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Molecular-Based Method To Distinguish the Sibling Species Hemigrapsus penicillatus and Hemigrapsus takanoi (Decapoda: Brachyura: Varunidae)

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Abstract In this study, we developed a simple and rapid restriction fragment length polymorphism (RFLP) method to distinguish the sibling species Hemigrapsus penicillatus and H. takanoi based on interspecific base substitutions within the 16S rRNA region of mitochondrial DNA. Sequencing and alignment of partial sequences revealed two haplotypes for H. penicillatus and one for H. takanoi, and five species-specific base substitutions. Digestion of the PCR products using the restriction endonuclease Dde I produced a banding pattern that was consistent with species identification based on morphological and pigmentation patterns. The sequence for H. takanoi collected in Japan showed high homology with that reported for specimens collected in Europe. This method could be useful for identification of these two species by researchers without specific taxonomic knowledge.

Affiliations: 1: National Research Institute of Far Seas Fisheries, Fisheries Research Agency, 5-7-1 Orido, Shimizu, Shizuoka, Shizuoka Prefecture 424-8633, Japan; 2: Department of Aquaculture, Faculty of Fisheries and Marine Science, Sam Ratulangi University, Jl. Kampus Unsrat, Bahu Manado 95115, Indonesia; 3: Department of Marine Biosciences, Faculty of Marine Science, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan


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1. Asakura A. 2006 "The story behind the discovery of Hemigrapsus takanoiAsakura and Watanabe, 2005, a cryptic species of crab, previously thought to be the common Japanese intertidal crab H. penicillatus(Decapoda: Brachyura: Grapsoidea)" TAXA, Proceedings of the Japanese Society of Systematic Zoology Vol 21 33 39 [in Japanese with English abstract.]
2. Asakura A. , Watanabe S. 2005 " Hemigrapsus takanoi, new species, a sibling species of the common Japanese intertidal crab H. penicillatus(Decapoda: Brachyura: Grapsoidea)" Journal of Crustacean Biology Vol 25 279 292
3. Asakura A. , Mingkid W. , Yamasaki I. , Watanabe S. 2008 "Revalidation of Hemigrapsus takanoiAsakura Watanabe, 2005: A rebuttal to “Sakai (2007): Comments on an invalid nominal species, Hemigrapsus takanoiAsakura Watanabe, 2005, a synonym of Hemigrapsus penicillatus(De Haan, 1835) (Decapoda, Brachyura, Grapsidae).”" Crustaceana Vol 81 1263 1273
4. Breton G. , Faasse M. , Noël P. , Vincent T. 2002 "A new alien crab in Europe: Hemigrapsus sanguineus(Decapoda: Brachyura: Grapsidae)" Journal of Crustacean Biology Vol 22 184 189
5. Dauvin J-C. 2009 "Establishment of the invasive Asian shore crab Hemigrapsus sanguineus(De Haan, 1835) (Crustacea: Brachyura: Grapsoidea) from the Cotentin Peninsular, Normandy, France" Aquatic Invasions Vol 4 467 472
6. Dauvin J-C. , Rius A. T. , Ruellet T. 2009 "Recent expansion of two invasive crabs species Hemigrapsus sanguineus(de Haan, 1835) and H. takanoiAsakura and Watanabe 2005 along the Opal Coast, France" Aquatic Invasions Vol 4 451 465
7. De Haan W. 1833-1850 "Crustacea" pp. i -xxxi, ix-xvi, 1-243, pls. A-J, L-Q, 1-55, tables 1-2 In, Von Siebold I. P. F. (ed.) Fauna Japonica sive Descriptio Animalium, quae in itinere per Japoniam, jussu et auspiciis superiorum, qui summum in India Batavia imperium tenent, suscepto, annis 1823-1830 collegit, notis, observationibus et adumbrationibus illustravit P. F. de Siebold. Conjunctis studiis C. J. Temminck et H. Schlegel pro Vertebratis atque W. de Haan pro Invertebratis elaborata Regis aupicus edita Lugduni-Batavorum Leiden
8. Doi W. , Iinuma Y. , Yokota M. , Watanabe S. 2009 "Comparative feeding behavior of invasive ( Carcinus aestuarii) and native crabs ( Hemigrapsus takanoi)" Crustacean Research Vol 38 1 11
9. Gollasch S. 1999 "The Asian decapod Hemigrapsus penicillatus(de Haan, 1835) (Grapsidae, Decapoda) introduced in European waters: status quo and future perspecitive" Helgoländer Meeresuntersuchungen Vol 52 359 366
10. Lee S. H. , Ko H. S. 2008 "First zoeal stages of six species of Hemigrapsus(Decapoda: Brachyura: Grapsidae) from the northern Pacific including an identification key" Journal of Crustacean Biology Vol 28 675 685
11. Micu D. , Niţă V. , Todorova V. 2010 "First record of the Japanese shore crab Hemigrapsus sanguineus(de Haan, 1835) (Brachyura: Grapsoidea: Varunidae) from the Black Sea" Aquatic Invasions Vol 5 suppl. 1 S1 S4
12. Mingkid W. M. , Akiwa S. , Watanabe S. 2006a "Morphological characteristics, pigmentation, and distribution of the sibling penicillate crabs, Hemigrapsus penicillatus(De Haan, 1835) and H. takanoiAsakura & Watanabe, 2005 (Decapoda, Brachyura, Grapsidae) in Tokyo Bay" Crustaceana Vol 79 1107 1121
13. Mingkid W. M. , Yokota M. , Watanabe S. 2006b "Salinity tolerance of larvae in the penicillate crab Hemigrapsus takanoi(Decapoda: Brachyura; Grapsidae)" La Mer Vol 44 17 21
14. Noel P. Y. , Tardy E. , D'Acoz C. D. 1997 "Will the crab Hemigrapsus penicillatusinvade the coasts of Europe?" Life Sciences Vol 320 741 745
15. Sakai K. 2007 "Comments on an invalid nominal species, Hemigrapsus takanoiAsakura Watanabe, 2005, a synonym of Hemigrapsus penicillatus(De Haan, 1835) (Decapoda, Brachyura, Grapsidae)" Crustaceana Vol 80 503 508
16. Schubart C. D. 2003 "The East Asian shore crab Hemigrapsus sanguineus(Brachyura: Varunidae) in the Mediterranean Sea: an independent human-mediated introduction" Scientia Marina Vol 67 195 200
17. Schubart C. D. , Cuesta J. A. , Rodríguez A. 2001 "Molecular phylogeny of the crab genus Brachynotus (Brachyura: Varunidae) based on the 16S rRNA gene" Hydrobiologia Vol 449 41 46
18. Schubart C. D. , Diesel R. , Hedges S. B. 1998 "Rapid evolution to terrestrial life in Jamaican crabs" Nature Vol 393 363 365
19. Schubart C. D. , Neigel J. E. , Felder D. L. 2000a "Use of the mitochondrial 16S rRNA gene for phylogenetic and population studies of Crustacea" pp. 817 830 In, Von Vaupel Klein J. C. , Schram F. R. (eds.) Crustacean Issue 12 The Biodiversity Crisis and Crustacea. A. A. Balkema Rotterdam
20. Schubart C. D. , Neigel J. E. , Felder D. L. 2000b "Molecular phylogeny of mud crabs (Brachyura: Panopeidae) from the northwestern Atlantic and the role of morphological stasis and convergence" Marine Biology Vol 137 11 18
21. Shinji J. , Strüssmann C. A. , Wilder M. N. , Watanabe S. 2009 "Short-term responses of the adults of the common Japanese intertidal crab, Hemigrapsus takanoi(Decapoda: Brachyura: Grapsoidea) at different salinities: osmoregulation, oxygen consumption, and ammonia excretion" Journal of Crustacean Biology Vol 29 269 272
22. Takano M. , Ikeda M. , Kijima A. 1997 "Biochemical and morphological evidence of two sympatric forms, interpreted as sibling species, in the estuarine grapsid crab Hemigrapsus penicillatus(De Haan)" Benthos Research Vol 52 111 117
23. Tamura K. , Dudley J. , Nei M. , Kumar S. 2007 "MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0" Molecular Biology and Evolution Vol 24 1596 1599
24. Yamasaki I. , Yoshizaki G. , Yokota M. , Strüssmann C. A. , Watanabe S. 2006 "Mitochondrial DNA variation and population structure of the Japanese mitten crab Eriocheir japonica " Fisheries Science Vol 72 299 309

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