , Meng Q. , Jiang T. , Wang H. , Xie L. , Zhang R. 2003 "A novel ferritin subunit involved in shell formation from the pearl oyster ( Pinctada fucata)" Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol 135 43 54 http://dx.doi.org/10.1016/S1096-4959(03)00050-2
36. Zheng L. 2007 "The expression of ferritin mRNA induced by iron in giant freshwater prawn Macrobrachium rosenbergii " Marine Fisheries Vol 29 307 313 (in Chinese with English abstract)
37. Zheng W. J. , Hu Y. H. , Xiao Z. Z. , Sun L. 2010 "Cloning and analysis of a ferritin subunit from turbot ( Scophthalmus maximus)" Fish & Shellfish Immunology Vol 28 829 836 http://dx.doi.org/10.1016/j.fsi.2010.01.013
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2011-05-01
2016-10-01

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