Two Methods for Determining the Fertility Status of Early-Stage American Lobster, Homarus americanus, Eggs
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Abstract The American lobster (Homarus americanus Milne Edwards, 1837) is the focus of the most important commercial fishery in New England, which relies on a variety of biological monitoring programs and surveys to guide the development of appropriate management plans. One key piece of information provided by these surveys is the number of females that are carrying eggs (ovigerous) that will subsequently contribute new recruits to the fishery. A major assumption is that all eggs carried by ovigerous females are fertilized and will thus result in viable recruits. However, because some lobsters extrude, and briefly carry, unfertilized eggs, this assumption needs to be re-evaluated. In particular, it is important to determine the approximate proportion of newly extruded eggs that are either fertilized, or not. The major goal of this project was to develop reliable methods for determining if early-stage lobster eggs (live and preserved) were in fact fertilized. One method involved using a nucleic acid stain to visualize egg DNA, after pretreatment of eggs with a proteolytic and collagenolytic enzyme solution to facilitate stain penetration through the egg membrane. With this method multi-nucleated (fertilized) eggs could be clearly distinguished from unfertilized eggs. A total of 20 egg clutches were tested to determine their fertility status using this method. Of these, 16 clutches (80%) were fertilized while 4 were not fertilized (20%). Of the 16 clutches with fertilized eggs, two had a mix of both fertilized and unfertilized eggs. A second method, using fluorometry to obtain measurements of total egg DNA, was also developed. There was a significant difference between the total DNA concentration in unfertilized control oöcytes and early-stage fertilized eggs (P < 0.001), and the total amount of DNA gradually increased as eggs developed (r = 0.961, P < 0.0001). Both of these methods will make it possible to make a more accurate assessment of the proportion of female lobsters that will actually contribute new recruits to the fishery.
Affiliations:
1: University of New Hampshire, Department of Biological Sciences, 46 College Road, Durham, New Hampshire 03824, U.S.A
1. AikenD. E. , WaddyS. L.1980 "Reproductive biology" pp.215276In,CobbJ. S. , PhillipsB. F.(eds.)The Biology and Management of Lobsters. Vol. 1Academic PressNew York
2. AikenD. E. , WaddyS. L.1982 "Cement gland development, ovary maturation, and reproductive cycles in the American lobster Homarus americanus " Journal of Crustacean Biology Vol 2 315327 http://dx.doi.org/10.2307/1548050
3. Atlantic States Marine Fisheries Commission (ASMFC)Stock Assessment Report No. 09-01 (supplement). March 2009.
5. ButtinoI. , IanoraA. , CarotenutoY. , ZupoV. , MiraltoA.2003 "Use of confocal laser scanning microscope in studies on the developmental biology of marine crustaceans" Microscopy Research and Technique Vol 6 458464 http://dx.doi.org/10.1002/jemt.10284
6. CheungT. S.1966 "The development of the egg membranes and egg attachment in the shore crab, Carcinus maenas, and some related decapods" Journal of the Marine Biological Association UK Vol 46 373400 http://dx.doi.org/10.1017/S0025315400027181
7. ChittleboroughR. G.1976 "Breeding of Panulirus longipes cygnusGeorge under natural and controlled conditions" Australian Journal of Marine and Freshwater Research Vol 27 499516 http://dx.doi.org/10.1071/MF9760499
9. FAO Fisheries Dept., Data and Statistical unit2009FISHSTAT Plus: Universal software for fishery statistical time series.
10. FogartyM. J.1995 "Populations, fisheries, and management" pp.111133In,FactorJ. R.(ed.)Biology of the Lobster Homarus americanusAcademic PressNew York
11. GeorgeR. W.1962 "Description of Palinurus cygnussp. nov., the commercial crayfish (or spiny lobster) of Western Australia" Journal of the Royal Society of Western Australia Vol 45 100110
12. GosselinT. , Sainte-MarieB. , BernatchezL.2003 "Patterns of sexual cohabitation and female ejaculate storage in the American lobster ( Homarus americanus)" Behavioral Ecology and Sociobiology Vol 55 151160 http://dx.doi.org/10.1007/s00265-003-0695-z
13. GosselinT. , Sainte-MarieB. , BernatchezL.2005 "Geographic variation of multiple paternity in the American lobster, Homarus americanus " Molecular Ecology Vol 14 15171525 http://dx.doi.org/10.1111/j.1365-294X.2005.02498.x
14. HankinD. G. , ButlerT. H. , WildP. W. , XueQ. L.1997 "Does intense fishing on males impair mating success of female Dungeness crabs?" Canadian Journal of Fisheries and Aquatic Sciences Vol 54 655669 http://dx.doi.org/10.1139/f96-308
15. HedgecockD.1983 "Maturation and spawning of the American lobster, Homarus americanus. " Crustacean Aquaculture Vol 1 261270
16. HelluyS. , BeltzB. S.1991 "Embryonic development of the American lobster Homarus americanus:Quantitative staging and characterization of an embryonic molt cycle" Biological Bulletin Vol 180 355371 http://dx.doi.org/10.2307/1542337
17. HerrickF. H.1895 "The American lobster: A study of its habits and development" Bulletin U. S. Fisheries Commission Vol 15 1252
18. HerrickF. H.1909 "Natural history of the American lobster" Bulletin U.S. Bureau of Fisheries Vol 29 149408
19. HinesA. H. , JivoffP. R. , BushmannP. J. , van MontfransJ. , ReedS. A. , WolcottD. L. , WolcottT. G.2003 "Evidence for sperm limitation in the blue crab, Callinectes sapidus " Bulletin of Marine Science Vol 72 287310
20. KendallM. S. , WolcottD. L. , WolcottT. G. , HinesA. H.2002 "Influence of male size and mating history on sperm content of ejaculates of the blue crab Callinectes sapidus " Marine Ecology Progress Series Vol 230 235240 http://dx.doi.org/10.3354/meps230235
21. KnightA. P.1918 "Lobster investigations at Long Beach Pond, N.S" Contributions to Canadian Biology5371
22. LandersD. F.Jr , KeserM. , SailaS. B.2001 "Changes in female lobster ( Homarus americanus) size at maturity and implications for the lobster resource in Long Island Sound, Connecticut" Journal of Marine and Freshwater Research Vol 52 12831290 http://dx.doi.org/10.1071/MF01045
23. LittleS. A. , WatsonW. H.III2005 "Differences in the size at maturity of female American lobsters, Homarus americanus, captured throughout the range of the offshore fishery" Journal of Crustacean Biology Vol 25 585592 http://dx.doi.org/10.1651/C-2552.1
24. MacDiarmidA. , ButlerM. J.IV1999 "Sperm economy and limitation in spiny lobsters" Behavioral Ecology and Sociobiology Vol 46 1424 http://dx.doi.org/10.1007/s002650050587
25. MacDiarmidA. , StewartR.2005 "Male but not female mate choice by the red rock lobster is influenced by numbers of potential mates in a den" The Lobster Newsletter Vol 18 89
26. MasciJ. , MonteiroA.2005 "Visualization of early embryos of the butterfly Bicyclus anynana " Zygote139144
27. Milne EdwardsH.1834-1837 "Histoire naturelle des Crustacés comprenant l'anatomie, la physiologie et la classification de ces animaux" Paris, Librairie Encyclopédique de Roret1638
28. Milne EdwardsH. , LucasH.1841 "Description des Crustacés nouveaux ou peu connus conserves dans la collection du museum" Archives du Muséum national d'Histoire naturelle Paris Vol 2 461483
29. PerkinsH. C.1971 "Egg loss during incubation from offshore northern lobsters (Decapoda: Homaridae)" Fishery Bulletin Vol 69 451453
30. PerkinsH. C.1972 "Developmental rates at various temperatures of embryos of the northern lobster ( Homarus americanusMilne-Edwards)" Fishery Bulletin Vol 70 9599
31. PollockD. E.1993 "Recruitment overfishing and resilience in spiny lobster populations" ICES Journal of Marine Science Vol 50 914 http://dx.doi.org/10.1006/jmsc.1993.1002
32. RondeauA. , Sainte-MarieB.2001 "Variable mate-guarding time and sperm allocation by male snow crab ( Chionoecetes opilio) in response to sexual competition, and their impact on the mating success of females" Biological Bulletin Vol 201 204217 http://dx.doi.org/10.2307/1543335
33. Sainte-MarieB. , CarriereC.1995 "Fertilization of the second clutch of eggs of snow crab, Chionoecetes opilio,from females mated once or twice after their molt to maturity" Fishery Bulletin Vol 93 759764
34. SasakiG. , CapuzzoJ. M. , BiesiotP.1986 "Nutritional and bioenergetic considerations in the development of the American lobster Homarus americanus " Canadian Journal of Fisheries and Aquatic Sciences Vol 43 23112319 http://dx.doi.org/10.1139/f86-283
35. SatoT. , GoshimaS.2007 "Female choice in response to risk of sperm limitation by the stone crab, Hapalogaster dentata " Animal Behavior Vol 73 331338 http://dx.doi.org/10.1016/j.anbehav.2006.05.016
36. SatoT. , AshidateM. , JinboT. , GoshimaS.2006 "Variation of sperm allocation with male size and recovery rate of sperm numbers in spiny king crab Paralithodes brevipes " Marine Ecology Progress Series Vol 312 189199 http://dx.doi.org/10.3354/meps312189
37. TalbotP.1981 "Collagenase solutions induce in-vitro ovulation in lobsters ( Homarus americanus)" Journal of Experimental Zoology Vol 216 181185 http://dx.doi.org/10.1002/jez.1402160120
38. TalbotP. , GoudeauM.1988 "A complex cortical reaction leads to formation of the fertilization envelope in the lobster, Homarus americanus. " Gamete Research Vol 19 118 http://dx.doi.org/10.1002/mrd.1120190102
39. TalbotP. , HarperR.1984 "Abnormal egg stalk morphology is correlated with clutch attrition in laboratory-maintained lobsters ( Homarus americanus)" Biological Bulletin Vol 166 349356 http://dx.doi.org/10.2307/1541221
41. TemplemanW.1940 "Embryonic development rates and egg laying of Canadian lobsters" Journal of the Fisheries Research Board of Canada Vol 5 7183 http://dx.doi.org/10.1139/f40-009
42. UglemI. , UksnoyL. E. , BerghO.1996 "Chemical treatment of lobster eggs against epibiotic bacteria" Aquaculture International Vol 4 18 http://dx.doi.org/10.1007/BF00175216
43. WaddyS. L. , AikenD. E.1985 "Fertilization and egg retention in artificially inseminated female American lobsters, Homarus americanus " Canadian Journal of Fisheries and Aquatic Sciences Vol 42 19541956 http://dx.doi.org/10.1139/f85-242
44. WaddyS. L. , AikenD. E.2005 "Impact of invalid biological assumptions and misapplication of maturity criteria on size-at-maturity estimates for American lobster" Transactions of the American Fisheries Society Vol 134 10751090 http://dx.doi.org/10.1577/T03-216.1
45. WaddyS. L. , AikenD. E. , De KlejinD. P. V.1995 "Control of growth and reproduction" pp.217266In,FactorJ. R.(ed.)Biology of the Lobster Homarus americanusAcademic PressNew York
46. ZirbelM. J. , MillerC. B. , BatchelderH. P.2007 "Staging egg development of marine copepods with DAPI and PicoGreen®" Limnology and Oceanography Methods Vol 5 106110 http://dx.doi.org/10.4319/lom.2007.5.106